Tween 20 Detergent
Biotechnology Premium
Non-ionic Detergent

Tween 20 Detergent

High-quality Tween 20 (Polysorbate 20) for molecular biology, protein purification, and biochemical applications. Premium grade non-ionic detergent manufactured for research use and protein studies.

  • Non-ionic Surfactant
  • Protein Purification Compatible
  • Cell Lysis Applications
  • Western Blot Blocking
  • Research Grade Quality
  • Molecular Biology Standard
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Technical Specifications

Chemical Name: Polyoxyethylene sorbitan monolaurate
CAS Number: 9005-64-5
Molecular Weight: 1,227.54 g/mol (average)
Physical Form: Clear to pale yellow viscous liquid
Purity: ≥98.0% (Molecular Biology Grade)
HLB Value: 16.7
CMC (Critical Micelle Concentration): 0.06 mM
Density (25°C): 1.095 g/cm³
Viscosity (25°C): 400 cP
pH (5% solution): 5.0-7.0
Solubility: Soluble in water and ethanol
Storage Temperature: 2-8°C
Packaging Options: 100ml, 500ml, 1L amber glass bottles

Applications

Western Blot Blocking
Protein Purification
Cell Lysis Buffer
ELISA Wash Buffer
Membrane Protein Solubilization
Immunohistochemistry
Protein Crystallization
Biochemical Assays
Plant Protein Extraction
Neuroscience Research
Cardiac Protein Studies
Pharmaceutical Research

Industry-Specific Grades

DRAVYOM offers specialized Tween 20 grades tailored for specific biotechnology and research applications, ensuring optimal performance and regulatory compliance across diverse protein research and biochemical applications.

Molecular Biology Grade
Purity: ≥98.0% Endotoxin: ≤ 0.1 EU/ml DNase/RNase: None detected Application: Western blot, protein research
Research Grade
Purity: ≥97.0% Heavy Metals: ≤ 10 ppm Peroxide Value: ≤ 5.0 Application: General biochemical research
Ultra-Pure Grade
Purity: ≥99.0% Protein Content: ≤ 0.1% Fatty Acids: ≤ 2.0% Application: Protein crystallization
Cell Culture Grade
Purity: ≥98.5% Sterility: Sterile filtered Mycoplasma: Tested negative Application: Cell culture applications

Quality Standards

DRAVYOM's Tween 20 is manufactured under stringent quality control protocols, meeting international biotechnology standards including USP, EP, and research-grade specifications. Our molecular biology-grade production ensures consistent performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
USP/EP Grade Specifications
≥98.0% Molecular Biology Purity
Advanced Analytical Testing
Ultra-Low Endotoxin Levels
Batch-to-Batch Consistency
Traceable Certificate of Analysis
Temperature-Controlled Logistics

Advanced Chemical Properties & Performance

Biotechnology-grade Tween 20 exhibits exceptional properties essential for cell membrane permeabilization, protein solubilization, and immunoassay applications. Its ultra-pure composition and controlled fatty acid content ensure reliable performance in demanding biochemical applications.

Chemical Properties
Chemical Name: Polyoxyethylene sorbitan monolaurate
Average Molecular Weight: 1228 g/mol
CAS Number: 9005-64-5
Surfactant Type: Non-ionic detergent
Physical Properties
Appearance: Clear to slightly amber viscous liquid
Cloud Point: 76-78°C (5% aqueous)
Density: 1.1 g/cm³ at 25°C
Viscosity: 400 cP at 25°C
Surfactant Properties
CMC (25°C): 0.059 mM
HLB Value: 16.7
Surface Tension: 40.6 mN/m at CMC
Aggregation Number: 60
Purity Specifications
Purity: ≥ 99%
Water Content: ≤ 3%
Peroxide Value: ≤ 10 meq O₂/kg
Heavy Metals: ≤ 10 ppm
Stability Properties
Shelf Life: 3 years at room temperature
pH Stability: Stable pH 3-11
Temperature Stability: Stable to 76°C
Autoclave Compatible: Yes (dilute solutions)

Performance Characteristics

Detailed performance metrics demonstrate Biotechnology Grade Tween 20 superiority in immunoassays with exceptional membrane permeabilization, protein solubilization efficiency, and reproducibility across diverse analytical applications.

Immunoassay Performance

Background reduction: >90% in ELISA

Excellent blocking agent
Membrane Permeabilization

Cell permeability: Gentle, reversible

Mild detergent action
Protein Solubilization

Membrane proteins: Minimal denaturation

Preserves native structure
Temperature Performance

Working range: 4-76°C

Wide temperature compatibility
Batch Consistency

CMC variation: ±3% between lots

Consistent detergent activity
Wash Efficiency

Buffer washing: Complete removal

Easy rinsing

Safety Information

Generally safe for laboratory use with minimal hazards. May cause mild eye and skin irritation upon direct contact. Use standard laboratory safety practices including safety goggles and gloves. May cause slippery surfaces when spilled.

Eye Irritant
Skin Irritant
Slippery When Spilled

Storage & Handling

Store in cool, dry place away from heat and light to prevent oxidation. Keep containers tightly sealed to prevent contamination. Handle with standard laboratory protective equipment. Clean spills immediately as surfactants create slippery surfaces.

Store at 15-25°C
Protect from light
Use protective gloves
Clean spills immediately

Chemical Mechanisms & Reaction Pathways

Tween 20 exhibits non-ionic surfactant properties through its polyoxyethylene chains and fatty acid core, enabling precise protein solubilization with predictable detergent action and quantitative membrane protein extraction.

Protein Solubilization

Gentle solubilization of membrane proteins without denaturation

Essential for protein purification procedures
Micelle Formation

Forms protective micelles around hydrophobic protein regions

Critical for maintaining protein structure
Blocking Agent

Prevents non-specific protein binding in immunoassays

Enables specific antibody interactions
Cell Permeabilization

Mild membrane permeabilization for cell-based assays

Key for maintaining cell viability

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global biotechnology laboratory access with complete documentation packages supporting international standards and protein research method validations.

Molecular Biology Grade

Ultra-pure specifications for molecular biology applications

ISO 6353 Standard

International standard for reagent specifications and testing

USP Grade Available

United States Pharmacopeia specifications for pharmaceutical research

REACH Registration

European Union chemical regulation compliance

Method Validation

Supported protein research method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's protein biochemistry team provides comprehensive method support, troubleshooting assistance, and biotechnology services to optimize Tween 20 performance in your specific protein research applications.

Method Development
  • Protein purification optimization support
  • Concentration and buffer guidance
  • Extraction efficiency validation
  • Custom detergent formulations
Analytical Services
  • Certificate of Analysis verification
  • Peroxide and contamination testing
  • Protein recovery analysis
  • CMC determination
Technical Support
  • Protein purification troubleshooting
  • Storage and stability guidance
  • Immunoassay optimization
  • Laboratory best practices
Supply Solutions
  • Consistent lot scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Temperature-controlled distribution

Environmental Impact & Sustainability

Our Tween 20 production emphasizes environmental responsibility through sustainable manufacturing practices, waste minimization, and comprehensive environmental impact management for protein research laboratory operations.

Waste Minimization

Optimized production with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Clean Production

Energy-efficient synthesis with emission controls

Safe Disposal

Comprehensive guidance for laboratory waste management

ISO 14001

Environmental management system certified production

Container Recycling

Container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art biotechnology facility employs advanced purification technology and continuous monitoring systems to ensure consistent Tween 20 quality and performance across all production batches.

Production Process

Advanced distillation and purification in controlled sterile environment

Multi-stage purification for molecular biology grade quality
Quality Testing

Comprehensive testing including peroxide, pH, and protein compatibility

CMC determination and purity verification
Quality Systems

ISO 9001:2015 quality management with protein research laboratory accreditation

Continuous improvement and bioprocess validation
Packaging Control

Sterile containers with controlled atmosphere packaging

Contamination prevention and oxidation control

Market Applications & Performance Data

Comprehensive protein research laboratory data demonstrating Tween 20 effectiveness across diverse applications with quantified performance metrics and molecular biology method validations.

Protein Research Labs
Protein Recovery: >85% yield in membrane protein purification Purity: >99% by HPLC analysis Activity Retention: >95% enzyme activity preservation
Research Institutions
Publication Support: 1200+ cited protocols Reproducibility: >99% inter-lab agreement Innovation: Custom detergent formulations
Biotech Companies
Reliability: 99.9% lot consistency Audit Success: 100% regulatory compliance Efficiency: 35% improved purification yields

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds molecular biology specifications with ultra-low peroxides and exceptional protein compatibility

Reliable Supply

Guaranteed availability with strategic inventory management and molecular biology-grade production scheduling

Protein Research Expertise

Dedicated protein biochemistry team provides purification development and troubleshooting support

Quality Assurance

Traceable certificates with comprehensive protein compatibility testing and validation support

Global Standards

International compliance with molecular biology and USP specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with protein research laboratories and custom detergent development services