Fluorescein Diacetate Cell Viability Dye
Biotechnology Premium
Cell Viability Dye

Fluorescein Diacetate Cell Viability Dye

High-quality Fluorescein Diacetate (FDA) for cell viability assays and live cell imaging. Premium grade fluorescent dye manufactured for accurate cell viability determination and microscopy applications.

  • Cell Viability Indicator
  • Live Cell Imaging Compatible
  • Esterase Activity Detection
  • Non-Toxic to Live Cells
  • Flow Cytometry Compatible
  • Research Grade Quality
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Technical Specifications

Chemical Name: Fluorescein Diacetate (FDA)
Molecular Formula: C₂₄H₁₆O₇
Molecular Weight: 416.38 g/mol
CAS Number: 596-09-8
Purity: ≥98% (HPLC)
Physical Form: Off-white to pale yellow powder
Solubility: DMSO, acetone; slightly soluble in water
Excitation/Emission: 494 nm / 518 nm (after hydrolysis)
Storage Temperature: -20°C (protect from light)
Shelf Life: 3 years at -20°C
Working Concentration: 1-10 μg/mL
Packaging Options: 5mg, 25mg, 100mg, 1g

Applications

Cell Viability Assays
Live Cell Imaging
Enzyme Activity Studies
Flow Cytometry
Metabolic Activity
Educational Research
Fluorescence Microscopy
Drug Screening
Plant Cell Studies
Toxicity Testing
Bioassays
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized fluorescein diacetate grades tailored for specific cell viability and enzyme activity applications, ensuring optimal fluorescent response and regulatory compliance across diverse biotechnology assays.

Research Grade
Purity: ≥98% (HPLC) Fluorescence: High Background: Low Application: General research
Cell Culture Grade
Purity: ≥99% (HPLC) Sterility: Assured Endotoxin: <0.1 EU/mg Application: Cell studies
Ultra-Pure Grade
Purity: ≥99.5% (HPLC) Heavy metals: <10 ppm Moisture: <0.1% Application: Sensitive assays
Pharmaceutical Grade
Purity: ≥98% (USP) GMP: Compliant Validation: Pharmaceutical Application: Drug testing

Quality Standards

DRAVYOM's Fluorescein Diacetate is manufactured under stringent quality control protocols, meeting international fluorescent probe standards including research specifications and cell biology requirements. Our viability reagent production ensures consistent performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Cell Culture Grade Specifications
≥98% Purity Verified (HPLC)
Fluorescence Testing
Enzyme Activity Assessment
Stability Testing
Certificate of Analysis
Ultra-Cold Storage

Advanced Chemical Properties & Performance

Fluorescein Diacetate exhibits exceptional cell viability staining properties essential for live cell assessment applications. Its esterase-dependent activation and fluorescent characteristics ensure reliable performance in demanding cell biology protocols and viability assay studies.

Cell Viability Dye Properties
Molecular Weight: 416.38 g/mol
Chemical Formula: C₂₄H₁₆O₇
Dye Type: Esterase-activated fluorophore
Activation Mechanism: Intracellular esterase cleavage
Physical Properties
Appearance: Yellow crystalline powder
Solubility: DMSO, acetone soluble
Excitation λmax: 495 nm (after activation)
Emission λmax: 519 nm (fluorescein)
Cell Viability Performance
Live Cell Detection: Bright green fluorescence
Dead Cell Signal: No fluorescence
Incubation Time: 5-30 minutes
Working Concentration: 1-10 μg/mL
Purity Specifications
Dye Purity: ≥ 97% (HPLC)
Water Content: ≤ 1%
Heavy Metals: ≤ 10 ppm
Free Fluorescein: ≤ 1%
Stability Properties
Shelf Life: 2 years (-20°C, dark)
Solution Stability: Days at 4°C in DMSO
Light Sensitivity: Protect from light
Hydrolysis Rate: Slow in aqueous solutions

Performance Characteristics

Detailed performance metrics demonstrate Fluorescein Diacetate superiority in cell viability applications with exceptional live cell discrimination, rapid activation kinetics, and reproducibility across diverse cell biology protocols and viability assessment studies.

Viability Detection

Live cell discrimination: >95%

Excellent viability assessment
Activation Kinetics

Fluorescence development: 5-30 min

Rapid esterase activation
Fluorescence Signal

Excitation/Emission: 495/519 nm

Bright green fluorescence
Temperature Stability

Working range: 4-37°C

Stable at physiological temperatures
Batch Reproducibility

Variation: ±3% between batches

Consistent staining performance
Storage Stability

2 years under proper storage

Extended dye integrity

Safety Information

Generally safe fluorescent dye for research use. Handle with standard laboratory precautions including safety goggles, gloves, and lab coat. May cause mild irritation to eyes and skin. Avoid inhalation of powder. Use in well-ventilated areas. Protect from light during storage and handling.

Generally Safe
Mild Irritant
Light Sensitive

Storage & Handling

Store in original containers at -20°C in a dry, dark environment. Keep containers tightly closed to prevent moisture absorption and light exposure. Prepare stock solutions in DMSO and store at -20°C. Use amber vials or wrap in foil to protect from light. Avoid repeated freeze-thaw cycles.

Frozen storage (-20°C)
Protect from light
DMSO for stock solutions
Avoid freeze-thaw cycles

Chemical Mechanisms & Reaction Pathways

Fluorescein diacetate provides cell viability assessment through esterase-mediated hydrolysis, enabling precise cell biology applications with predictable fluorescence activation and quantitative live cell detection for research protocols.

Esterase Hydrolysis

Intracellular esterase cleavage of acetate groups

Essential for fluorescence activation
Fluorescence Generation

Conversion to fluorescent fluorescein upon hydrolysis

Critical for detection
Membrane Permeability

Non-fluorescent precursor crosses intact membranes

Enables live cell assays
Retention Mechanism

Fluorescein retention in viable cells with intact membranes

Supporting viability assessment

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global cell biology laboratory access with complete documentation packages supporting international standards and cell viability method validations.

Research Grade Standards

High purity specifications for cell biology applications

ISO Standards

International standard compliance for research reagent specifications

Cell Biology Grade

Cell biology grade specifications for viability studies

Global Compliance

International cell biology research regulation compliance

Method Validation

Supported viability assay method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's cell biology team provides comprehensive method support, troubleshooting assistance, and viability services to optimize fluorescein diacetate performance in your specific research applications.

Method Development
  • Viability assay optimization support
  • Cell staining protocol guidance
  • Live cell imaging methods
  • Custom assay procedures
Research Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Fluorescence testing
  • Cell biology method validation support
Technical Support
  • Assay troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our fluorescein diacetate production emphasizes environmental responsibility through sustainable synthesis practices, waste minimization, and comprehensive environmental impact management for cell biology research operations.

Waste Minimization

Optimized fluorescent dye synthesis with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient organic synthesis with emission controls

Safe Disposal

Comprehensive guidance for fluorescent dye waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art fluorescent dye facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade fluorescein diacetate quality and performance across all production batches.

Production Process

Advanced fluorescent dye synthesis and purification in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including fluorometry and HPLC

Fluorescent activation verification and purity analysis
Quality Systems

ISO 9001:2015 quality management with cell biology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Light-protected containers with moisture-controlled packaging

Photostability protection and contamination prevention

Market Applications & Performance Data

Comprehensive cell biology research data demonstrating fluorescein diacetate effectiveness across diverse applications with quantified performance metrics and viability assay method validations.

Research Laboratories
Purity: >98% ester content Performance: Rapid cellular uptake Stability: 24+ months at -20°C
Academic Institutions
Publication Support: 8000+ cited protocols Reproducibility: >97% inter-lab agreement Innovation: Custom viability solutions
Biotechnology Companies
Reliability: 99.6% batch consistency Quality Assurance: Validated viability performance Efficiency: Optimized assay protocols

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds research specifications with ultra-high purity and exceptional fluorescent performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Cell Biology Expertise

Dedicated cell biology team provides viability optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed viability performance data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services