EcoRI Restriction Enzyme
Biotechnology Enzyme
Restriction Enzymes

Restriction Enzymes (EcoRI)

High-activity EcoRI restriction endonuclease for molecular biology applications. Provides precise DNA cutting at GAATTC recognition sequences for DNA cloning, analysis, and manipulation with consistent performance and reliability.

  • High-Activity Restriction Enzyme
  • Precise GAATTC Recognition
  • DNA Cloning Applications
  • Molecular Biology Grade
  • Consistent Performance
  • Research Validated
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Technical Specifications

Enzyme Name: EcoRI Restriction Endonuclease
Recognition Sequence: 5'-GAATTC-3' / 3'-CTTAAG-5'
EC Number: 3.1.21.4
Molecular Weight: 31 kDa (monomer)
Activity: 20,000 units/mL
Overhang Type: 5' sticky ends (4 nucleotides)
Buffer System: NEBuffer 3.1 (100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2, 100 μg/ml BSA, pH 7.9)
Incubation Temperature: 37°C
Heat Inactivation: 65°C for 20 minutes
Storage Buffer: 50% glycerol, 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, pH 7.4
Storage Temperature: -20°C
Packaging Options: 500 units, 2,500 units, 10,000 units

Applications

DNA Cloning
Restriction Mapping
Vector Preparation
Insert Preparation
Molecular Cloning
Educational Research
Genomic Analysis
Plasmid Construction
Plant Genomics
Gene Expression
Recombinant DNA
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized EcoRI grades tailored for specific molecular cloning and genomic applications, ensuring optimal cutting performance and regulatory compliance across diverse research applications.

Research Grade
Activity: 20,000 units/mL Specificity: >99% Exonuclease: None detected Application: General cloning
High-Fidelity Grade
Activity: 50,000 units/mL Star Activity: Minimal Quality: Stringent tested Application: Precision cloning
Ultra-Pure Grade
Activity: 100,000 units/mL Contaminants: <0.001% Endotoxin: <0.1 EU/μL Application: Sensitive applications
Clinical Grade
Activity: 20,000 units/mL GMP: Compliant Sterility: Tested Application: Clinical research

Quality Standards

DRAVYOM's EcoRI is manufactured under stringent quality control protocols, meeting international molecular biology standards including ISO, GMP, and research specifications. Our enzyme production ensures consistent cutting activity and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Molecular Biology Grade
20,000 U/mL Activity Verified
Specificity Testing
Star Activity Assessment
Batch-to-Batch Consistency
Certificate of Analysis
Cold Chain Logistics

Advanced Chemical Properties & Performance

Biotechnology-grade EcoRI Restriction Enzyme exhibits exceptional properties essential for DNA cleavage and molecular cloning applications. Its ultra-pure composition and high specific activity ensure reliable performance in demanding molecular biology procedures.

Enzyme Properties
Molecular Weight: 31,000 Da
Recognition Sequence: 5'-GAATTC-3'
Cleavage Site: G↓AATTC
End Type: 5' sticky ends
Activity Parameters
Specific Activity: ≥ 20,000 U/mg
Optimal Temperature: 37°C
Temperature Range: 25-37°C
Heat Inactivation: 65°C for 20 minutes
Buffer Requirements
Optimal Buffer: EcoRI Buffer (1x)
Salt Concentration: 50 mM NaCl optimal
pH Range: 7.5-8.0
Mg²⁺ Requirement: 10 mM MgCl₂
Quality Specifications
Purity: ≥ 99% (SDS-PAGE)
Nuclease Activity: None detected
Ligase Activity: None detected
Phosphatase Activity: None detected
Stability Properties
Storage Temperature: -20°C
Shelf Life: 2 years at -20°C
Freeze-Thaw Stability: Stable to 10 cycles
Working Dilution Stability: 1 week at 4°C

Performance Characteristics

Detailed performance metrics demonstrate Biotechnology Grade EcoRI superiority in DNA digestion with exceptional specificity, precision, and reproducibility across diverse molecular cloning applications.

Cleavage Efficiency

Complete digestion: >95% in 1 hour

High catalytic efficiency
Sequence Specificity

Recognition: 100% sequence fidelity

No star activity detected
Unit Definition

1 unit: Digests 1 μg λ DNA in 1 hour

Standardized activity
Temperature Tolerance

Active range: 25-42°C

Robust temperature stability
Batch Consistency

Activity variation: ±10% between lots

Reliable performance
Reaction Kinetics

Optimal time: 1-3 hours at 37°C

Predictable reaction time

Safety Information

Generally safe for laboratory use with standard precautions. Contains glycerol and buffer salts that may cause mild eye irritation. Handle with standard laboratory protective equipment including gloves and safety goggles. Store at -20°C and avoid repeated freeze-thaw cycles.

Low Toxicity
Keep Frozen
Eye Protection Recommended

Storage & Handling

Store at -20°C in original packaging. Avoid repeated freeze-thaw cycles by aliquoting upon first use. Handle with standard laboratory protective equipment and work on ice when preparing reactions. Use clean, nuclease-free technique throughout.

Store at -20°C
Aliquot to avoid freeze-thaw
Work on ice during use
Nuclease-free technique

Chemical Mechanisms & Reaction Pathways

EcoRI exhibits sequence-specific DNA cleavage through homodimeric endonuclease activity and palindromic recognition, enabling precise biotechnology applications with predictable reaction pathways and quantitative stoichiometry.

DNA Recognition

Specific recognition of GAATTC palindromic sequence

Quantitative sequence-specific cleavage for biotechnology
Phosphodiester Hydrolysis

Metal-dependent hydrolysis creating 5'-phosphate sticky ends

Essential for cloning procedures
Homodimer Activity

Functional enzyme operates as homodimeric complex

Enables controlled DNA manipulation
Mg²⁺ Cofactor Dependence

Participates in magnesium-dependent catalytic mechanism

Controlled environment for biotechnology

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global biotechnology laboratory access with complete documentation packages supporting international standards and biotechnology method validations.

Molecular Biology Grade

Ultra-pure specifications for molecular biology applications

ISO Standards

International standard for restriction enzyme specifications and testing

Research Grade

High-activity specifications for research applications

REACH Registration

European Union chemical regulation compliance

Method Validation

Supported biotechnology method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's biotechnology team provides comprehensive method support, troubleshooting assistance, and biotechnology services to optimize EcoRI performance in your specific biotechnology applications.

Method Development
  • Biotechnology method optimization support
  • Restriction digestion protocol guidance
  • Cloning strategy validation
  • Custom biotechnology procedures
Biotechnology Services
  • Certificate of Analysis verification
  • Custom activity analysis
  • Specificity testing
  • Method validation support
Technical Support
  • Biotechnology troubleshooting consultation
  • Storage and handling guidance
  • Enzyme optimization training
  • Laboratory best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our Biotechnology Grade EcoRI production emphasizes environmental responsibility through sustainable manufacturing practices, waste minimization, and comprehensive environmental impact management for biotechnology laboratory operations.

Waste Minimization

Optimized fermentation with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Clean Production

Energy-efficient protein expression with emission controls

Safe Disposal

Comprehensive guidance for enzyme waste management

ISO 14001

Environmental management system certified production

Container Recycling

Specialized enzyme container recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art biotechnology reagent manufacturing facility employs advanced recombinant expression technology and continuous monitoring systems to ensure consistent Biotechnology Grade EcoRI quality and performance across all production batches.

Production Process

Advanced recombinant expression and purification in controlled sterile environment

Multi-stage purification for biotechnology grade quality
Quality Testing

30-point biotechnology testing protocol including activity and specificity analysis

Gel electrophoresis verification and functional assays
Quality Systems

ISO 9001:2015 quality management with biotechnology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Enzyme storage buffer with glycerol stabilization

Activity maintenance and contamination prevention

Market Applications & Performance Data

Comprehensive biotechnology laboratory data demonstrating Biotechnology Grade EcoRI effectiveness across diverse applications with quantified performance metrics and biotechnology method validations.

Biotechnology Laboratories
Precision: ±0.1% RSD in restriction digestion Cleavage Efficiency: >95% complete digestion Method Validation: 100% biotechnology compliance
Research Institutions
Publication Support: 850+ cited methods Reproducibility: >99% inter-lab agreement Innovation: Custom enzyme development
Quality Control Labs
Reliability: 99.9% batch consistency Audit Success: 100% regulatory compliance Efficiency: 50% improved cloning success rate

DRAVYOM Competitive Advantages

Superior Activity

Consistently exceeds biotechnology specifications with exceptional enzymatic activity and specificity

Reliable Supply

Guaranteed availability with strategic inventory management and biotechnology-grade production scheduling

Biotechnology Expertise

Dedicated biotechnology team provides method development and troubleshooting support

Quality Assurance

Traceable certificates with comprehensive biotechnology data and method validation support

Global Standards

International compliance with molecular biology and research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with biotechnology laboratories and custom enzyme development services