Safranin Stain
Biotechnology Counterstain
Bacterial Stains

Safranin (Stain)

High-quality Safranin essential for microbiology applications. Serves as the counterstain in Gram staining procedures for bacterial identification and classification, providing clear differential staining results for gram-negative bacteria.

  • High-Quality Counterstain
  • Gram Staining Protocol
  • Bacterial Identification
  • Clear Differential Results
  • Microbiology Grade
  • Research Validated
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Technical Specifications

Chemical Name: Safranin O
Alternative Names: Basic Red 2, Safranine
Molecular Formula: C₂₀H₁₉ClN₄
Molecular Weight: 350.84 g/mol
CAS Number: 477-73-6
Dye Content: ≥85% (certified)
Physical Form: Dark red crystalline powder
Solubility: Soluble in water and ethanol
Working Concentration: 0.25% aqueous solution
pH (1% solution): 4.0-6.0
Storage Temperature: Room temperature (dark place)
Packaging Options: 5g, 25g, 100g, 500g

Applications

Gram Staining
Bacterial Identification
Counterstaining
Endospore Staining
Microbiology
Educational Research
Clinical Diagnostics
Plant Histology
Cytology
Pathology
Food Microbiology
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized safranin grades tailored for specific staining and diagnostic applications, ensuring optimal color intensity and regulatory compliance across diverse microbiology requirements.

Laboratory Grade
Dye content: ≥85% Color intensity: High Heavy metals: <20 ppm Application: General staining
Certified Grade
Dye content: ≥90% Batch tested: Yes Certificate: Included Application: Clinical use
Ultra-Pure Grade
Dye content: ≥95% Heavy metals: <5 ppm Optical clarity: Excellent Application: Research
Diagnostic Grade
Dye content: ≥85% (USP) FDA: Compliant Validation: Clinical Application: Diagnostics

Quality Standards

DRAVYOM's Safranin is manufactured under stringent quality control protocols, meeting international dye standards including certified and diagnostic grade specifications. Our dye production ensures consistent color performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Certified Grade Specifications
≥85% Dye Content Verified
Staining Performance Testing
Color Intensity Analysis
pH Testing
Certificate of Analysis
Light-Protected Storage

Advanced Chemical Properties & Performance

Biotechnology-grade Safranin exhibits exceptional properties essential for microscopy staining and cell viability assays. Its ultra-pure composition and consistent dye performance ensure reliable results in demanding biological research applications.

Chemical Properties
Molecular Weight: 350.84 g/mol
Chemical Formula: C₂₀H₁₉ClN₄
IUPAC Name: 3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chloride
Dye Class: Basic phenazinium dye
Physical Properties
Appearance: Red-brown crystalline powder
Solubility (water): 5-10 mg/mL
Solubility (ethanol): 2-5 mg/mL
Absorption Maximum: 530 nm
Staining Properties
Counterstain Use: Gram staining, endospore stains
Cell Wall Affinity: High affinity for gram-positive bacteria
Nuclear Staining: Excellent chromatin visualization
Viability Assays: Live cell detection in plant tissues
Purity Specifications
Dye Content: ≥ 85% (spectrophotometric)
Water Content: ≤ 5%
Heavy Metals: ≤ 50 ppm
Chloride Content: 8-12% (theoretical 10.1%)
Stability Properties
Shelf Life: 3 years at room temperature
Light Sensitivity: Protect from direct sunlight
Solution Stability: 6 months at 4°C in dark
pH Stability: Stable pH 4-9

Performance Characteristics

Detailed performance metrics demonstrate Biotechnology Grade Safranin superiority in microscopy applications with exceptional staining consistency, color intensity, and reproducibility across diverse biological specimens.

Staining Intensity

Color development: Consistent red-pink coloration

Reliable contrast enhancement
Cellular Penetration

Cell wall penetration: Rapid and uniform

Excellent tissue permeability
Working Concentration

Optimal concentration: 0.25-0.5% aqueous

Economic dye usage
Temperature Tolerance

Working range: 15-40°C

Room temperature compatibility
Batch Consistency

Color variation: ±5% between lots

Reproducible staining results
Staining Time

Optimal staining: 30-60 seconds

Rapid protocol compatibility

Safety Information

May cause skin and eye irritation. Avoid inhalation of dust. Handle with standard laboratory protective equipment including gloves, safety goggles, and work in well-ventilated areas. Store in cool, dry conditions protected from light.

Mild Irritant
Light Sensitive
Eye Protection

Storage & Handling

Store at room temperature in original amber containers protected from light and moisture. Keep containers tightly closed when not in use. Handle with standard laboratory protective equipment and avoid generating dust during weighing.

Store at room temperature
Protect from light
Keep containers sealed
Use in ventilated area

Chemical Mechanisms & Reaction Pathways

Safranin exhibits cationic dye properties through phenazine structure and positive charge distribution, enabling precise biotechnology applications with predictable reaction pathways and quantitative stoichiometry.

DNA Binding

Electrostatic binding to negatively charged DNA backbone

Quantitative nucleic acid staining for biotechnology
Fluorescence Properties

Red fluorescence emission upon nucleic acid binding

Essential for fluorescence microscopy procedures
Phenazine Structure

Tricyclic aromatic system with conjugated electron system

Enables controlled fluorescence detection
Counterstaining

Participates in contrast enhancement for cellular structures

Controlled environment for biotechnology

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global biotechnology laboratory access with complete documentation packages supporting international standards and biotechnology method validations.

Research Grade

High-purity specifications for biotechnology applications

ISO Standards

International standard for fluorescent dye specifications and testing

Histology Grade

Validated specifications for histological staining applications

REACH Registration

European Union chemical regulation compliance

Method Validation

Supported biotechnology method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's biotechnology team provides comprehensive method support, troubleshooting assistance, and biotechnology services to optimize Safranin performance in your specific biotechnology applications.

Method Development
  • Biotechnology method optimization support
  • Staining protocol guidance
  • Fluorescence optimization validation
  • Custom biotechnology procedures
Biotechnology Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Fluorescence activity testing
  • Method validation support
Technical Support
  • Biotechnology troubleshooting consultation
  • Storage and handling guidance
  • Staining optimization training
  • Laboratory best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our Biotechnology Grade Safranin production emphasizes environmental responsibility through sustainable manufacturing practices, waste minimization, and comprehensive environmental impact management for biotechnology laboratory operations.

Waste Minimization

Optimized synthesis with minimal dye waste generation

Water Treatment

Advanced wastewater treatment and color removal systems

Clean Production

Energy-efficient synthesis with emission controls

Safe Disposal

Comprehensive guidance for fluorescent dye waste management

ISO 14001

Environmental management system certified production

Container Recycling

Specialized dye container recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art biotechnology reagent manufacturing facility employs advanced synthesis technology and continuous monitoring systems to ensure consistent Biotechnology Grade Safranin quality and performance across all production batches.

Production Process

Advanced organic synthesis in controlled environment

Multi-stage purification for biotechnology grade quality
Quality Testing

20-point biotechnology testing protocol including purity and fluorescence analysis

Spectrofluorimetric verification and HPLC analysis
Quality Systems

ISO 9001:2015 quality management with biotechnology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Light-protected containers with desiccant packaging

Stability maintenance and contamination prevention

Market Applications & Performance Data

Comprehensive biotechnology laboratory data demonstrating Biotechnology Grade Safranin effectiveness across diverse applications with quantified performance metrics and biotechnology method validations.

Biotechnology Laboratories
Precision: ±0.1% RSD in fluorescence staining Staining Efficiency: 98% nucleic acid detection Method Validation: 100% biotechnology compliance
Research Institutions
Publication Support: 400+ cited methods Reproducibility: >99% inter-lab agreement Innovation: Custom staining solution development
Quality Control Labs
Reliability: 99.9% batch consistency Audit Success: 100% regulatory compliance Efficiency: 35% improved staining contrast

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds biotechnology specifications with exceptional fluorescence activity and photostability

Reliable Supply

Guaranteed availability with strategic inventory management and biotechnology-grade production scheduling

Biotechnology Expertise

Dedicated biotechnology team provides method development and troubleshooting support

Quality Assurance

Traceable certificates with comprehensive biotechnology data and method validation support

Global Standards

International compliance with research grade and histology specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with biotechnology laboratories and custom staining solution development services