HEPES Buffer
Biotechnology Biological Buffer
Biological Buffers

HEPES Buffer

High-purity HEPES buffer essential for biotechnology applications. Provides stable pH control for cell culture systems, protein studies, and biochemical procedures requiring physiological conditions and minimal interference with biological processes.

  • High-Purity Biological Buffer
  • Stable pH Control
  • Cell Culture Compatible
  • Protein Studies
  • Physiological Conditions
  • Minimal Interference
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Technical Specifications

Chemical Name: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
Molecular Formula: C₈H₁₈N₂O₄S
Molecular Weight: 238.31 g/mol
CAS Number: 7365-45-9
Purity: ≥99.5% (Titration)
Physical Form: White crystalline powder
pH Range: 6.8-8.2 (buffering range)
pKa (25°C): 7.55
Solubility: >1 M in water
Temperature Coefficient: -0.014 pH/°C
Storage Temperature: Room temperature (dry place)
Packaging Options: 25g, 100g, 500g, 1kg, 5kg

Applications

Cell Culture Media
Protein Biochemistry
Enzyme Assays
pH Buffering
Protein Purification
Educational Research
Molecular Biology
Live Cell Imaging
Plant Cell Culture
Electrophoresis
Drug Discovery
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized HEPES buffer grades tailored for specific cell culture and biochemical applications, ensuring optimal pH stability and regulatory compliance across diverse biotechnology requirements.

Research Grade
Purity: ≥99.5% (Titration) Heavy metals: <10 ppm pH stability: Excellent Application: General research
Cell Culture Grade
Purity: ≥99.8% (Titration) Sterility: Assured Endotoxin: <0.1 EU/mg Application: Cell culture
Ultra-Pure Grade
Purity: ≥99.9% (Titration) Heavy metals: <5 ppm DNase/RNase: None detected Application: Sensitive assays
Pharmaceutical Grade
Purity: ≥99.5% (USP) GMP: Compliant Validation: Pharmaceutical Application: Drug formulation

Quality Standards

DRAVYOM's HEPES Buffer is manufactured under stringent quality control protocols, meeting international buffer standards including research specifications and cell culture requirements. Our buffer production ensures consistent pH performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Research Grade Specifications
≥99.5% Purity Verified (Titration)
pH Stability Testing
pKa Verification
Buffer Capacity Testing
Certificate of Analysis
Moisture-Controlled Storage

Advanced Chemical Properties & Performance

HEPES Buffer exhibits exceptional buffering properties essential for cell culture and biochemical applications. Its high purity and physiological compatibility ensure reliable performance in demanding cell biology protocols, enzyme assay studies, and molecular biology procedures.

Buffer Properties
Molecular Weight: 238.31 g/mol
Chemical Formula: C₈H₁₈N₂O₄S
Buffer Type: Zwitterionic biological buffer
pKa (25°C): 7.55
Physical Properties
Appearance: White crystalline powder
Solubility (H₂O): ≥ 150 mg/mL
pH (1% solution): 7.2 - 7.6
Temperature Coefficient: -0.014 pH units/°C
Buffering Performance
Effective pH Range: 6.8 - 8.2
Buffer Capacity: High at physiological pH
Metal Chelation: Minimal interference
Osmolality: ~126 mOsm/kg (15 mM)
Purity Specifications
Assay (by titration): ≥ 99.5%
Water Content: ≤ 1.0%
Heavy Metals: ≤ 5 ppm
Endotoxin: ≤ 0.1 EU/mg
Stability Properties
Shelf Life: 5 years (room temperature)
Solution Stability: Stable at 4°C for months
pH Stability: Stable pH 5-10
Photostability: Stable under laboratory lighting

Performance Characteristics

Detailed performance metrics demonstrate HEPES Buffer superiority in biological applications with exceptional buffering capacity, physiological compatibility, and reproducibility across diverse cell culture protocols and biochemical procedures.

Buffer Capacity

Effective range: pH 6.8-8.2

Optimal physiological buffering
Cell Compatibility

Cell viability: >95%

Non-toxic to cells
Temperature Coefficient

ΔpH/ΔT: -0.014 pH units/°C

Minimal temperature dependence
Solubility

Water solubility: ≥150 mg/mL

Excellent aqueous solubility
Batch Reproducibility

Variation: ±0.05 pH units

Consistent buffering performance
Storage Stability

5 years under proper storage

Extended buffer integrity

Safety Information

Generally safe biological buffer for research use. Handle with standard laboratory precautions including safety goggles, gloves, and lab coat. Non-toxic and biodegradable compound. May cause dust irritation. Use standard powder handling procedures. Safe for cell culture applications.

Generally Safe
Biodegradable
Cell Culture Grade

Storage & Handling

Store in original containers at room temperature in a dry environment. Keep containers tightly closed to prevent moisture absorption. Avoid contamination with metals. Use standard powder handling procedures. Solutions can be stored at 4°C for months. Autoclave sterilizable for sterile applications.

Room temperature storage
Protect from moisture
Autoclave sterilizable
Stable in solution

Chemical Mechanisms & Reaction Pathways

HEPES buffer provides precise pH control through zwitterionic buffering, enabling optimal biochemistry applications with predictable physiological conditions and quantitative pH stability for research protocols.

Zwitterionic Buffering

Dual ionic character for optimal buffering capacity

Essential for pH stability
Henderson-Hasselbalch

pH equilibrium maintenance through acid-base chemistry

Critical for biological systems
Protein Stabilization

Conformational protection during biochemical assays

Enables enzyme activity
Cell Culture Compatibility

Physiological buffering for cell viability

Supporting biological research

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global biochemistry laboratory access with complete documentation packages supporting international standards and buffer system method validations.

Research Grade Standards

High purity specifications for biochemistry applications

ISO Standards

International standard compliance for research reagent specifications

Buffer Grade

Buffer grade specifications for biological studies

Global Compliance

International biochemistry research regulation compliance

Method Validation

Supported buffer system method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's biochemistry team provides comprehensive method support, troubleshooting assistance, and buffer services to optimize HEPES performance in your specific research applications.

Method Development
  • Buffer preparation optimization support
  • pH adjustment protocol guidance
  • Buffer chemistry methods
  • Custom buffer procedures
Research Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Buffer capacity testing
  • Biochemistry method validation support
Technical Support
  • Buffer troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our HEPES buffer production emphasizes environmental responsibility through sustainable buffer synthesis practices, waste minimization, and comprehensive environmental impact management for biochemistry research operations.

Waste Minimization

Optimized buffer synthesis with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient buffer processing with emission controls

Safe Disposal

Comprehensive guidance for buffer waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art buffer facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade HEPES quality and performance across all production batches.

Production Process

Advanced buffer synthesis and purification in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including pH measurement and titration

HEPES content verification and buffer capacity analysis
Quality Systems

ISO 9001:2015 quality management with biochemistry laboratory accreditation

Continuous improvement and method validation
Packaging Control

Moisture-resistant containers with humidity-controlled packaging

Hygroscopic protection and contamination prevention

Market Applications & Performance Data

Comprehensive biochemistry research data demonstrating HEPES effectiveness across diverse applications with quantified performance metrics and buffer system method validations.

Research Laboratories
Purity: >99.5% buffer content Performance: Stable pH buffering Stability: 24+ months ambient storage
Academic Institutions
Publication Support: 50000+ cited protocols Reproducibility: >99% inter-lab agreement Innovation: Custom buffer solutions
Biotechnology Companies
Reliability: 99.8% batch consistency Quality Assurance: Validated buffer performance Efficiency: Optimized buffer protocols

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds research specifications with ultra-high purity and exceptional buffering performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Biochemistry Expertise

Dedicated biochemistry team provides buffer optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed buffer performance data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services