Reverse Transcriptase
Biotechnology RT Enzyme
Molecular Biology Enzymes

Reverse Transcriptase

High-activity reverse transcriptase essential for molecular biology applications. Enables efficient cDNA synthesis from RNA templates for RT-PCR, gene expression analysis, and RNA research requiring DNA conversion.

  • High-Activity Enzyme
  • cDNA Synthesis
  • RT-PCR Applications
  • RNA Analysis
  • Gene Expression Studies
  • Research Validated
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Technical Specifications

Enzyme Name: M-MLV Reverse Transcriptase
Source: Moloney Murine Leukemia Virus
Unit Definition: 1 unit incorporates 1 nmol dNTP in 10 min
Concentration: 200 units/μL
RNase H Activity: Reduced (RNase H-)
Optimal Temperature: 42°C
Storage Buffer: 20 mM Tris-HCl, 50% glycerol
Template Length: Up to 12 kb
Half-life: 40 min at 42°C
3' to 5' exonuclease: None
Storage Temperature: -20°C
Packaging Options: 1000U, 5000U, 25000U

Applications

cDNA Synthesis
RT-PCR
RT-qPCR
RNA Sequencing
Gene Expression
Educational Research
Molecular Biology
RNA Analysis
Plant RNA Studies
Functional Genomics
Diagnostics
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized reverse transcriptase grades tailored for specific RNA-to-cDNA synthesis applications, ensuring optimal activity and regulatory compliance across diverse molecular biology requirements.

Research Grade
Activity: 200 U/μL Template: Up to 12 kb RNase H: Reduced Application: General RT-PCR
High Performance Grade
Activity: 500 U/μL Temperature: Up to 50°C Processivity: Enhanced Application: Long transcripts
Ultra-Pure Grade
Activity: 200 U/μL DNase: None detected Endotoxin: <0.1 EU/mL Application: Sensitive assays
Diagnostic Grade
Activity: 200 U/μL Validation: Clinical GMP: Compliant Application: Diagnostics

Quality Standards

DRAVYOM's Reverse Transcriptase is manufactured under stringent quality control protocols, meeting international enzyme standards including molecular biology specifications and diagnostic requirements. Our enzyme production ensures consistent activity and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Molecular Biology Grade Specifications
200 U/μL Activity Verified
Activity Testing
RNase H Testing
Thermal Stability Testing
Certificate of Analysis
Cold Chain Storage

Advanced Chemical Properties & Performance

Biotechnology-grade Reverse Transcriptase exhibits exceptional properties essential for cDNA synthesis and molecular cloning applications. Its ultra-pure composition and high specific activity ensure reliable performance in demanding molecular biology procedures.

Enzyme Properties
Molecular Weight: 66,000 Da
Enzyme Source: M-MLV derived
Template Specificity: RNA, DNA-RNA hybrids
RNase H Activity: Reduced (H⁻ mutant)
Activity Parameters
Specific Activity: ≥ 15,000 U/mg
Optimal Temperature: 42°C
Temperature Range: 37-50°C
Thermostability: 50°C for 60 minutes
Reaction Requirements
Buffer System: Tris-HCl pH 8.3
Mg²⁺ Requirement: 5 mM MgCl₂
dNTP Concentration: 0.5 mM each
Primer Requirement: 3'-OH primer essential
Quality Specifications
Purity: ≥ 99% (SDS-PAGE)
DNase Activity: None detected
RNase Activity: Minimal
Processivity: High (>8 kb transcripts)
Stability Properties
Storage Temperature: -20°C
Shelf Life: 2 years at -20°C
Working Stability: 1 week at 4°C
Freeze-Thaw Tolerance: 5 cycles maximum

Performance Characteristics

Detailed performance metrics demonstrate Biotechnology Grade Reverse Transcriptase superiority in cDNA synthesis with exceptional efficiency, precision, and reproducibility across diverse molecular biology applications.

Synthesis Efficiency

Full-length cDNA: >90% yield

High conversion efficiency
Processivity

Long transcript synthesis: >8 kb

Excellent read-through capability
Template Range

RNA size: 0.1-15 kb effective

Broad template compatibility
Thermostability

Active at 50°C for 60 minutes

Enhanced high-temperature performance
Fidelity

Error rate: 1 in 17,000 nucleotides

High-fidelity synthesis
Reaction Speed

Synthesis time: 30-60 minutes optimal

Rapid cDNA generation

Safety Information

Generally safe for laboratory use with standard precautions. Contains glycerol and buffer salts that may cause mild eye irritation. Handle with standard laboratory protective equipment including gloves and safety goggles. Store at -20°C and avoid repeated freeze-thaw cycles.

Low Toxicity
Keep Frozen
RNase-Free Environment

Storage & Handling

Store at -20°C in original packaging with glycerol cryoprotectant. Aliquot upon first use to minimize freeze-thaw cycles. Handle with RNase-free technique and work on ice during setup. Use dedicated pipettes and tips for RNA work.

Store at -20°C
Aliquot to minimize freeze-thaw
RNase-free technique required
Work on ice during use

Chemical Mechanisms & Reaction Pathways

Reverse transcriptase exhibits RNA-dependent DNA polymerase activity through template-directed synthesis and primer extension, enabling precise biotechnology applications with predictable reaction pathways and quantitative stoichiometry.

RNA Template Recognition

Specific binding and processing of RNA templates

Quantitative cDNA synthesis for biotechnology
Phosphodiester Formation

Metal-dependent nucleotidyl transfer with primer extension

Essential for reverse transcription procedures
Polymerase Activity

5' to 3' DNA synthesis from RNA template

Enables controlled cDNA synthesis
Mg²⁺ Cofactor Dependence

Participates in magnesium-dependent catalytic mechanism

Controlled environment for biotechnology

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global biotechnology laboratory access with complete documentation packages supporting international standards and biotechnology method validations.

Molecular Biology Grade

Ultra-pure specifications for molecular biology applications

ISO Standards

International standard for reverse transcriptase specifications and testing

Research Grade

High-activity specifications for research applications

REACH Registration

European Union chemical regulation compliance

Method Validation

Supported biotechnology method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's biotechnology team provides comprehensive method support, troubleshooting assistance, and biotechnology services to optimize Reverse Transcriptase performance in your specific biotechnology applications.

Method Development
  • Biotechnology method optimization support
  • cDNA synthesis protocol guidance
  • RT-PCR optimization validation
  • Custom biotechnology procedures
Biotechnology Services
  • Certificate of Analysis verification
  • Custom activity analysis
  • Thermostability testing
  • Method validation support
Technical Support
  • Biotechnology troubleshooting consultation
  • Storage and handling guidance
  • Enzyme optimization training
  • Laboratory best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our Biotechnology Grade Reverse Transcriptase production emphasizes environmental responsibility through sustainable manufacturing practices, waste minimization, and comprehensive environmental impact management for biotechnology laboratory operations.

Waste Minimization

Optimized fermentation with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Clean Production

Energy-efficient protein expression with emission controls

Safe Disposal

Comprehensive guidance for enzyme waste management

ISO 14001

Environmental management system certified production

Container Recycling

Specialized enzyme container recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art biotechnology reagent manufacturing facility employs advanced recombinant expression technology and continuous monitoring systems to ensure consistent Biotechnology Grade Reverse Transcriptase quality and performance across all production batches.

Production Process

Advanced recombinant expression and purification in controlled sterile environment

Multi-stage purification for biotechnology grade quality
Quality Testing

35-point biotechnology testing protocol including activity and thermostability analysis

RT-PCR verification and functional assays
Quality Systems

ISO 9001:2015 quality management with biotechnology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Enzyme storage buffer with glycerol stabilization

Activity maintenance and contamination prevention

Market Applications & Performance Data

Comprehensive biotechnology laboratory data demonstrating Biotechnology Grade Reverse Transcriptase effectiveness across diverse applications with quantified performance metrics and biotechnology method validations.

Biotechnology Laboratories
Precision: ±0.1% RSD in cDNA synthesis Conversion Efficiency: >95% RNA to cDNA Method Validation: 100% biotechnology compliance
Research Institutions
Publication Support: 950+ cited methods Reproducibility: >99% inter-lab agreement Innovation: Custom enzyme development
Quality Control Labs
Reliability: 99.9% batch consistency Audit Success: 100% regulatory compliance Efficiency: 60% improved RT-PCR success rate

DRAVYOM Competitive Advantages

Superior Activity

Consistently exceeds biotechnology specifications with exceptional enzymatic activity and thermostability

Reliable Supply

Guaranteed availability with strategic inventory management and biotechnology-grade production scheduling

Biotechnology Expertise

Dedicated biotechnology team provides method development and troubleshooting support

Quality Assurance

Traceable certificates with comprehensive biotechnology data and method validation support

Global Standards

International compliance with molecular biology and research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with biotechnology laboratories and custom enzyme development services