PBS Buffer (Phosphate-Buffered Saline)
Biotechnology Buffer
Cell Culture Buffers

PBS Buffer (Phosphate-Buffered Saline)

High-quality PBS buffer essential for biotechnology applications. Provides physiological pH buffering and isotonic conditions for cell culture, washing procedures, biological assays, and molecular biology techniques.

  • High-Quality Buffer System
  • Physiological pH Buffering
  • Isotonic Conditions
  • Cell Culture Compatible
  • Biological Assays
  • Research Validated

Technical Specifications

Chemical Name: Phosphate Buffered Saline
Composition: NaCl, KCl, Na₂HPO₄, KH₂PO₄
pH: 7.4 ± 0.1
Osmolarity: 290-310 mOsm/kg
Ionic Strength: 0.15 M
Physical Form: Clear, colorless solution or powder
Sterility: Sterile filtered (0.2 μm)
Buffer Capacity: pH 6.8-8.2
Endotoxin Level: <0.1 EU/mL
Stability: 2 years at 2-8°C
Storage Temperature: Room temperature or 2-8°C
Packaging Options: 500mL, 1L bottles; 1L, 10L powder

Applications

Cell Culture
Cell Washing
Immunofluorescence
Flow Cytometry
Western Blotting
Educational Research
Histology
Molecular Biology
Plant Cell Studies
ELISA
Protein Purification
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized PBS buffer grades tailored for specific cell culture and laboratory applications, ensuring optimal pH stability and regulatory compliance across diverse biotechnology requirements.

Research Grade
pH: 7.4 ± 0.1 Osmolarity: 290-310 mOsm Endotoxin: <1 EU/mL Application: General research
Cell Culture Grade
pH: 7.4 ± 0.05 Sterility: Assured Endotoxin: <0.1 EU/mL Application: Cell culture
Ultra-Pure Grade
pH: 7.4 ± 0.02 Heavy metals: <5 ppm Pyrogens: None detected Application: Sensitive assays
GMP Grade
pH: 7.4 ± 0.1 (USP) GMP: Compliant Validation: Complete Application: Pharmaceutical

Quality Standards

DRAVYOM's PBS Buffer is manufactured under stringent quality control protocols, meeting international buffer standards including cell culture specifications and GMP requirements. Our buffer production ensures consistent pH performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Cell Culture Grade Specifications
pH 7.4 ± 0.1 Verified
Osmolarity Testing
Endotoxin Testing
Sterility Testing
Certificate of Analysis
Cold Chain Storage

Advanced Chemical Properties & Performance

Biotechnology-grade PBS Buffer exhibits exceptional properties essential for cell culture, molecular biology, and protein biochemistry applications. Its ultra-pure composition and precise formulation ensure reliable performance in demanding biological procedures and research applications.

Buffer Properties
pH (25°C): 7.4 ± 0.1
Osmolality: 280-300 mOsm/kg
Ionic Strength: 0.163 M
Buffering Capacity: Excellent at physiological pH
Composition
NaCl: 137 mM
KCl: 2.7 mM
Na₂HPO₄: 10 mM
KH₂PO₄: 1.8 mM
Biological Performance
Cell Viability Maintenance: 99% for standard cell lines
Protein Stability: Maintains native conformation
Enzyme Activity: Preserves physiological activity
Washing Efficiency: Complete removal of media components
Purity Specifications
Endotoxin Level: ≤ 0.25 EU/mL
Sterility: Sterile filtered (0.1 μm)
Heavy Metals: ≤ 5 ppm
Particulates: ≤ 10 particles/mL (≥10 μm)
Stability Properties
Shelf Life: 2 years at 2-8°C
pH Stability: ±0.1 pH units for 18 months
Freeze-Thaw Cycles: Up to 5 cycles at -20°C
Room Temperature: Stable for 48 hours

Performance Characteristics

Detailed performance metrics demonstrate Biotechnology Grade PBS Buffer superiority in cell culture applications with exceptional pH stability, biocompatibility, and reproducibility across diverse biological procedures and research protocols.

pH Stability

pH variation: ±0.05 units over 24 hours

Exceptional buffering consistency
Cell Compatibility

Cell viability: 99% maintenance rate

Superior biocompatibility
Washing Efficiency

Contaminant removal: 99.9% effective

Complete cleaning performance
Temperature Stability

Working range: 4-37°C stable

Versatile storage conditions
Batch Consistency

Variation: ±1% osmolality between batches

Reliable performance reproducibility
Shelf Stability

2 years refrigerated, 48 hours room temp

Extended biotechnology grade integrity

Safety Information

Generally considered safe for laboratory use with standard precautions. Non-toxic aqueous solution suitable for cell culture applications. Handle with standard laboratory protective equipment including gloves and safety goggles. Ensure sterile technique when working with cell cultures.

Non-Toxic
Sterile Solution
Cell Culture Grade

Storage & Handling

Store in original sealed containers at 2-8°C in a clean, dry environment. Keep containers tightly closed and maintain sterile conditions during use. Use aseptic technique when transferring and avoid contamination from non-sterile surfaces.

Refrigerated storage (2-8°C)
Maintain sterile conditions
Sealed sterile containers
Aseptic handling required

Chemical Mechanisms & Reaction Pathways

PBS buffer exhibits precise pH control through phosphate buffering system and ionic strength maintenance, enabling precise biotechnology applications with predictable reaction pathways and quantitative stoichiometry.

Phosphate Buffering

pH control: H₂PO₄⁻ ⇌ HPO₄²⁻ + H⁺ (pKa = 7.2)

Quantitative pH maintenance for biotechnology
Ionic Strength

Physiological ionic strength through sodium and potassium salts

Essential for cell culture procedures
Osmotic Balance

Physiological osmolarity maintenance for cell viability

Enables stable cellular environments
Protein Stability

Participates in protein conformation and enzyme activity maintenance

Controlled environment for biotechnology

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global biotechnology laboratory access with complete documentation packages supporting international standards and biotechnology method validations.

USP Grade Available

United States Pharmacopeia specifications for biotechnology analysis

ISO Standards

International standard for buffer specifications and testing

Cell Culture Grade

Sterile specifications for cell culture applications

REACH Registration

European Union chemical regulation compliance

Method Validation

Supported biotechnology method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's biotechnology team provides comprehensive method support, troubleshooting assistance, and biotechnology services to optimize PBS Buffer performance in your specific biotechnology applications.

Method Development
  • Biotechnology method optimization support
  • Buffer system guidance
  • Cell culture protocol validation
  • Custom biotechnology procedures
Biotechnology Services
  • Certificate of Analysis verification
  • Custom pH and osmolarity testing
  • Sterility validation
  • Method validation support
Technical Support
  • Biotechnology troubleshooting consultation
  • Storage and handling guidance
  • Sterile technique training
  • Laboratory best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our Biotechnology Grade PBS Buffer production emphasizes environmental responsibility through sustainable manufacturing practices, waste minimization, and comprehensive environmental impact management for biotechnology laboratory operations.

Waste Minimization

Optimized production with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Clean Production

Energy-efficient preparation with emission controls

Safe Disposal

Comprehensive guidance for laboratory waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sterile container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art biotechnology reagent manufacturing facility employs advanced sterile preparation technology and continuous monitoring systems to ensure consistent Biotechnology Grade PBS Buffer quality and performance across all production batches.

Production Process

Advanced sterile preparation and filtration in controlled clean-room environment

Multi-stage sterile processing for biotechnology grade quality
Quality Testing

15-point biotechnology testing protocol including pH, osmolarity, and sterility

Ion chromatography verification and microbiological testing
Quality Systems

ISO 9001:2015 quality management with biotechnology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Sterile containers with gamma irradiation sterilization

Sterility maintenance and contamination prevention

Market Applications & Performance Data

Comprehensive biotechnology laboratory data demonstrating Biotechnology Grade PBS Buffer effectiveness across diverse applications with quantified performance metrics and biotechnology method validations.

Biotechnology Laboratories
Precision: ±0.01 pH units stability Cell Viability: 98% maintenance in culture Method Validation: 100% biotechnology compliance
Research Institutions
Publication Support: 800+ cited methods Reproducibility: >99% inter-lab agreement Innovation: Custom buffer development
Quality Control Labs
Reliability: 99.9% batch consistency Audit Success: 100% regulatory compliance Efficiency: 25% improved cell culture success

DRAVYOM Competitive Advantages

Superior Quality

Consistently exceeds biotechnology specifications with precise pH control and exceptional buffering performance

Reliable Supply

Guaranteed availability with strategic inventory management and biotechnology-grade production scheduling

Biotechnology Expertise

Dedicated biotechnology team provides method development and troubleshooting support

Quality Assurance

Traceable certificates with comprehensive biotechnology data and method validation support

Global Standards

International compliance with USP, ISO, and cell culture specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with biotechnology laboratories and custom buffer development services