Hoechst 33342 DNA Stain
Biotechnology Premium
Live Cell Nuclear Stain

Hoechst 33342 DNA Stain

High-quality Hoechst 33342 for live cell nuclear staining and DNA visualization. Premium grade cell-permeable fluorescent dye manufactured for nuclear imaging and live cell applications.

  • Cell-Permeable DNA Stain
  • Live Cell Compatible
  • UV/Blue Fluorescence
  • Nuclear Morphology Analysis
  • Flow Cytometry Compatible
  • Research Grade Quality
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Technical Specifications

Chemical Name: Hoechst 33342 (bis-Benzimide)
Molecular Formula: C₂₇H₂₈N₆O
Molecular Weight: 452.55 g/mol
CAS Number: 23491-52-3
Purity: ≥98% (HPLC)
Physical Form: Yellow crystalline powder
Solubility: Water, DMSO, ethanol
Excitation/Emission: 350 nm / 461 nm
Working Concentration: 0.5-2 μg/mL
Cell Permeability: Cell permeable
Storage Temperature: -20°C (protect from light)
Packaging Options: 1mg, 5mg, 10mg, 25mg

Applications

Live Cell Imaging
Nuclear Staining
DNA Staining
Flow Cytometry
Cell Cycle Analysis
Educational Research
Fluorescence Microscopy
Cell Viability Studies
Apoptosis Detection
Chromatin Structure
Drug Screening
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized Hoechst 33342 grades tailored for specific live cell imaging and DNA staining applications, ensuring optimal fluorescence and regulatory compliance across diverse cell biology requirements.

Research Grade
Purity: ≥98% (HPLC) Fluorescence: High Background: Low Application: General research
Cell Culture Grade
Purity: ≥99% (HPLC) Sterility: Assured Endotoxin: <0.1 EU/mg Application: Live cell imaging
Ultra-Pure Grade
Purity: ≥99.5% (HPLC) Heavy metals: <10 ppm Photo-stability: Enhanced Application: Sensitive imaging
Flow Cytometry Grade
Purity: ≥98% (HPLC) Specificity: High Precision: Optimized Application: Flow cytometry

Quality Standards

DRAVYOM's Hoechst 33342 is manufactured under stringent quality control protocols, meeting international fluorescent dye standards including research specifications and live cell imaging requirements. Our nuclear dye production ensures consistent performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Research Grade Specifications
≥98% Purity Verified (HPLC)
Fluorescence Testing
Spectral Analysis
Photo-stability Testing
Certificate of Analysis
Light-Protected Storage

Advanced Chemical Properties & Performance

Hoechst 33342 exhibits exceptional nuclear staining properties essential for live cell DNA visualization applications. Its cell permeability and high DNA affinity ensure reliable performance in demanding fluorescence microscopy protocols, cell cycle analysis studies, and apoptosis detection procedures.

DNA Dye Properties
Molecular Weight: 623.99 g/mol
Chemical Formula: C₂₇H₂₈N₆O·3HCl·3H₂O
Dye Type: Bisbenzimide nucleic acid stain
Binding Mode: Minor groove of DNA
Physical Properties
Appearance: Yellow crystalline powder
Solubility: Water soluble (10 mg/mL)
Excitation λmax: 350 nm
Emission λmax: 461 nm
Cellular Performance
Cell Permeability: Live cell permeable
DNA Selectivity: AT-rich regions preferred
Incubation Time: 10-30 minutes
Working Concentration: 1-10 μg/mL
Purity Specifications
Dye Purity: ≥ 98% (HPLC)
Water Content: ≤ 8% (trihydrate)
Heavy Metals: ≤ 10 ppm
Related Dyes: ≤ 1% each
Stability Properties
Shelf Life: 3 years (-20°C, dark)
Solution Stability: Weeks at 4°C in dark
Light Sensitivity: Protect from light
pH Stability: Stable pH 6-8

Performance Characteristics

Detailed performance metrics demonstrate Hoechst 33342 superiority in live cell nuclear staining applications with exceptional cell permeability, DNA selectivity, and reproducibility across diverse fluorescence microscopy protocols and cell analysis studies.

DNA Binding

Affinity: High AT selectivity

Excellent nuclear staining
Cell Permeability

Penetration: Live cell permeable

No cell permeabilization needed
Fluorescence

Excitation/Emission: 350/461 nm

Bright blue fluorescence
Staining Kinetics

Incubation time: 10-30 minutes

Rapid nuclear staining
Batch Reproducibility

Variation: ±3% between batches

Consistent staining intensity
Storage Stability

3 years under proper storage

Extended dye integrity

Safety Information

Handle with caution - DNA-binding dye. Use specialized safety equipment including safety goggles, gloves, and lab coat. May cause skin and eye irritation. Avoid inhalation of powder. Use only in well-ventilated areas. Protect from light during storage and handling. Follow proper disposal procedures.

Irritant
DNA-Binding Agent
Light Sensitive

Storage & Handling

Store in original containers at -20°C in a dry, dark environment. Keep containers tightly closed and properly labeled. Prepare stock solutions in sterile water or DMSO. Use amber vials or wrap in foil to protect from light. Avoid repeated freeze-thaw cycles. Handle with standard fluorescent dye precautions.

Frozen storage (-20°C)
Protect from light
Sterile water for stocks
Avoid freeze-thaw cycles

Chemical Mechanisms & Reaction Pathways

Hoechst 33342 provides specific DNA binding through minor groove interactions, enabling precise cell biology applications with predictable nuclear staining and quantitative fluorescence for research protocols.

Minor Groove Binding

Specific DNA interaction through hydrogen bonding

Essential for nuclear visualization
Fluorescence Excitation

UV excitation with blue emission fluorescence

Critical for live cell imaging
Cell Permeability

Membrane penetration for live cell staining

Enables real-time analysis
Chromatin Structure

DNA condensation state visualization

Supporting cell cycle studies

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global cell biology laboratory access with complete documentation packages supporting international standards and fluorescent dye method validations.

Research Grade Standards

High purity specifications for fluorescence applications

ISO Standards

International standard compliance for research reagent specifications

Fluorescence Grade

Fluorescence grade specifications for imaging studies

Global Compliance

International cell biology research regulation compliance

Method Validation

Supported fluorescent staining method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's cell biology team provides comprehensive method support, troubleshooting assistance, and fluorescence services to optimize Hoechst 33342 performance in your specific research applications.

Method Development
  • Staining optimization support
  • Imaging protocol guidance
  • Fluorescence methods
  • Custom staining procedures
Research Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Fluorescence testing
  • Cell biology method validation support
Technical Support
  • Fluorescence troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our Hoechst 33342 production emphasizes environmental responsibility through sustainable fluorescent dye synthesis practices, waste minimization, and comprehensive environmental impact management for cell biology research operations.

Waste Minimization

Optimized fluorescent dye synthesis with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient dye processing with emission controls

Safe Disposal

Comprehensive guidance for fluorescent dye waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art fluorescent dye facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade Hoechst 33342 quality and performance across all production batches.

Production Process

Advanced fluorescent dye synthesis and purification in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including fluorescence spectroscopy and HPLC

Hoechst content verification and optical purity analysis
Quality Systems

ISO 9001:2015 quality management with fluorescence laboratory accreditation

Continuous improvement and method validation
Packaging Control

Light-protected containers with moisture-controlled packaging

Photostability protection and contamination prevention

Market Applications & Performance Data

Comprehensive cell biology research data demonstrating Hoechst 33342 effectiveness across diverse applications with quantified performance metrics and fluorescent staining method validations.

Research Laboratories
Purity: >98% fluorescent dye content Performance: Bright nuclear staining Stability: 24+ months light-protected storage
Academic Institutions
Publication Support: 25000+ cited imaging studies Reproducibility: >99% inter-lab agreement Innovation: Custom fluorescent solutions
Biotechnology Companies
Reliability: 99.6% batch consistency Quality Assurance: Validated fluorescence performance Efficiency: Optimized staining protocols

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds research specifications with ultra-high purity and exceptional fluorescence performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Cell Biology Expertise

Dedicated cell biology team provides fluorescence optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed fluorescence performance data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services