PMSF Protease Inhibitor
Biotechnology Protease Inhibitor
Enzyme Inhibitors

PMSF (Protease Inhibitor)

High-purity PMSF (Phenylmethylsulfonyl fluoride) essential for biotechnology applications. Provides effective protease inhibition for protein protection, enzyme studies, and biochemical procedures requiring controlled proteolytic activity prevention.

  • High-Purity Protease Inhibitor
  • Protein Protection
  • Enzyme Inhibition
  • Toxic - Handle with Care
  • Research Grade
  • Safety Protocols Required
Request Quote Safety Information

Technical Specifications

Chemical Name: Phenylmethylsulfonyl fluoride
CAS Number: 329-98-6
Molecular Formula: C7H7FO2S
Molecular Weight: 174.19 g/mol
Physical State: White to off-white crystalline powder
Melting Point: 91-93°C
Solubility: Isopropanol, ethanol (Limited in water)
Working Concentration: 0.1-1.0 mM (100x stock in isopropanol)
Storage Temperature: -20°C (Keep dry)
Purity: ≥98% (GC)
Inhibition Type: Irreversible serine protease inhibitor
Packaging Options: 1g, 5g, 25g containers

Applications

Protease Inhibition
Protein Purification
Cell Lysis Protection
Protein Studies
Enzyme Assays
Research Applications
Biochemical Studies
Structural Biology
Plant Protein Studies
Western Blotting
Protein Chemistry
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized PMSF grades tailored for specific protein research and biochemical applications, ensuring optimal protease inhibition and regulatory compliance across diverse laboratory applications.

Research Grade
Purity: ≥98% (GC) Water Content: ≤0.5% Inhibition: Effective Application: General research
Biochemical Grade
Purity: ≥99% (GC) Heavy Metals: <10 ppm Proteases: Inhibited Application: Protein purification
Ultra-Pure Grade
Purity: ≥99.5% (GC) Trace Organics: <5 ppm Moisture: ≤0.1% Application: Sensitive assays
Pharmaceutical Grade
Purity: ≥99.9% (GC) Endotoxin: <0.25 EU/mg Sterility: Tested Application: Drug development

Quality Standards

DRAVYOM's PMSF is manufactured under stringent quality control protocols, meeting international biochemical standards including cGMP, ISO, and safety specifications. Our inhibitor-grade production ensures consistent protease inhibition and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
cGMP Grade Specifications
≥98% GC Verified Purity
Inhibition Activity Testing
Stability Assessment
Safety Compliance
Certificate of Analysis
Cold Chain Logistics

Advanced Chemical Properties & Performance

Biotechnology-grade PMSF (Phenylmethylsulfonyl fluoride) exhibits exceptional properties essential for protease inhibition and protein purification applications. Its ultra-pure composition and high specific activity ensure reliable performance in demanding biochemical procedures.

Chemical Properties
Molecular Formula: C₇H₇FO₂S
Molecular Weight: 174.19 g/mol
IUPAC Name: Phenylmethylsulfonyl fluoride
CAS Number: 329-98-6
Physical Properties
Appearance: White to off-white crystalline powder
Melting Point: 91-93°C
Solubility (water): Practically insoluble
Solubility (ethanol): Soluble (stock solution preparation)
Inhibition Properties
Target Enzymes: Serine proteases
Inhibition Type: Irreversible covalent modification
Working Concentration: 0.1-1.0 mM
Reaction Time: 10-30 minutes for complete inhibition
Purity Specifications
Purity: ≥ 99.0% (HPLC)
Water Content: ≤ 0.5%
Heavy Metals: ≤ 10 ppm
Residual Solvents: Within ICH guidelines
Stability Properties
Shelf Life (solid): 3 years at -20°C
Aqueous Stability: Rapidly hydrolyzes (t₁/₂ ~35 min)
Stock Solution: Stable in anhydrous solvents
Temperature Sensitivity: Store frozen for long-term stability

Performance Characteristics

Detailed performance metrics demonstrate Biotechnology Grade PMSF superiority in protease inhibition with exceptional selectivity, rapid action, and reproducibility across diverse protein purification applications.

Inhibition Efficiency

Protease inhibition: >99% at 1 mM

Complete serine protease blocking
Selectivity

Target specificity: Serine proteases only

No effect on other proteases
Reaction Speed

Inhibition kinetics: Complete in 10-30 min

Rapid enzyme inactivation
Temperature Stability

Active range: 4-37°C

Working temperature compatible
Batch Consistency

Activity variation: ±5% between lots

Reliable inhibition performance
Aqueous Lifetime

Half-life in water: ~35 minutes at pH 8

Use immediately after dilution

Safety Information

Highly toxic compound that can cause severe poisoning. Suspected carcinogen and cholinesterase inhibitor. Handle only with full protective equipment in fume hood. Avoid skin contact and inhalation. Keep antidotal treatment available. Requires specialized disposal procedures.

Highly Toxic
Suspected Carcinogen
Neurotoxic

Storage & Handling

Store at -20°C in tightly sealed containers under anhydrous conditions. Handle only in fume hood with full protective equipment including chemical-resistant gloves. Prepare fresh solutions immediately before use. Dispose according to hazardous waste regulations.

Store at -20°C
Fume hood required
Chemical-resistant gloves
Hazardous waste disposal

Chemical Mechanisms & Reaction Pathways

PMSF exhibits irreversible serine protease inhibition through active site modification and covalent bond formation, enabling precise biotechnology applications with predictable reaction pathways and quantitative stoichiometry.

Protease Inhibition

Irreversible sulfonyl fluoride reaction with serine active sites

Quantitative enzyme inhibition for biotechnology
Covalent Modification

Selective serine hydroxyl group targeting in active sites

Essential for protein stabilization procedures
Protein Protection

Prevention of proteolytic degradation during purification

Enables controlled protein isolation
Hydrolysis Susceptibility

Participates in aqueous hydrolysis with half-life considerations

Controlled inhibitor activity for biotechnology

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global biotechnology laboratory access with complete documentation packages supporting international standards and biotechnology method validations.

Research Grade

High-purity specifications for biotechnology applications

ISO Standards

International standard for protease inhibitor specifications and testing

Molecular Biology Grade

Ultra-pure specifications for molecular biology applications

REACH Registration

European Union chemical regulation compliance

Method Validation

Supported biotechnology method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's biotechnology team provides comprehensive method support, troubleshooting assistance, and biotechnology services to optimize PMSF performance in your specific biotechnology applications.

Method Development
  • Biotechnology method optimization support
  • Protease inhibition protocol guidance
  • Protein purification validation
  • Custom biotechnology procedures
Biotechnology Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Inhibition activity testing
  • Method validation support
Technical Support
  • Biotechnology troubleshooting consultation
  • Safety protocols and training
  • Handling and storage guidance
  • Laboratory safety practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our Biotechnology Grade PMSF production emphasizes environmental responsibility through sustainable manufacturing practices, waste minimization, and comprehensive environmental impact management for biotechnology laboratory operations.

Waste Minimization

Optimized synthesis with minimal hazardous waste generation

Water Treatment

Advanced wastewater treatment and neutralization systems

Clean Production

Energy-efficient synthesis with emission controls

Safe Disposal

Comprehensive guidance for hazardous chemical waste management

ISO 14001

Environmental management system certified production

Container Recycling

Specialized hazardous waste container recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art biotechnology reagent manufacturing facility employs advanced synthesis technology and continuous monitoring systems to ensure consistent Biotechnology Grade PMSF quality and performance across all production batches.

Production Process

Advanced organic synthesis in controlled atmosphere environment

Multi-stage purification for biotechnology grade quality
Quality Testing

20-point biotechnology testing protocol including purity and inhibition activity

GC-MS verification and enzymatic activity analysis
Quality Systems

ISO 9001:2015 quality management with biotechnology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Anhydrous containers with inert atmosphere packaging

Stability maintenance and contamination prevention

Market Applications & Performance Data

Comprehensive biotechnology laboratory data demonstrating Biotechnology Grade PMSF effectiveness across diverse applications with quantified performance metrics and biotechnology method validations.

Biotechnology Laboratories
Precision: ±0.1% RSD in inhibition assays Inhibition Efficiency: 99.5% protease inhibition Method Validation: 100% biotechnology compliance
Research Institutions
Publication Support: 650+ cited methods Reproducibility: >99% inter-lab agreement Innovation: Custom inhibitor development
Quality Control Labs
Reliability: 99.9% batch consistency Audit Success: 100% regulatory compliance Efficiency: 50% improved protein recovery

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds biotechnology specifications with ultra-high purity and exceptional inhibition activity

Reliable Supply

Guaranteed availability with strategic inventory management and biotechnology-grade production scheduling

Biotechnology Expertise

Dedicated biotechnology team provides method development and troubleshooting support

Quality Assurance

Traceable certificates with comprehensive biotechnology data and method validation support

Global Standards

International compliance with research grade and molecular biology specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with biotechnology laboratories and custom reagent development services