Propidium Iodide Microscopy Dye
Biotechnology Premium
Fluorescent Dye & Stain

Propidium Iodide Microscopy Dye

High-quality Propidium Iodide for fluorescence microscopy, flow cytometry, and nucleic acid detection. Premium grade fluorescent dye manufactured for cell viability assays and DNA staining applications.

  • Fluorescent DNA Intercalator
  • Cell Viability Indicator
  • Flow Cytometry Compatible
  • High Fluorescence Intensity
  • Microscopy Grade Purity
  • Research Grade Quality

Technical Specifications

Chemical Name: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
CAS Number: 25535-16-4
Molecular Formula: C27H34I2N4
Molecular Weight: 668.4 g/mol
Physical State: Dark red crystalline powder
Solubility: Water, PBS, DMSO
Excitation Maximum: 535 nm
Emission Maximum: 617 nm (DNA-bound)
Storage Temperature: 2-8°C (Protected from light)
Purity: ≥94% (HPLC)
Working Concentration: 1-10 μg/mL
Packaging Options: 10mg, 25mg, 100mg containers

Applications

Dead Cell Identification
Flow Cytometry
DNA/RNA Staining
Fluorescence Microscopy
Cell Viability Assays
Research Applications
Apoptosis Studies
Cell Cycle Analysis
Plant Cell Studies
Membrane Permeability
Live/Dead Discrimination
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized propidium iodide grades tailored for specific flow cytometry and fluorescence applications, ensuring optimal staining performance and regulatory compliance across diverse cell biology applications.

Research Grade
Purity: ≥94% (HPLC) Fluorescence: High intensity Background: Low Application: General research
Flow Cytometry Grade
Purity: ≥97% (HPLC) Aggregation: Minimal Consistency: Batch-validated Application: Flow cytometry
Microscopy Grade
Purity: ≥98% (HPLC) Photobleaching: Reduced Signal-to-Noise: High Application: Fluorescence microscopy
Clinical Grade
Purity: ≥99% (HPLC) Endotoxin: <0.25 EU/mg Sterility: Tested Application: Clinical research

Quality Standards

DRAVYOM's Propidium Iodide is manufactured under stringent quality control protocols, meeting international fluorescence standards including ISO, research, and clinical specifications. Our dye-grade production ensures consistent fluorescence performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Fluorescence Grade Specifications
≥94% HPLC Verified Purity
Fluorescence Intensity Testing
Spectral Characterization
Light Stability Assessment
Certificate of Analysis
Cold Chain Logistics

Advanced Chemical Properties & Performance

Biotechnology-grade Propidium Iodide exhibits exceptional properties essential for nucleic acid staining and fluorescence applications. Its ultra-pure composition ensures reliable performance in demanding biological procedures and research applications.

Chemical Properties
Molecular Weight: 668.4 g/mol
Chemical Formula: C₂₇H₃₄I₂N₄
Solubility: Soluble in water, DMSO
Structure Type: Intercalating dye
Physical Properties
Appearance: Dark red crystalline powder
Melting Point: >300°C (decomposes)
Hygroscopicity: Hygroscopic
Storage Stability: Store at -20°C
Optical Properties
Excitation Maximum: 535 nm
Emission Maximum: 617 nm
Quantum Yield: 0.18 (bound to DNA)
Fluorescence Enhancement: 30-fold upon DNA binding
Purity Specifications
Purity: ≥ 95% (HPLC)
Water Content: ≤ 5%
Heavy Metals: ≤ 10 ppm
Fluorescence Activity: Tested and verified
Stability Properties
Shelf Life: 2 years at -20°C
Light Sensitivity: Protect from light
Solution Stability: 1 month at 4°C
Freeze-Thaw Cycles: Stable to 5 cycles

Performance Characteristics

Detailed performance metrics demonstrate Biotechnology Grade Propidium Iodide superiority in nucleic acid staining with exceptional specificity, precision, and reproducibility across diverse fluorescence applications.

DNA Binding Affinity

Binding constant: 10⁶ M⁻¹ for dsDNA

Strong intercalation binding
Fluorescence Enhancement

Signal increase: 30-fold upon binding

Excellent signal-to-noise ratio
Cell Permeability

Live cell exclusion: Membrane impermeant

Dead cell specific staining
Temperature Stability

Working range: 4-37°C optimal

Stable under physiological conditions
Reproducibility

CV: <5% between assays

Excellent analytical precision
Working Solutions

Stability: 1 month at 4°C in dark

Ready-to-use convenience

Safety Information

Potential mutagen and carcinogen. Handle with extreme care using appropriate protective equipment including double gloves, safety goggles, and lab coat. Work in a well-ventilated area or chemical fume hood. Avoid skin contact and inhalation. Dispose according to hazardous waste regulations.

Potential Carcinogen
Potential Mutagen
Avoid Skin Contact

Storage & Handling

Store at -20°C in original sealed containers protected from light and moisture. Keep frozen until use and avoid repeated freeze-thaw cycles. Handle with appropriate personal protective equipment in a chemical fume hood. Prepare working solutions in subdued lighting.

Store at -20°C
Protect from light
Use fume hood for handling
Hazardous waste disposal

Chemical Mechanisms & Reaction Pathways

Propidium iodide exhibits specific DNA binding properties through intercalation and fluorescence emission, enabling precise biotechnology applications with predictable reaction pathways and quantitative stoichiometry.

DNA Intercalation

Double helix intercalation with base pair binding and unwinding

Quantitative DNA binding for biotechnology
Fluorescence Enhancement

50-fold fluorescence increase upon DNA binding

Essential for cell viability detection
Membrane Permeability

Selective entry through damaged cell membranes only

Enables controlled viability assessment
Spectroscopic Properties

Participates in excitation at 536nm and emission at 617nm

Controlled detection environment for biotechnology

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global biotechnology laboratory access with complete documentation packages supporting international standards and biotechnology method validations.

Research Grade

High-purity specifications for biotechnology applications

ISO Standards

International standard for fluorescent dye specifications and testing

Cell Biology Grade

Validated specifications for cell viability applications

REACH Registration

European Union chemical regulation compliance

Method Validation

Supported biotechnology method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's biotechnology team provides comprehensive method support, troubleshooting assistance, and biotechnology services to optimize Propidium Iodide performance in your specific biotechnology applications.

Method Development
  • Biotechnology method optimization support
  • Flow cytometry protocol guidance
  • Viability assay validation
  • Custom biotechnology procedures
Biotechnology Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Fluorescence activity testing
  • Method validation support
Technical Support
  • Biotechnology troubleshooting consultation
  • Safety protocols and training
  • Handling and storage guidance
  • Laboratory safety practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our Biotechnology Grade Propidium Iodide production emphasizes environmental responsibility through sustainable manufacturing practices, waste minimization, and comprehensive environmental impact management for biotechnology laboratory operations.

Waste Minimization

Optimized synthesis with minimal hazardous waste generation

Water Treatment

Advanced wastewater treatment and neutralization systems

Clean Production

Energy-efficient synthesis with emission controls

Safe Disposal

Comprehensive guidance for fluorescent dye waste management

ISO 14001

Environmental management system certified production

Container Recycling

Specialized hazardous material container recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art biotechnology reagent manufacturing facility employs advanced synthesis technology and continuous monitoring systems to ensure consistent Biotechnology Grade Propidium Iodide quality and performance across all production batches.

Production Process

Advanced organic synthesis in controlled light-protected environment

Multi-stage purification for biotechnology grade quality
Quality Testing

25-point biotechnology testing protocol including purity and fluorescence analysis

Spectrofluorimetric verification and HPLC analysis
Quality Systems

ISO 9001:2015 quality management with biotechnology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Light-protected containers with desiccant packaging

Photostability maintenance and contamination prevention

Market Applications & Performance Data

Comprehensive biotechnology laboratory data demonstrating Biotechnology Grade Propidium Iodide effectiveness across diverse applications with quantified performance metrics and biotechnology method validations.

Biotechnology Laboratories
Precision: ±0.1% RSD in viability assays Detection Sensitivity: 98% viability discrimination Method Validation: 100% biotechnology compliance
Research Institutions
Publication Support: 750+ cited methods Reproducibility: >99% inter-lab agreement Innovation: Custom staining protocol development
Quality Control Labs
Reliability: 99.9% batch consistency Audit Success: 100% regulatory compliance Efficiency: 40% improved viability detection accuracy

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds biotechnology specifications with exceptional fluorescence activity and photostability

Reliable Supply

Guaranteed availability with strategic inventory management and biotechnology-grade production scheduling

Biotechnology Expertise

Dedicated biotechnology team provides method development and troubleshooting support

Quality Assurance

Traceable certificates with comprehensive biotechnology data and method validation support

Global Standards

International compliance with research grade and cell biology specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with biotechnology laboratories and custom staining solution development services