Tris-HCl Buffer
Biotechnology Buffer
Biotechnology Reagents

Tris-HCl (Tris Hydroxyl Methyl Hydrochloride)

High-purity Tris-HCl buffer component essential for biotechnology applications. Provides reliable pH buffering capacity for molecular biology procedures, biochemical assays, and research applications requiring precise pH control.

  • High-Purity Buffer Component
  • Reliable pH Buffering
  • Molecular Biology Grade
  • Biochemical Research Compatible
  • Consistent Performance
  • Laboratory Validated
Request Quote Technical Support

Technical Specifications

Chemical Name: Tris(hydroxymethyl)aminomethane HCl
Molecular Formula: C₄H₁₁NO₃·HCl
Molecular Weight: 157.60 g/mol
CAS Number: 1185-53-1
Assay: ≥99.0% (ACS grade)
Physical Form: White crystalline powder
pH (0.05 M solution): 4.3-5.0
pKa: 8.1 (at 25°C)
Solubility: Very soluble in water
Loss on Drying: ≤0.3%
Storage Temperature: Room temperature (dry place)
Packaging Options: 25g, 100g, 500g, 1kg

Applications

Buffer Preparation
Protein Studies
Enzyme Assays
DNA/RNA Work
Electrophoresis
Educational Research
Cell Culture
PCR Applications
Plant Biochemistry
pH Control
Protein Purification
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized Tris-HCl grades tailored for specific buffering and molecular biology applications, ensuring optimal pH control and regulatory compliance across diverse biotechnology requirements.

ACS Grade
Assay: ≥99.0% pH: 4.3-5.0 Heavy metals: <10 ppm Application: General research
Molecular Biology Grade
Assay: ≥99.9% DNase/RNase: None detected Endotoxin: <0.1 EU/g Application: Molecular biology
Ultra-Pure Grade
Assay: ≥99.95% Heavy metals: <1 ppm Protease: None detected Application: Protein work
Cell Culture Grade
Assay: ≥99.5% (USP) Sterility: Assured Pyrogen: Free Application: Cell culture

Quality Standards

DRAVYOM's Tris-HCl is manufactured under stringent quality control protocols, meeting international buffer standards including ACS specifications and molecular biology requirements. Our buffer production ensures consistent pH performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
ACS Grade Specifications
≥99.0% Assay Verified
pH Testing
Buffer Capacity Analysis
Nuclease Testing
Certificate of Analysis
Moisture-Controlled Storage

Advanced Chemical Properties & Performance

Biotechnology-grade Tris-HCl exhibits exceptional properties essential for biological buffering, protein biochemistry, and molecular biology applications. Its ultra-pure composition and precise pH control ensure reliable performance in demanding research applications.

Chemical Properties
Molecular Formula: C₄H₁₁NO₃ · HCl
Molecular Weight: 157.60 g/mol
CAS Number: 1185-53-1
Buffer Type: Good's buffer (biological)
Physical Properties
Appearance: White crystalline powder
Melting Point: 168-172°C
Solubility (water): >500 g/L at 25°C
pH (0.05M solution): 4.3-4.7
Buffer Properties
pKa (25°C): 8.06
Buffer Range: pH 7.0-9.0
Temperature Coefficient: -0.028 pH units/°C
Ionic Strength Effect: Minimal pH variation
Purity Specifications
Purity: ≥ 99.8%
Heavy Metals: ≤ 5 ppm
DNase Activity: None detected
RNase Activity: None detected
Stability Properties
Shelf Life: 5 years in dry conditions
Solution Stability: Stable at 4°C for months
Autoclave Stable: Yes (121°C, 15 min)
pH Drift: Minimal over time

Performance Characteristics

Detailed performance metrics demonstrate Biotechnology Grade Tris-HCl superiority in biological buffering with exceptional pH stability, protein compatibility, and reproducibility across diverse biochemical applications.

Buffer Capacity

pH maintenance: ±0.1 pH units

Excellent buffering power
Protein Compatibility

Enzyme activity: >95% retention

Biocompatible buffer
Temperature Stability

Working range: 4-37°C

Stable across biological temps
pH Precision

pH accuracy: ±0.05 pH units

Precise pH control
Batch Consistency

pH variation: ±0.02 pH between lots

Reliable buffering
Long-term Stability

Solution storage: Months at 4°C

Extended usability

Safety Information

Generally regarded as safe for laboratory use with minimal hazards. May cause mild eye and skin irritation upon direct contact. Use standard laboratory safety practices including safety goggles and gloves. Avoid inhalation of powder.

Eye Irritant
Skin Irritant
Generally Safe

Storage & Handling

Store in cool, dry place in tightly sealed containers away from moisture. Handle with standard laboratory protective equipment including safety goggles and gloves. Solutions can be stored at 4°C for extended periods. Autoclave sterilizable for sterile applications.

Store at room temperature
Keep dry (hygroscopic)
Autoclave compatible
Solutions stable at 4°C

Chemical Mechanisms & Reaction Pathways

Tris-HCl exhibits excellent buffering capacity through its amino group protonation-deprotonation equilibrium, enabling precise pH control in biological systems with predictable buffering pathways and quantitative acid-base behavior.

Buffer Chemistry

Primary amine protonation equilibrium for pH buffering

Essential for biological system pH control
Metal Coordination

Coordination with divalent metal ions in enzymatic systems

Critical for enzyme stabilization
Protein Interactions

Minimal protein binding for biological compatibility

Enables protein purification applications
Ionic Strength

Contributes to solution ionic strength and conductivity

Important for electrophoretic separations

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global biotechnology laboratory access with complete documentation packages supporting international standards and molecular biology method validations.

Molecular Biology Grade

Ultra-pure specifications for molecular biology applications

ISO 6353 Standard

International standard for reagent specifications and testing

USP Grade Available

United States Pharmacopeia specifications for pharmaceutical research

REACH Registration

European Union chemical regulation compliance

Method Validation

Supported molecular biology method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's molecular biology team provides comprehensive method support, troubleshooting assistance, and biotechnology services to optimize Tris-HCl performance in your specific biological applications.

Method Development
  • Buffer system optimization support
  • pH and concentration guidance
  • Protocol development and validation
  • Custom buffer formulations
Analytical Services
  • Certificate of Analysis verification
  • Endotoxin and nuclease testing
  • Metal contamination analysis
  • Biocompatibility validation
Technical Support
  • Protocol troubleshooting consultation
  • Storage and stability guidance
  • Sterile technique training
  • Laboratory best practices
Supply Solutions
  • Consistent lot scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Cold chain distribution

Environmental Impact & Sustainability

Our Tris-HCl production emphasizes environmental responsibility through sustainable manufacturing practices, waste minimization, and comprehensive environmental impact management for biotechnology laboratory operations.

Waste Minimization

Optimized production with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Clean Production

Energy-efficient synthesis with emission controls

Safe Disposal

Comprehensive guidance for laboratory waste management

ISO 14001

Environmental management system certified production

Container Recycling

Container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art biotechnology facility employs advanced purification technology and continuous monitoring systems to ensure consistent Tris-HCl quality and performance across all production batches.

Production Process

Advanced crystallization and purification in controlled sterile environment

Multi-stage purification for molecular biology grade quality
Quality Testing

Comprehensive testing including endotoxin, nuclease, and metal contamination

Biocompatibility verification and sterility testing
Quality Systems

ISO 9001:2015 quality management with biotechnology laboratory accreditation

Continuous improvement and bioprocess validation
Packaging Control

Sterile containers with controlled atmosphere packaging

Contamination prevention and stability maintenance

Market Applications & Performance Data

Comprehensive biotechnology laboratory data demonstrating Tris-HCl effectiveness across diverse applications with quantified performance metrics and molecular biology method validations.

Molecular Biology Labs
Buffer Stability: >99% pH maintenance over 12 months Purity: >99.9% by HPLC analysis Biocompatibility: 100% enzyme activity retention
Research Institutions
Publication Support: 1000+ cited protocols Reproducibility: >99% inter-lab agreement Innovation: Custom buffer development
Biotech Companies
Reliability: 99.9% lot consistency Audit Success: 100% regulatory compliance Efficiency: 20% faster assay development

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds molecular biology specifications with ultra-low contaminants and exceptional biocompatibility

Reliable Supply

Guaranteed availability with strategic inventory management and molecular biology-grade production scheduling

Biotechnology Expertise

Dedicated molecular biology team provides buffer development and troubleshooting support

Quality Assurance

Traceable certificates with comprehensive biological testing and validation support

Global Standards

International compliance with molecular biology and USP specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with biotechnology laboratories and custom buffer development services