X-Gal (Chromogenic Substrate)
High-purity X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) essential for molecular biology applications. Provides reliable blue-white screening for gene expression studies, cloning procedures, and β-galactosidase activity detection.
- High-Purity Chromogenic Substrate
- Blue-White Screening
- Gene Expression Studies
- β-Galactosidase Detection
- Cloning Applications
- Research Validated
Technical Specifications
Applications
Industry-Specific Grades
DRAVYOM offers specialized X-Gal grades tailored for specific molecular biology and biotechnology requirements, ensuring optimal performance and consistent results across diverse applications.
Research Grade
Molecular Biology Grade
Chromogenic Grade
Plant Transformation Grade
Quality Standards
DRAVYOM's X-Gal is manufactured under stringent quality control protocols, meeting international molecular biology standards for consistent blue-white screening and reliable gene expression detection.
Advanced Chemical Properties & Performance
X-Gal exhibits exceptional chromogenic properties essential for molecular biology applications. Its specific substrate activity and high purity ensure reliable performance in demanding gene expression studies and screening procedures.
Chromogenic Properties
Physical Properties
Enzymatic Performance
Purity Specifications
Stability Properties
Performance Characteristics
Detailed performance metrics demonstrate X-Gal superiority in gene expression applications with exceptional chromogenic response, specificity, and reproducibility across diverse molecular biology protocols and screening procedures.
Detection Sensitivity
LOD: 0.1 μg β-galactosidase enzyme
Ultra-sensitive screening capabilityGene Expression Correlation
Linear response: R² = 0.98
Quantitative gene expression analysisColor Development
Blue color: 2-24h development time
Visible result confirmationTemperature Stability
Working range: -20°C storage stable
Long-term reagent stabilityBatch Reproducibility
Variation: ±5% between batches
Consistent screening resultsStability Performance
3 years under proper storage conditions
Extended substrate integritySafety Information
Handle with standard molecular biology precautions including safety goggles, gloves, and lab coat. Avoid inhalation of powder and skin contact. Contains halogenated aromatic compound - use in well-ventilated areas. May cause irritation to eyes, skin, and respiratory tract. Dispose according to local regulations.
Storage & Handling
Store in original amber containers at -20°C in a dry, dark place. Keep containers tightly closed and use desiccant to prevent moisture absorption. Prepare working solutions fresh or store at 4°C for short-term use. Protect from light during handling and use.
Chemical Mechanisms & Reaction Pathways
X-Gal exhibits chromogenic substrate properties through its galactopyranoside structure and indole functionality, enabling precise β-galactosidase detection with predictable enzymatic cleavage and quantitative blue color development.
Enzymatic Cleavage
β-galactosidase cleaves galactose moiety to release chromophore
Essential for blue-white screening protocolsIndole Formation
Released indoxyl undergoes oxidation to form blue indigo
Critical for visual colony identificationColor Development
Spontaneous dimerization produces intense blue coloration
Enables rapid screening applicationsGene Expression
Reports lacZ gene expression and promoter activity
Key for molecular cloning studiesRegulatory Compliance & Documentation
Comprehensive regulatory compliance ensures global biotechnology laboratory access with complete documentation packages supporting international standards and molecular biology method validations.
Molecular Biology Grade
Ultra-pure specifications for molecular biology applications
ISO 6353 Standard
International standard for reagent specifications and testing
USP Grade Available
United States Pharmacopeia specifications for pharmaceutical research
REACH Registration
European Union chemical regulation compliance
Method Validation
Supported molecular biology method validation documentation
SDS Documentation
Multi-language Safety Data Sheets (16 sections, GHS compliant)
Technical Support & Value-Added Services
DRAVYOM's molecular biology team provides comprehensive method support, troubleshooting assistance, and biotechnology services to optimize X-Gal performance in your specific cloning and screening applications.
Method Development
- Screening protocol optimization support
- Concentration and timing guidance
- Color development validation
- Custom screening solutions
Analytical Services
- Certificate of Analysis verification
- Purity and activity testing
- Enzymatic assay validation
- Stability analysis
Technical Support
- Cloning troubleshooting consultation
- Storage and preparation guidance
- Screening optimization
- Laboratory best practices
Supply Solutions
- Consistent lot scheduling
- Emergency supply arrangements
- Light-protected packaging
- Frozen distribution
Environmental Impact & Sustainability
Our X-Gal production emphasizes environmental responsibility through sustainable manufacturing practices, waste minimization, and comprehensive environmental impact management for molecular biology laboratory operations.
Waste Minimization
Optimized production with minimal waste generation
Water Treatment
Advanced wastewater treatment and recycling systems
Clean Production
Energy-efficient synthesis with emission controls
Safe Disposal
Comprehensive guidance for laboratory waste management
ISO 14001
Environmental management system certified production
Container Recycling
Container return and recycling programs
Manufacturing Excellence & Quality Control
DRAVYOM's state-of-the-art biotechnology facility employs advanced purification technology and continuous monitoring systems to ensure consistent X-Gal quality and performance across all production batches.
Production Process
Advanced crystallization and purification in controlled light-protected environment
Multi-stage purification for molecular biology grade qualityQuality Testing
Comprehensive testing including purity, enzymatic activity, and color development
β-galactosidase assay verificationQuality Systems
ISO 9001:2015 quality management with molecular biology laboratory accreditation
Continuous improvement and bioprocess validationPackaging Control
Amber containers with desiccant and controlled atmosphere packaging
Complete light protection and moisture controlMarket Applications & Performance Data
Comprehensive molecular biology laboratory data demonstrating X-Gal effectiveness across diverse applications with quantified performance metrics and cloning method validations.
Molecular Biology Labs
Research Institutions
Biotech Companies
DRAVYOM Competitive Advantages
Superior Purity
Consistently exceeds molecular biology specifications with ultra-high enzymatic sensitivity and exceptional color development
Reliable Supply
Guaranteed availability with strategic inventory management and molecular biology-grade production scheduling
Molecular Biology Expertise
Dedicated cloning team provides screening development and troubleshooting support
Quality Assurance
Traceable certificates with comprehensive enzymatic testing and stability validation support
Global Standards
International compliance with molecular biology and USP specifications for worldwide acceptance
Partnership Approach
Collaborative relationships with molecular biology laboratories and custom screening development services