GFP Green Fluorescent Protein
Biotechnology Premium
Fluorescent Protein

GFP Green Fluorescent Protein

High-quality GFP (Green Fluorescent Protein) for molecular biology research, protein labeling, and fluorescence applications. Premium grade fluorescent protein manufactured for research and biotechnology applications.

  • Green Fluorescence Emission
  • Molecular Reporter Protein
  • Protein Labeling Applications
  • Live Cell Imaging Compatible
  • Research Grade Purity
  • Biotechnology Standard
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Technical Specifications

Protein Name: Green Fluorescent Protein
Source: Aequorea victoria (Modified)
Molecular Weight: 26.9 kDa
Physical State: Lyophilized powder / Solution
Excitation Maximum: 395 nm (Minor), 475 nm (Major)
Emission Maximum: 509 nm (Green)
pH Stability: 6.0-8.0 (Optimal 7.4)
Storage Temperature: -20°C (Long-term), 4°C (Working)
Purity: ≥95% (SDS-PAGE)
Activity: ≥80% Fluorescent
Endotoxin Level: <1 EU/mg
Packaging Options: 100μg, 500μg, 1mg vials

Applications

Protein Expression Tracking
Live Cell Imaging
Gene Expression Studies
Flow Cytometry
Protein Fusion Studies
Research Applications
Transfection Efficiency
Protein Localization
Plant Cell Studies
Fluorescence Imaging
Biotechnology Research
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized GFP grades tailored for specific research and biotechnology applications, ensuring optimal fluorescence performance and regulatory compliance across diverse molecular biology applications.

Research Grade
Purity: ≥95% (SDS-PAGE) Activity: ≥80% Fluorescent Endotoxin: <5 EU/mg Application: Basic research, education
High-Performance Grade
Purity: ≥98% (SDS-PAGE) Activity: ≥90% Fluorescent Endotoxin: <1 EU/mg Application: Advanced imaging, microscopy
Ultra-Pure Grade
Purity: ≥99% (SDS-PAGE) Activity: ≥95% Fluorescent Aggregation: <1% Application: High-resolution studies
Clinical Grade
Purity: ≥99.5% (SDS-PAGE) Endotoxin: <0.1 EU/mg Sterility: Tested Application: Clinical research

Quality Standards

DRAVYOM's GFP is manufactured under stringent quality control protocols, meeting international biotechnology standards including ISO, cGMP, and research specifications. Our protein production ensures consistent fluorescence performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
cGMP Compliant Production
≥95% SDS-PAGE Verified Purity
Fluorescence Activity Testing
Protein Folding Validation
Batch-to-Batch Consistency
Certificate of Analysis
Cold Chain Logistics

Advanced Chemical Properties & Performance

GFP (Green Fluorescent Protein) exhibits exceptional fluorescent protein properties essential for cellular imaging and molecular biology applications. Its autofluorescent nature and protein stability ensure reliable performance in demanding live cell imaging protocols and reporter gene studies.

Fluorescent Protein Properties
Molecular Weight: 26.9 kDa
Protein Origin: Aequorea victoria jellyfish
Chromophore: p-Hydroxybenzylidene imidazolinone
Fluorescence Type: Intrinsic autofluorescence
Physical Properties
Appearance: Clear to yellow protein solution
Isoelectric Point: pI 5.9
Excitation λmax: 395 nm, 475 nm
Emission λmax: 509 nm
Protein Performance
Quantum Yield: 0.79
Extinction Coefficient: 55,000 M⁻¹cm⁻¹
Brightness: 43,450 M⁻¹cm⁻¹
Photobleaching: Moderate photostability
Purity Specifications
Protein Purity: ≥ 95% (SDS-PAGE)
Endotoxin Level: ≤ 0.1 EU/μg
Activity: ≥ 90% fluorescent
Aggregation: ≤ 5% oligomers
Stability Properties
Shelf Life: 2 years (-80°C)
Working Solution: Weeks at 4°C
pH Stability: Stable pH 6-9
Temperature Stability: Stable up to 65°C

Performance Characteristics

Detailed performance metrics demonstrate GFP superiority in fluorescent protein applications with exceptional brightness, protein stability, and reproducibility across diverse cellular imaging protocols and reporter gene expression studies.

Fluorescence Brightness

Brightness: 43,450 M⁻¹cm⁻¹

Excellent fluorescent signal
Quantum Yield

Efficiency: 0.79

High fluorescence efficiency
Imaging Performance

Excitation/Emission: 475/509 nm

Optimal for fluorescence microscopy
Temperature Stability

Working range: 4-37°C

Stable at physiological temperatures
Batch Reproducibility

Variation: ±5% between batches

Consistent protein activity
Storage Stability

2 years under proper storage

Extended protein integrity

Safety Information

Generally safe recombinant protein for research use. Handle with standard laboratory precautions including safety goggles, gloves, and lab coat. Non-toxic and biodegradable protein. Avoid contamination and maintain sterile conditions. Use standard protein handling procedures.

Generally Safe
Biodegradable Protein
Standard Lab Precautions

Storage & Handling

Store in original containers at -80°C for long-term storage or -20°C for short-term use. Keep containers tightly closed and maintain sterile conditions. Aliquot upon receipt to avoid repeated freeze-thaw cycles. Working solutions can be stored at 4°C for weeks. Protect from prolonged light exposure.

Ultra-cold storage (-80°C)
Maintain sterile conditions
Aliquot to avoid freeze-thaw
Protect from prolonged light

Chemical Mechanisms & Reaction Pathways

Green Fluorescent Protein provides reporter function through chromophore auto-catalysis, enabling precise molecular biology applications with predictable fluorescence emission and quantitative protein expression for research protocols.

Chromophore Formation

Auto-catalytic cyclization of Ser-Tyr-Gly tripeptide

Essential for fluorescence
Protein Folding

Beta-barrel structure formation protecting chromophore

Critical for stability
Fluorescence Emission

Green light emission upon blue light excitation

Enables visualization
Maturation Process

Oxygen-dependent chromophore oxidation and maturation

Supporting fluorescent development

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global molecular biology laboratory access with complete documentation packages supporting international standards and protein expression method validations.

Research Grade Standards

High purity specifications for molecular biology applications

ISO Standards

International standard compliance for research protein specifications

Protein Research Grade

Protein research grade specifications for expression studies

Global Compliance

International molecular biology research regulation compliance

Method Validation

Supported protein expression method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's molecular biology team provides comprehensive method support, troubleshooting assistance, and protein services to optimize GFP performance in your specific research applications.

Method Development
  • Expression optimization support
  • Protein purification protocol guidance
  • Fluorescence imaging methods
  • Custom expression procedures
Research Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Fluorescence testing
  • Protein method validation support
Technical Support
  • Expression troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our GFP production emphasizes environmental responsibility through sustainable protein production practices, waste minimization, and comprehensive environmental impact management for molecular biology research operations.

Waste Minimization

Optimized protein production with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient bioprocess technology with emission controls

Safe Disposal

Comprehensive guidance for protein waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art bioprocess facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade GFP quality and performance across all production batches.

Production Process

Advanced recombinant protein expression and purification in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including fluorometry and SDS-PAGE

Protein identity verification and fluorescence analysis
Quality Systems

ISO 9001:2015 quality management with bioprocess laboratory accreditation

Continuous improvement and method validation
Packaging Control

Cryoprotectant containers with temperature-controlled packaging

Protein stability protection and contamination prevention

Market Applications & Performance Data

Comprehensive molecular biology research data demonstrating GFP effectiveness across diverse applications with quantified performance metrics and protein expression method validations.

Research Laboratories
Purity: >95% protein content Performance: Strong fluorescence emission Stability: 12+ months at -80°C
Academic Institutions
Publication Support: 40000+ cited studies Reproducibility: >98% inter-lab agreement Innovation: Custom protein solutions
Biotechnology Companies
Reliability: 99.8% batch consistency Quality Assurance: Validated fluorescent performance Efficiency: Optimized expression protocols

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds research specifications with ultra-high purity and exceptional fluorescent performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Protein Expertise

Dedicated protein team provides expression optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed protein performance data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services