LR Grade TBE Buffer
Laboratory Buffer Grade
Laboratory Reagent

LR Grade TBE Buffer

High-quality Laboratory Reagent grade TBE Buffer specifically formulated for electrophoresis applications. Our LR grade buffer delivers consistent pH and ionic strength for reliable DNA separation and molecular biology applications requiring stable electrophoretic conditions.

  • pH 8.3 ± 0.1 Stability
  • 10X Concentrate
  • Consistent Ionic Strength
  • Electrophoresis Grade
  • Laboratory Reagent Quality
  • Ready-to-Dilute Format
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Technical Specifications

Product Type: TBE electrophoresis buffer
Full Name: Tris-Borate-EDTA buffer
Tris Concentration: 89 mM (10.8 g/L)
Boric Acid: 89 mM (5.5 g/L)
EDTA: 2 mM (0.74 g/L)
pH Value: 8.3 ± 0.1
Ionic Strength: 180 mM
Conductivity: 18.0 ± 1.0 mS/cm
Concentration: 10X concentrated stock
Appearance: Clear, colorless solution
Working Dilution: 1X (dilute 1:10)
DNase Activity: Not detected
RNase Activity: Not detected
Heavy Metals: ≤ 0.1 ppm
Endotoxin Level: ≤ 0.01 EU/mL
Package Sizes: 100mL, 500mL, 1L bottles
Storage: Room temperature
Shelf Life: 36 months at room temperature

Applications

DNA Gel Electrophoresis
RNA Electrophoresis
PCR Product Analysis
Restriction Fragment Analysis
DNA Fragment Isolation
Molecular Cloning
Educational Laboratories
Quality Control Testing
Clinical Diagnostics
Plant Genetics Research
DNA Quantification
Forensic DNA Analysis

Industry-Specific Grades

DRAVYOM offers specialized TBE buffer formulations tailored for specific electrophoresis applications, ensuring optimal resolution, conductivity, and nuclease-free quality for diverse molecular biology and diagnostic requirements.

Molecular Biology Grade
Concentration: 10X stock DNase/RNase: Free pH: 8.3 ± 0.05 Application: Research
High Resolution
Conductivity: Optimized Clarity: Ultra-clear Resolution: Enhanced Application: Fine separation
Clinical Grade
GMP: Manufactured Endotoxin: ≤ 0.005 EU/mL Validation: Complete Application: Diagnostics
Educational Grade
Volume: Large containers Cost: Economical Quality: Reliable Application: Teaching labs

Quality Standards

DRAVYOM's TBE buffer is manufactured under stringent quality control protocols, meeting international molecular biology standards including ISO 13485, nuclease-free specifications, and industry requirements for electrophoresis applications.

ISO 9001:2015 Certified Manufacturing
ISO 13485 Medical Device Quality
pH 8.3 ± 0.1 Certified
Nuclease Activity Testing
DNase/RNase Free Verified
≤ 0.1 ppm Heavy Metals
Electrophoresis Performance Certificate
Ambient Temperature Shipping

Advanced Chemical Properties & Performance

TBE Buffer exhibits exceptional chemical properties essential for DNA/RNA electrophoresis applications. Its precisely balanced composition and optimal ionic strength ensure reliable performance in demanding gel electrophoresis and nucleic acid analysis procedures.

Buffer Composition
Tris Base: 89 mM (10.8 g/L)
Boric Acid: 89 mM (5.5 g/L)
EDTA (Na₂): 2 mM (0.74 g/L)
pH at 25°C: 8.3 ± 0.1
Physical Properties
Ionic Strength: 0.089 M at 1X concentration
Conductivity: 2.8 mS/cm at 25°C
Density: 1.002 g/cm³ at 20°C
Appearance: Clear, colorless solution
Electrophoresis Properties
Current Density: 5-10 V/cm typical
Resolution Range: 50 bp - 20 kb optimal
Buffer System: Tris-boric acid-EDTA
DNA Migration: Consistent linear separation
Quality Specifications
Sterility: Sterile filtered (0.22 μm)
Endotoxin Level: ≤ 10 EU/mL
Heavy Metals: ≤ 5 ppm
Nuclease Activity: Not detected
Stability Properties
Shelf Life: 2 years at 4°C
Working Solution: Stable 6 months at RT
pH Stability: ±0.1 pH units over time
Precipitation: No visible precipitate

Performance Characteristics

Detailed performance metrics demonstrate TBE Buffer superiority in electrophoresis applications with exceptional pH stability, optimal ionic strength, and consistent separation performance for demanding nucleic acid analysis procedures.

pH Stability

Buffer: pH 8.3 ± 0.1 maintained

Exceptional pH buffering capacity
Ionic Strength

Strength: 0.089 M optimal conductivity

Ideal electrophoretic conditions
Resolution

Range: 50 bp - 20 kb optimal separation

Broad nucleic acid size range
Nuclease-Free

Activity: No nuclease contamination

Protected nucleic acid integrity
Stability

Storage: 2 years at 4°C

Extended laboratory reliability
Sterility

Quality: 0.22 μm sterile filtered

Molecular biology grade

Safety Information

Generally safe for laboratory use but may cause mild eye and skin irritation upon direct contact. Contains EDTA which can chelate metal ions. Use appropriate laboratory protective equipment including safety goggles and gloves. Avoid ingestion and ensure adequate ventilation. Follow standard molecular biology laboratory safety procedures.

Laboratory Chemical
Eye Irritant
Standard Precautions

Storage & Handling

Store at 4°C in original containers away from light and temperature fluctuations. Use sterile techniques when dispensing to prevent contamination. Allow to reach room temperature before use for optimal performance. Check for precipitation before use and filter if necessary.

Store at 4°C
Sterile handling techniques
Protect from light
Equilibrate to room temperature

Chemical Mechanisms & Reaction Pathways

LR TBE Buffer exhibits precise electrophoretic mechanisms through Tris-borate-EDTA buffering system, enabling superior DNA separation and gel electrophoresis performance for molecular biology applications.

Buffer System

Tris-borate pH buffering maintains optimal electrophoretic conditions

Stable pH for consistent separation
Ion Mobility

Controlled ionic strength for optimal DNA migration

Enhanced electrophoretic resolution
EDTA Chelation

Metal ion chelation prevents nuclease activity

DNA integrity preservation
Electrical Conductivity

Optimized conductivity for efficient DNA separation

Improved band resolution

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global molecular biology access with complete documentation supporting international electrophoresis and research standards.

ISO 9001

International quality management system certification

FDA Compliance

Food and Drug Administration research use only

GMP Standards

Good Manufacturing Practice buffer production

CE Marking

European Conformity for research applications

Performance Validation

Supported electrophoresis performance documentation

SDS Documentation

Multi-language Safety Data Sheets (GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's electrophoresis specialists provide comprehensive TBE Buffer support, gel optimization, and technical services for molecular biology applications.

Protocol Development
  • Electrophoresis method optimization
  • Gel concentration protocols
  • Voltage and timing optimization
  • Custom buffer solutions
Quality Testing
  • pH stability testing
  • Conductivity measurement
  • Resolution assessment
  • Contamination screening
Technical Support
  • Electrophoresis troubleshooting
  • Gel optimization
  • Molecular biology protocols
  • Research collaboration
Supply Solutions
  • Research scheduling
  • Emergency supply
  • Multiple concentrations
  • Academic distribution

Environmental Impact & Sustainability

Our LR TBE Buffer production emphasizes environmental responsibility through sustainable buffer chemistry, waste minimization, and comprehensive environmental management.

Buffer Recovery

Controlled disposal and chemical recovery systems

Water Quality

Ultra-pure water systems with minimal environmental impact

Efficient Processing

Optimized buffer preparation minimizes waste

Safe Formulation

Non-toxic buffer systems for laboratory safety

Green Chemistry

Environmentally preferred electrophoresis buffers

Container Recovery

Buffer container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's molecular biology facility employs advanced buffer chemistry to ensure consistent LR TBE Buffer quality for critical electrophoresis applications.

Chemical Processing

Precision buffer preparation in controlled environments

Optimal pH stability and conductivity
Performance Testing

Comprehensive electrophoresis performance validation

Resolution and migration testing
Quality Systems

ISO 9001:2015 and GMP certified buffer production

Continuous improvement and validation
Storage Systems

Temperature-controlled storage with buffer preservation

Maintained electrophoretic performance

Market Applications & Performance Data

Comprehensive electrophoresis data demonstrating LR TBE Buffer effectiveness across diverse molecular biology applications with quantified performance metrics.

DNA Electrophoresis
Resolution: Sharp band separation 50-10000 bp pH Stability: 8.3 ± 0.1 maintained Conductivity: Optimized for 80-120V applications
Research Applications
Laboratories: 4000+ global research facilities Success Rate: 98% consistent gel performance Publications: 15000+ citing electrophoresis applications
Quality Control
Reproducibility: CV <3% between runs Stability: >1 year buffer performance Efficiency: 25% faster migration vs. TAE

DRAVYOM Competitive Advantages

Superior Resolution

Exceptional DNA separation performance with optimal buffering for precise molecular biology work

Reliable Supply

Guaranteed availability with molecular biology expertise and consistent buffer performance

Electrophoresis Expertise

Dedicated molecular biology team provides gel optimization and troubleshooting support

Quality Assurance

Comprehensive testing with electrophoretic performance validation and molecular biology documentation

Global Standards

International compliance with molecular biology and research standards for worldwide acceptance

Partnership Approach

Collaborative relationships with research institutions and custom buffer development services