TAE/TBE Electrophoresis Buffers
Biotechnology Buffer
Electrophoresis Buffers

TAE/TBE Buffers (for Electrophoresis)

High-quality TAE (Tris-acetate-EDTA) and TBE (Tris-borate-EDTA) buffer systems essential for gel electrophoresis applications. Provides excellent conductivity, pH stability, and consistent performance for DNA/RNA separation and molecular biology procedures.

  • High-Quality Buffer Systems
  • Excellent Conductivity
  • pH Stability
  • DNA/RNA Compatible
  • Consistent Performance
  • Molecular Biology Grade
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Technical Specifications

Buffer System: TAE/TBE Buffer Kit
TAE Components: Tris-acetate-EDTA
TBE Components: Tris-borate-EDTA
pH (1X TAE): 8.3 ± 0.1
pH (1X TBE): 8.3 ± 0.1
Concentration: 50X stock solution
Buffer Capacity: 40 mM Tris-acetate
EDTA Concentration: 1 mM
Conductivity: Low ionic strength
Stability: 2 years at room temperature
Storage Temperature: Room temperature
Packaging Options: 500mL, 1L, 5L (50X stock)

Applications

DNA Electrophoresis
RNA Electrophoresis
Agarose Gel Running
PCR Product Analysis
DNA Fragment Size
Educational Research
Molecular Biology
Gel Purification
Plant DNA Analysis
Nucleic Acid Research
Quality Control
Diagnostics

Industry-Specific Grades

DRAVYOM offers specialized TAE/TBE buffer grades tailored for specific electrophoresis applications, ensuring optimal separation performance and regulatory compliance across diverse molecular biology requirements.

Research Grade
pH: 8.3 ± 0.1 Resolution: Standard Heavy metals: <20 ppm Application: General research
Molecular Biology Grade
pH: 8.3 ± 0.05 DNase/RNase: None detected Endotoxin: <0.1 EU/mL Application: Sensitive work
Ultra-Pure Grade
pH: 8.3 ± 0.02 Heavy metals: <5 ppm Nucleases: None detected Application: High resolution
Diagnostic Grade
pH: 8.3 ± 0.1 (USP) GMP: Compliant Validation: Clinical Application: Diagnostics

Quality Standards

DRAVYOM's TAE/TBE Buffers are manufactured under stringent quality control protocols, meeting international electrophoresis standards including molecular biology specifications and diagnostic requirements. Our buffer production ensures consistent pH performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Molecular Biology Grade Specifications
pH 8.3 ± 0.1 Verified
Buffer Performance Testing
Ionic Strength Analysis
Nuclease Testing
Certificate of Analysis
Temperature-Controlled Storage

Advanced Chemical Properties & Performance

Biotechnology-grade TAE/TBE Buffers exhibit exceptional properties essential for nucleic acid electrophoresis and gel analysis. The ultra-pure composition ensures optimal DNA/RNA migration patterns and consistent gel performance in demanding molecular biology procedures.

Buffer Composition
TAE Buffer (1X): 40 mM Tris-acetate, 1 mM EDTA pH 8.0
TBE Buffer (1X): 89 mM Tris-borate, 2 mM EDTA pH 8.3
Ionic Strength (TAE): Low ionic strength
Ionic Strength (TBE): Higher ionic strength
Physical Properties
Conductivity (TAE 1X): 1.2-1.5 mS/cm at 25°C
Conductivity (TBE 1X): 8.0-9.0 mS/cm at 25°C
pH Stability: Excellent buffering capacity
Temperature Coefficient: Minimal pH drift with temperature
Electrophoresis Performance
DNA Resolution (TAE): Optimal for large DNA fragments >1 kb
DNA Resolution (TBE): Superior for small DNA fragments <1 kb
Migration Speed: TAE: slower, TBE: faster
Heat Generation: TAE: lower, TBE: higher
Purity Specifications
Heavy Metals: ≤ 5 ppm
DNase/RNase: None detected
Endotoxin: ≤ 1 EU/mL
pH Accuracy: ±0.1 pH units
Stability Properties
Shelf Life (Powder): 3 years at room temperature
Solution Stability: 6 months at 4°C
Autoclave Stability: Stable to standard autoclave cycles
Freeze-Thaw Cycles: Stable to multiple cycles

Performance Characteristics

Detailed performance metrics demonstrate Biotechnology Grade TAE/TBE Buffers superiority in nucleic acid electrophoresis with exceptional resolution, reproducibility, and consistency across diverse gel electrophoresis applications.

Resolution Power

Band separation: Sharp, well-defined bands

Excellent DNA/RNA resolution
Buffer Capacity

pH stability: ±0.05 units during run

Robust buffering performance
Current Distribution

Voltage stability: Even current flow

Uniform migration patterns
Heat Management

TAE: minimal heating, TBE: controlled heating

Temperature-optimized performance
Reproducibility

Inter-gel variation: ±2% migration distance

Consistent analytical results
Run Time

TAE: 1-3 hours, TBE: 30-90 minutes

Optimized separation time

Safety Information

Generally safe for laboratory use with standard precautions. EDTA may cause mild eye irritation. Boric acid in TBE buffer may cause reproductive toxicity in high concentrations. Handle with standard laboratory protective equipment and ensure good ventilation.

Low Toxicity
Eye Protection
Good Ventilation

Storage & Handling

Store powder components at room temperature in dry conditions. Store prepared solutions at 4°C. Containers should be tightly sealed to prevent evaporation and contamination. Handle with standard laboratory protective equipment and use sterile technique when required.

Powder: room temperature
Solutions: store at 4°C
Keep containers sealed
Sterile technique when needed

Chemical Mechanisms & Reaction Pathways

TAE/TBE buffers exhibit electrophoretic properties through ionic strength control and pH buffering, enabling precise biotechnology applications with predictable reaction pathways and quantitative stoichiometry.

pH Buffering

Tris-acetate/borate maintains stable pH during electrophoresis

Quantitative pH control for biotechnology
Ionic Strength

Controlled conductivity for optimal nucleic acid migration

Essential for electrophoretic separation procedures
EDTA Chelation

Metal ion sequestration prevents nuclease activity

Enables controlled nucleic acid protection
Electrophoretic Migration

Participates in size-based nucleic acid separation

Controlled environment for biotechnology

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global biotechnology laboratory access with complete documentation packages supporting international standards and biotechnology method validations.

Electrophoresis Grade

Ultra-pure specifications for electrophoresis applications

ISO Standards

International standard for buffer specifications and testing

Molecular Biology Grade

High-purity specifications for nucleic acid work

REACH Registration

European Union chemical regulation compliance

Method Validation

Supported biotechnology method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's biotechnology team provides comprehensive method support, troubleshooting assistance, and biotechnology services to optimize TAE/TBE Buffers performance in your specific biotechnology applications.

Method Development
  • Biotechnology method optimization support
  • Buffer preparation protocol guidance
  • Electrophoresis optimization validation
  • Custom biotechnology procedures
Biotechnology Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • pH and conductivity testing
  • Method validation support
Technical Support
  • Biotechnology troubleshooting consultation
  • Buffer preparation training
  • Electrophoresis optimization guidance
  • Laboratory best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our Biotechnology Grade TAE/TBE Buffers production emphasizes environmental responsibility through sustainable manufacturing practices, waste minimization, and comprehensive environmental impact management for biotechnology laboratory operations.

Waste Minimization

Optimized formulation with minimal buffer waste generation

Water Treatment

Advanced wastewater treatment and neutralization systems

Clean Production

Energy-efficient preparation with emission controls

Safe Disposal

Comprehensive guidance for buffer waste management

ISO 14001

Environmental management system certified production

Container Recycling

Specialized buffer container recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art biotechnology reagent manufacturing facility employs advanced preparation technology and continuous monitoring systems to ensure consistent Biotechnology Grade TAE/TBE Buffers quality and performance across all production batches.

Production Process

Advanced buffer preparation in controlled environment

Multi-stage filtration for biotechnology grade quality
Quality Testing

22-point biotechnology testing protocol including pH and conductivity analysis

Electrophoretic verification and ion chromatography
Quality Systems

ISO 9001:2015 quality management with biotechnology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Sterile containers with controlled atmosphere packaging

Stability maintenance and contamination prevention

Market Applications & Performance Data

Comprehensive biotechnology laboratory data demonstrating Biotechnology Grade TAE/TBE Buffers effectiveness across diverse applications with quantified performance metrics and biotechnology method validations.

Biotechnology Laboratories
Precision: ±0.02 pH units in buffer systems Resolution: 95% nucleic acid band clarity Method Validation: 100% biotechnology compliance
Research Institutions
Publication Support: 2000+ cited methods Reproducibility: >99% inter-lab agreement Innovation: Custom buffer system development
Quality Control Labs
Reliability: 99.9% batch consistency Audit Success: 100% regulatory compliance Efficiency: 30% improved separation quality

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds biotechnology specifications with exceptional pH stability and conductivity control

Reliable Supply

Guaranteed availability with strategic inventory management and biotechnology-grade production scheduling

Biotechnology Expertise

Dedicated biotechnology team provides method development and troubleshooting support

Quality Assurance

Traceable certificates with comprehensive biotechnology data and method validation support

Global Standards

International compliance with electrophoresis and molecular biology specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with biotechnology laboratories and custom buffer development services