Methylene Blue
Biotechnology Vital Stain
Biological Stains

Methylene Blue

High-quality methylene blue essential for biotechnology applications. Provides reliable staining properties for cell viability testing, microscopy procedures, and biological staining applications requiring clear cellular visualization.

  • High-Quality Biological Stain
  • Cell Viability Testing
  • Microscopy Applications
  • Vital Staining
  • Clear Visualization
  • Research Validated
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Technical Specifications

Chemical Name: 3,7-bis(Dimethylamino)phenazathionium chloride
CAS Number: 61-73-4
Molecular Formula: C16H18ClN3S
Molecular Weight: 319.85 g/mol
Physical State: Dark green crystalline powder
Solubility: Water (43.6 g/L at 25°C), ethanol
Absorption Maximum: 664 nm (in water)
pH Stability: 6.0-8.0 (Optimal 7.0)
Storage Temperature: Room temperature (Protected from light)
Purity: ≥82% (Dye content)
Working Concentration: 0.01-0.1% (w/v)
Packaging Options: 25g, 100g, 500g containers

Applications

Cell Viability Testing
Vital Staining
Bacterial Staining
Redox Indicator
Cell Counting
Educational Microscopy
Flow Cytometry
Histology Staining
Plant Cell Studies
DNA Visualization
Research Applications
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized methylene blue grades tailored for specific microscopy and analytical applications, ensuring optimal staining performance and regulatory compliance across diverse laboratory applications.

Microscopy Grade
Dye Content: ≥82% Clarity: Clear solution Background Staining: Minimal Application: General microscopy
Biological Stain Grade
Dye Content: ≥85% pH Range: 6.0-8.0 Heavy Metals: <20 ppm Application: Cell viability studies
Analytical Grade
Dye Content: ≥90% Moisture: ≤5% Insoluble Matter: ≤0.1% Application: Analytical procedures
Clinical Grade
Dye Content: ≥95% Endotoxin: <10 EU/g Sterility: Tested Application: Clinical diagnostics

Quality Standards

DRAVYOM's Methylene Blue is manufactured under stringent quality control protocols, meeting international laboratory standards including USP, BP, and research specifications. Our dye-grade production ensures consistent staining performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
USP/BP Grade Specifications
≥82% Dye Content Verified
Staining Performance Testing
Color Consistency
Batch-to-Batch Consistency
Certificate of Analysis
Light-Protected Storage

Advanced Chemical Properties & Performance

Biotechnology-grade Methylene Blue exhibits exceptional properties essential for vital staining and molecular biology applications. Its ultra-pure composition and precise molecular structure ensure reliable performance in demanding biological procedures and research applications.

Dye Properties
Molecular Weight: 319.85 g/mol (trihydrate)
Chemical Formula: C₁₆H₁₈ClN₃S·3H₂O
Absorption Maximum: 665 nm (aqueous solution)
Molar Extinction Coefficient: 95,000 M⁻¹cm⁻¹
Physical Properties
Melting Point: 100-110°C (with decomposition)
Solubility (Water): 43.6 g/L at 25°C
pH Indicator Range: pH 0.0 (colorless) to 1.8 (blue)
Light Sensitivity: Photosensitive (requires dark storage)
Staining Performance
Cell Viability Staining: Selective for dead/damaged cells
Working Concentration: 0.1-1.0% typical solutions
Staining Time: 1-5 minutes for most applications
Redox Potential: +0.011 V (vs. NHE)
Purity Specifications
Dye Content: ≥ 82% (calculated on dry basis)
Water Content: ≤ 15%
Heavy Metals: ≤ 50 ppm
Insoluble Matter: ≤ 0.1%
Stability Properties
Shelf Life: 3 years (dark storage)
Solution Stability: 6 months in dark at 4°C
pH Stability: Stable pH 2-10
Temperature Stability: Stable below 30°C

Performance Characteristics

Detailed performance metrics demonstrate Biotechnology Grade Methylene Blue superiority in vital staining applications with exceptional color intensity, precision, and reproducibility across diverse biological procedures and microscopy protocols.

Staining Efficiency

Cell viability detection: 95-98% accuracy

Excellent vital stain performance
Color Intensity

Optical density: Consistent high contrast

Superior microscopy visibility
Solubility Performance

Dissolution: Complete in 2 minutes

Rapid staining solution preparation
Temperature Stability

Working range: 4-30°C optimal

Stable under laboratory conditions
Batch Consistency

Color variation: ±3% between batches

Reliable staining reproducibility
Shelf Stability

3 years dark storage, 6 months solutions

Extended biotechnology grade integrity

Safety Information

May cause mild irritation to eyes, skin, and respiratory tract. Avoid inhalation of powder and skin contact. Handle with standard laboratory protective equipment including gloves and safety goggles. Ensure proper ventilation during use and avoid ingestion.

Mild Irritant
Avoid Inhalation
Avoid Skin Contact

Storage & Handling

Store in original sealed containers in a cool, dry, dark environment away from light sources. Keep containers tightly closed and protect from moisture and direct sunlight. Prepare solutions in amber or dark containers and store at 4°C.

Cool storage (15-25°C)
Dark storage required
Amber containers for solutions
Keep dry environment

Chemical Mechanisms & Reaction Pathways

Methylene blue exhibits specific biochemical properties through redox reactions and cellular staining mechanisms, enabling precise biotechnology applications with predictable reaction pathways and quantitative stoichiometry.

Redox Reactions

Reversible oxidation-reduction: MB⁺ ⇌ MBH (colorless leucomethylene blue)

Quantitative redox indicator for biotechnology
Cellular Staining

Nucleic acid and protein binding through electrostatic interactions

Essential for viability assessment procedures
Photodynamic Action

Light-activated generation of reactive oxygen species

Enables antimicrobial and research applications
Membrane Interaction

Participates in membrane permeability and viability studies

Controlled membrane assessment for biotechnology

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global biotechnology laboratory access with complete documentation packages supporting international standards and biotechnology method validations.

USP Grade Available

United States Pharmacopeia specifications for biotechnology analysis

ISO Standards

International standard for biotechnology reagent specifications

EP Grade Available

European Pharmacopoeia specifications for biotechnology applications

REACH Registration

European Union chemical regulation compliance

Method Validation

Supported biotechnology method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's biotechnology team provides comprehensive method support, troubleshooting assistance, and biotechnology services to optimize Methylene Blue performance in your specific biotechnology applications.

Method Development
  • Biotechnology method optimization support
  • Staining protocol guidance
  • Viability assay validation
  • Custom biotechnology procedures
Biotechnology Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Spectroscopic characterization
  • Method validation support
Technical Support
  • Biotechnology troubleshooting consultation
  • Storage and handling guidance
  • Safety training and protocols
  • Laboratory best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our Biotechnology Grade Methylene Blue production emphasizes environmental responsibility through sustainable manufacturing practices, waste minimization, and comprehensive environmental impact management for biotechnology laboratory operations.

Waste Minimization

Optimized production with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Clean Production

Energy-efficient synthesis with emission controls

Safe Disposal

Comprehensive guidance for laboratory waste management

ISO 14001

Environmental management system certified production

Container Recycling

Container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art biotechnology reagent manufacturing facility employs advanced purification technology and continuous monitoring systems to ensure consistent Biotechnology Grade Methylene Blue quality and performance across all production batches.

Production Process

Advanced crystallization and purification in controlled clean-room environment

Multi-stage purification for biotechnology grade quality
Quality Testing

22-point biotechnology testing protocol including spectroscopic and purity analysis

UV-Vis verification and HPLC analysis
Quality Systems

ISO 9001:2015 quality management with biotechnology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Light-protected containers with moisture control packaging

Photostability maintenance and contamination prevention

Market Applications & Performance Data

Comprehensive biotechnology laboratory data demonstrating Biotechnology Grade Methylene Blue effectiveness across diverse applications with quantified performance metrics and biotechnology method validations.

Biotechnology Laboratories
Precision: ±0.02% RSD in viability assays Staining Efficiency: 95% cell discrimination Method Validation: 100% biotechnology compliance
Research Institutions
Publication Support: 400+ cited methods Reproducibility: >99% inter-lab agreement Innovation: Custom grade development
Quality Control Labs
Reliability: 99.9% batch consistency Audit Success: 100% regulatory compliance Efficiency: 30% faster viability testing

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds biotechnology specifications with ultra-low impurities and exceptional staining performance

Reliable Supply

Guaranteed availability with strategic inventory management and biotechnology-grade production scheduling

Biotechnology Expertise

Dedicated biotechnology team provides method development and troubleshooting support

Quality Assurance

Traceable certificates with comprehensive biotechnology data and method validation support

Global Standards

International compliance with USP, EP, and ISO specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with biotechnology laboratories and custom grade development services