Triton X-100 Detergent
Biotechnology Premium
Cell Lysis Detergent

Triton X-100 Detergent

High-quality Triton X-100 for cell lysis, protein extraction, and membrane permeabilization. Premium grade non-ionic detergent manufactured for molecular biology applications and protein research.

  • Cell Lysis & Permeabilization
  • Protein Extraction Compatible
  • Membrane Solubilization
  • Non-ionic Surfactant
  • Research Grade Quality
  • Molecular Biology Standard
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Technical Specifications

Chemical Name: Triton X-100
Chemical Type: Octylphenol ethoxylate
Molecular Formula: C₁₄H₂₂O(C₂H₄O)ₙ
Average Molecular Weight: 625 g/mol
CAS Number: 9002-93-1
Physical Form: Clear viscous liquid
CMC: 0.24 mM (0.015%)
HLB Value: 13.5
Working Concentration: 0.1-1.0%
Cloud Point: 64°C (1% solution)
Storage Temperature: Room temperature
Packaging Options: 100mL, 500mL, 1L, 4L

Applications

Cell Lysis
Protein Extraction
Membrane Solubilization
Western Blotting
Immunohistochemistry
Educational Research
Cell Permeabilization
Enzyme Assays
Plant Cell Studies
DNA/RNA Isolation
Protein Purification
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized Triton X-100 grades tailored for specific cell lysis and protein extraction applications, ensuring optimal surfactant performance and regulatory compliance across diverse biotechnology requirements.

Research Grade
Purity: ≥98% CMC: 0.24 mM Heavy metals: <20 ppm Application: General research
Molecular Biology Grade
Purity: ≥99% DNase/RNase: None detected Protease: None detected Application: Protein work
Ultra-Pure Grade
Purity: ≥99.5% Heavy metals: <5 ppm Peroxides: <0.5% Application: Sensitive analysis
Cell Culture Grade
Purity: ≥99% Endotoxin: <0.1 EU/mL Sterility: Assured Application: Cell culture

Quality Standards

DRAVYOM's Triton X-100 is manufactured under stringent quality control protocols, meeting international surfactant standards including molecular biology specifications and cell culture requirements. Our detergent production ensures consistent performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Molecular Biology Grade Specifications
≥98% Purity Verified
CMC Testing
Surfactant Analysis
Enzyme Activity Testing
Certificate of Analysis
Temperature-Controlled Storage

Advanced Chemical Properties & Performance

Biotechnology-grade Triton X-100 exhibits exceptional properties essential for membrane solubilization, protein extraction, and cell lysis applications. Its ultra-pure composition and controlled polyethylene glycol content ensure reliable performance in demanding biochemical applications.

Chemical Properties
Chemical Name: 4-(1,1,3,3-tetramethylbutyl)phenyl-polyethylene glycol
Average Molecular Weight: 647 g/mol (n~9.5)
CAS Number: 9002-93-1
Surfactant Type: Non-ionic detergent
Physical Properties
Appearance: Clear to slightly hazy viscous liquid
Cloud Point: 65°C (1% aqueous)
Density: 1.07 g/cm³ at 25°C
Viscosity: 240 cP at 25°C
Surfactant Properties
CMC (25°C): 0.22-0.24 mM
HLB Value: 13.5
Surface Tension: 30.9 mN/m at CMC
Aggregation Number: 140
Purity Specifications
Purity: ≥ 98%
Water Content: ≤ 2%
Peroxide Value: ≤ 10 meq O₂/kg
Heavy Metals: ≤ 5 ppm
Stability Properties
Shelf Life: 3 years at room temperature
pH Stability: Stable pH 2-12
Temperature Stability: Stable to 65°C
Oxidation Sensitivity: Moderate (store cool)

Performance Characteristics

Detailed performance metrics demonstrate Biotechnology Grade Triton X-100 superiority in membrane biology with exceptional solubilization power, protein extraction efficiency, and reproducibility across diverse biochemical applications.

Membrane Solubilization

Lipid extraction: >90% efficiency

Effective membrane disruption
Protein Compatibility

Native structure: Minimal denaturation

Gentle protein extraction
Cell Lysis Efficiency

Cell breakage: Complete lysis

Effective cellular disruption
Temperature Performance

Working range: 4-65°C

Wide temperature compatibility
Batch Consistency

CMC variation: ±5% between lots

Consistent detergent activity
Solubilization Rate

Membrane dissolution: Rapid kinetics

Fast solubilization

Safety Information

Mildly irritating to eyes and skin. Can cause respiratory irritation if inhaled. Non-ionic surfactant with low toxicity but avoid direct contact and inhalation. Use in well-ventilated areas with appropriate protective equipment. May cause slippery surfaces.

Eye Irritant
Skin Irritant
Slippery When Spilled

Storage & Handling

Store in cool, dry place away from heat and light to prevent oxidation. Keep containers tightly sealed to prevent contamination. Handle with standard laboratory protective equipment. Clean spills immediately as surfactants create slippery surfaces.

Store at 15-25°C
Protect from light
Use protective gloves
Clean spills immediately

Chemical Mechanisms & Reaction Pathways

Triton X-100 exhibits non-ionic surfactant properties through its polyethylene oxide chain and hydrophobic aromatic core, enabling precise membrane solubilization with predictable detergent action and quantitative protein extraction.

Membrane Solubilization

Disrupts lipid bilayers through hydrophobic insertion

Essential for protein extraction procedures
Protein Solubilization

Forms mixed micelles with membrane proteins

Critical for maintaining protein integrity
Critical Micelle Formation

Self-assembles into micelles at CMC (0.24 mM)

Enables controlled detergent action
Cell Permeabilization

Creates membrane pores for intracellular access

Key for cell lysis applications

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global biotechnology laboratory access with complete documentation packages supporting international standards and cell biology method validations.

Cell Culture Grade

Ultra-pure specifications for cell culture applications

ISO 6353 Standard

International standard for reagent specifications and testing

USP Grade Available

United States Pharmacopeia specifications for pharmaceutical research

REACH Registration

European Union chemical regulation compliance

Method Validation

Supported cell biology method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's cell biology team provides comprehensive method support, troubleshooting assistance, and biotechnology services to optimize Triton X-100 performance in your specific cellular applications.

Method Development
  • Lysis protocol optimization support
  • Concentration and timing guidance
  • Extraction efficiency validation
  • Custom lysis buffer formulations
Analytical Services
  • Certificate of Analysis verification
  • Endotoxin and contamination testing
  • Protein recovery analysis
  • Cytotoxicity validation
Technical Support
  • Protocol troubleshooting consultation
  • Storage and stability guidance
  • Cell culture training
  • Laboratory best practices
Supply Solutions
  • Consistent lot scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Temperature-controlled distribution

Environmental Impact & Sustainability

Our Triton X-100 production emphasizes environmental responsibility through sustainable manufacturing practices, waste minimization, and comprehensive environmental impact management for cell biology laboratory operations.

Waste Minimization

Optimized production with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Clean Production

Energy-efficient synthesis with emission controls

Safe Disposal

Comprehensive guidance for laboratory waste management

ISO 14001

Environmental management system certified production

Container Recycling

Container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art biotechnology facility employs advanced purification technology and continuous monitoring systems to ensure consistent Triton X-100 quality and performance across all production batches.

Production Process

Advanced purification and distillation in controlled sterile environment

Multi-stage purification for cell culture grade quality
Quality Testing

Comprehensive testing including endotoxin, peroxide, and cytotoxicity analysis

Cell culture compatibility verification
Quality Systems

ISO 9001:2015 quality management with cell biology laboratory accreditation

Continuous improvement and bioprocess validation
Packaging Control

Sterile containers with controlled atmosphere packaging

Contamination prevention and stability maintenance

Market Applications & Performance Data

Comprehensive cell biology laboratory data demonstrating Triton X-100 effectiveness across diverse applications with quantified performance metrics and cell culture method validations.

Cell Biology Labs
Protein Recovery: >90% yield in membrane protein extraction Purity: >99% by HPLC analysis Cell Viability: Controlled permeabilization without cytotoxicity
Research Institutions
Publication Support: 800+ cited protocols Reproducibility: >99% inter-lab agreement Innovation: Custom detergent formulations
Biotech Companies
Reliability: 99.9% lot consistency Audit Success: 100% regulatory compliance Efficiency: 30% improved extraction yields

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds cell culture specifications with ultra-low contaminants and exceptional biocompatibility

Reliable Supply

Guaranteed availability with strategic inventory management and cell culture-grade production scheduling

Cell Biology Expertise

Dedicated cell culture team provides protocol development and troubleshooting support

Quality Assurance

Traceable certificates with comprehensive biological testing and validation support

Global Standards

International compliance with cell culture and USP specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with cell biology laboratories and custom formulation services