YFP Yellow Fluorescent Protein
Biotechnology Premium
Fluorescent Protein

YFP Yellow Fluorescent Protein

High-quality YFP (Yellow Fluorescent Protein) for molecular biology research, protein labeling, and live cell imaging. Premium grade fluorescent protein manufactured for research applications and protein studies.

  • Yellow Fluorescence Emission
  • Protein Labeling Applications
  • Live Cell Imaging Compatible
  • Molecular Biology Reporter
  • Research Grade Purity
  • GFP Variant Technology

Technical Specifications

Protein Name: Yellow Fluorescent Protein (YFP)
Origin: Aequorea victoria (GFP variant)
Molecular Weight: 26.9 kDa
Excitation Maximum: 514 nm
Emission Maximum: 527 nm
Physical Form: Lyophilized powder
Purity: ≥95.0% (SDS-PAGE)
Quantum Yield: 0.61
Extinction Coefficient: 84,000 M⁻¹cm⁻¹
pH Stability: 6.0-8.0 (optimal)
Photostability: High resistance to photobleaching
Maturation Time: 40-60 minutes at 37°C
Storage Buffer: PBS, pH 7.4
Storage Temperature: -20°C, protect from light
Packaging: 10μg, 50μg, 100μg vials

Applications

Live Cell Imaging
Protein Labeling
Fluorescence Microscopy
Gene Expression Studies
Protein Trafficking
Flow Cytometry
Cell Biology Research
Molecular Biology
Plant Research

Industry-Specific Grades

DRAVYOM offers specialized YFP grades tailored for specific fluorescence and molecular biology requirements, ensuring optimal performance and consistent results across diverse research applications.

Research Grade
Purity: ≥95.0% Fluorescence: Stable Endotoxin: <1 EU/mg Application: General research, imaging
Imaging Grade
Purity: ≥98.0% Photostability: Enhanced Signal-to-Noise: Optimized Application: Live cell imaging, microscopy
Protein Fusion Grade
Purity: ≥99.0% Tag Compatibility: Verified Folding: Optimized Application: Protein tagging, localization
Flow Cytometry Grade
Purity: ≥97.0% Brightness: High Uniformity: Consistent Application: Flow cytometry, cell sorting

Quality Standards

DRAVYOM's YFP is manufactured under stringent quality control protocols, meeting international fluorescent protein standards for consistent performance and reliable fluorescence in research applications.

ISO 9001:2015 Certified Manufacturing
Fluorescent Protein Standards
≥95.0% SDS-PAGE Purity
Fluorescence Intensity Verified
Spectral Properties Confirmed
Freeze Storage Validated
Fluorescence Certificate Provided
Light-Protected Logistics

Advanced Chemical Properties & Performance

Yellow Fluorescent Protein exhibits exceptional photophysical properties essential for molecular imaging applications. Its optimized chromophore and high quantum yield ensure reliable performance in demanding fluorescence microscopy and cell biology studies.

Fluorescence Properties
Excitation Maximum: 514 nm
Emission Maximum: 527 nm
Quantum Yield: 0.61 (high brightness)
Extinction Coefficient: 83,400 M⁻¹cm⁻¹
Physical Properties
Protein Structure: β-barrel 238 amino acids
Molecular Weight: 26.9 kDa
Chromophore: p-Hydroxybenzylidene-imidazolinone
Maturation Time: 26 minutes at 37°C
Biochemical Performance
pH Stability: Stable pH 6-11
Photobleaching: t½ = 58 seconds (continuous)
Oligomerization: Weak dimer (A206K mutation)
Cellular Localization: Cytoplasmic, nuclear compatible
Purity Specifications
Protein Purity: ≥ 95% (SDS-PAGE)
Endotoxin Level: ≤ 1 EU/μg
DNA Contamination: ≤ 0.1%
Host Cell Proteins: ≤ 1%
Stability Properties
Shelf Life: 2 years (-80°C)
Thermal Stability: Stable up to 65°C
Freeze-Thaw: 5 cycles without loss
Working Solution: 1 week at 4°C

Performance Characteristics

Detailed performance metrics demonstrate YFP superiority in fluorescence applications with exceptional brightness, photostability, and reproducibility across diverse microscopy protocols and live cell imaging studies.

Brightness Performance

Relative brightness: 51 (vs. EGFP = 100)

Optimal for live cell imaging
Imaging Performance

Signal-to-noise ratio: 8.5:1

Excellent contrast in microscopy
Maturation Rate

Half-time: 26 minutes at 37°C

Rapid chromophore formation
Temperature Stability

Working range: 4-37°C optimal

Suitable for live cell work
Batch Reproducibility

Variation: ±3% between batches

Consistent fluorescence output
Photostability

t½ = 58 seconds continuous excitation

Reduced photobleaching

Safety Information

Generally safe recombinant protein for research use. Handle with standard laboratory precautions including safety goggles, gloves, and lab coat. Non-toxic and non-pathogenic. Avoid direct contact with skin and eyes. Store according to recommended conditions to maintain protein integrity.

Research Safe
Recombinant Protein
Standard Lab Precautions

Storage & Handling

Store in original vials at -80°C for long-term storage or -20°C for short-term use. Avoid repeated freeze-thaw cycles. Keep working solutions at 4°C and use within one week. Protect from direct light during handling to prevent photobleaching.

Ultra-low storage (-80°C)
Avoid freeze-thaw cycles
Original vials recommended
Protect from light

Chemical Mechanisms & Reaction Pathways

YFP exhibits fluorescent protein properties through its β-barrel structure and chromophore formation, enabling precise molecular visualization with predictable excitation/emission characteristics and quantitative fluorescence measurements.

Chromophore Formation

Autocatalytic cyclization creates yellow fluorescent chromophore

Essential for fluorescence microscopy applications
Protein Folding

Proper β-barrel formation protects internal chromophore

Critical for maintaining fluorescence stability
Excitation/Emission

Optimal excitation at 514nm with emission at 527nm

Enables specific fluorescence detection
Protein Tagging

Fusion protein formation for cellular localization studies

Key for live cell imaging applications

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global biotechnology laboratory access with complete documentation packages supporting international standards and molecular biology method validations.

Research Grade

Ultra-pure specifications for molecular biology applications

ISO 6353 Standard

International standard for reagent specifications and testing

USP Grade Available

United States Pharmacopeia specifications for pharmaceutical research

REACH Registration

European Union chemical regulation compliance

Method Validation

Supported molecular biology method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's molecular biology team provides comprehensive method support, troubleshooting assistance, and biotechnology services to optimize YFP performance in your specific fluorescence applications.

Method Development
  • Fluorescence protocol optimization support
  • Expression and purification guidance
  • Imaging parameter validation
  • Custom fluorescent protein solutions
Analytical Services
  • Certificate of Analysis verification
  • Fluorescence intensity testing
  • Protein purity analysis
  • Photostability validation
Technical Support
  • Imaging troubleshooting consultation
  • Storage and handling guidance
  • Fluorescence optimization
  • Laboratory best practices
Supply Solutions
  • Consistent lot scheduling
  • Emergency supply arrangements
  • Light-protected packaging
  • Ultra-low temperature distribution

Environmental Impact & Sustainability

Our YFP production emphasizes environmental responsibility through sustainable manufacturing practices, waste minimization, and comprehensive environmental impact management for molecular biology laboratory operations.

Waste Minimization

Optimized production with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Clean Production

Energy-efficient expression with emission controls

Safe Disposal

Comprehensive guidance for laboratory waste management

ISO 14001

Environmental management system certified production

Container Recycling

Container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art biotechnology facility employs advanced protein expression technology and continuous monitoring systems to ensure consistent YFP quality and performance across all production batches.

Production Process

Advanced expression and purification in controlled sterile environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive testing including fluorescence intensity, purity, and activity

Fluorescence spectroscopy verification
Quality Systems

ISO 9001:2015 quality management with molecular biology laboratory accreditation

Continuous improvement and bioprocess validation
Packaging Control

Light-protected vials with controlled atmosphere packaging

Complete photobleaching protection and stability maintenance

Market Applications & Performance Data

Comprehensive molecular biology laboratory data demonstrating YFP effectiveness across diverse applications with quantified performance metrics and fluorescence method validations.

Fluorescence Microscopy Labs
Fluorescence Intensity: >95% quantum yield retention Purity: >98% by SDS-PAGE analysis Photostability: >90% intensity after 1 hour illumination
Research Institutions
Publication Support: 1500+ cited protocols Reproducibility: >99% inter-lab agreement Innovation: Custom fluorescent protein variants
Biotech Companies
Reliability: 99.9% lot consistency Audit Success: 100% regulatory compliance Efficiency: 45% improved imaging workflows

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds molecular biology specifications with ultra-high fluorescence intensity and exceptional photostability

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Protein Engineering Expertise

Dedicated fluorescent protein team provides imaging development and troubleshooting support

Quality Assurance

Traceable certificates with comprehensive fluorescence testing and stability validation support

Global Standards

International compliance with molecular biology and research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with fluorescence laboratories and custom protein development services