Ethidium Bromide DNA Stain
Biotechnology Safety Required
DNA Visualization

Ethidium Bromide (DNA Stain)

Traditional ethidium bromide DNA stain for nucleic acid visualization in electrophoresis applications. Note: Safety considerations apply - safer alternatives are now available for routine use. Proper handling and disposal protocols essential.

  • Traditional DNA Stain
  • Nucleic Acid Visualization
  • Electrophoresis Applications
  • Safety Protocols Required
  • Research Grade
  • Safer Alternatives Available
Request Quote Safety Information

Technical Specifications

Chemical Name: 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide
Molecular Formula: C₂₁H₂₀BrN₃
Molecular Weight: 394.31 g/mol
CAS Number: 1239-45-8
Purity: ≥95% (HPLC)
Physical Form: Dark red crystalline powder
Solubility: 10 mg/mL in water
Excitation/Emission: 518 nm / 605 nm
Storage Temperature: 2-8°C (protect from light)
Shelf Life: 3 years at 2-8°C
Light Sensitivity: Light sensitive (store in dark)
Packaging Options: 10mg, 25mg, 100mg, 1g

Applications

DNA Staining
Gel Electrophoresis
Agarose Gel Staining
Fluorescent Microscopy
DNA Quantification
Educational Research
Molecular Biology
PCR Product Analysis
Plant Genomics
DNA Visualization
Research Applications
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized ethidium bromide grades tailored for specific DNA staining applications, ensuring optimal fluorescence and regulatory compliance across diverse molecular biology requirements.

Research Grade
Purity: ≥95% (HPLC) Fluorescence: High DNA binding: Optimized Application: General research
Molecular Biology Grade
Purity: ≥98% (HPLC) DNase/RNase: None detected Endotoxin: <0.1 EU/mg Application: DNA work
Ultra-Pure Grade
Purity: ≥99% (HPLC) Heavy metals: <10 ppm Background: Minimal Application: Sensitive assays
Educational Grade
Purity: ≥95% (HPLC) Safety: Enhanced protocols Documentation: Complete Application: Teaching labs

Quality Standards

DRAVYOM's Ethidium Bromide is manufactured under stringent quality control protocols, meeting international fluorescent dye standards including research specifications and safety requirements. Our staining reagent production ensures consistent fluorescence and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Research Grade Specifications
≥95% Purity Verified (HPLC)
Fluorescence Testing
DNA Binding Assessment
Safety Testing
Certificate of Analysis
Light-Protected Storage

Advanced Chemical Properties & Performance

Ethidium Bromide exhibits exceptional nucleic acid staining properties essential for DNA/RNA visualization applications. Its intercalating ability and fluorescent characteristics ensure reliable performance in demanding gel electrophoresis protocols and nucleic acid detection studies.

Intercalating Agent Properties
Molecular Weight: 394.31 g/mol
Chemical Formula: C₂₁H₂₀BrN₃
Staining Type: DNA/RNA intercalating dye
Binding Mode: Between base pairs
Physical Properties
Appearance: Dark red crystalline powder
Solubility: Water soluble (10 mg/mL)
Excitation λmax: 518 nm
Emission λmax: 605 nm
Fluorescence Performance
DNA Enhancement: 21-fold fluorescence increase
RNA Enhancement: 25-fold fluorescence increase
Detection Limit: 1 ng DNA
Working Concentration: 0.5 μg/mL
Purity Specifications
Dye Purity: ≥ 95% (HPLC)
Water Content: ≤ 3%
Heavy Metals: ≤ 10 ppm
Related Substances: ≤ 2% each
Stability Properties
Shelf Life: 3 years (4°C, dark)
Solution Stability: Weeks at 4°C in dark
Light Sensitivity: Protect from light
pH Stability: Stable pH 7-9

Performance Characteristics

Detailed performance metrics demonstrate Ethidium Bromide superiority in nucleic acid visualization applications with exceptional fluorescence enhancement, DNA/RNA detection sensitivity, and reproducibility across diverse gel electrophoresis protocols and molecular biology studies.

DNA Staining

Fluorescence enhancement: 21-fold

Excellent DNA visualization
Detection Sensitivity

Minimum detection: 1 ng DNA

High sensitivity visualization
Fluorescence

Excitation/Emission: 518/605 nm

Optimal UV transillumination
Temperature Stability

Working range: 4-25°C

Stable at laboratory temperatures
Batch Reproducibility

Variation: ±5% between batches

Consistent staining intensity
Storage Stability

3 years under proper storage

Extended dye integrity

Safety Information

HIGHLY TOXIC mutagenic compound requiring extreme caution. Handle with specialized safety equipment including double gloves, safety goggles, and lab coat. Suspected carcinogen and mutagen. Use only in fume hood. Dispose as hazardous waste. Avoid skin contact and inhalation. Emergency procedures required.

HIGHLY TOXIC
Mutagen/Carcinogen
EXTREME CAUTION

Storage & Handling

Store in original containers at 4°C in a dry, dark environment with restricted access. Keep containers tightly closed and properly labeled as hazardous. Use dedicated storage area with containment. Handle only with extreme safety precautions. Prepare solutions in fume hood. Dispose according to hazardous waste protocols.

Refrigerated storage (4°C)
Protect from light
Restricted access storage
Hazardous waste disposal

Chemical Mechanisms & Reaction Pathways

Ethidium bromide provides DNA intercalation through planar aromatic insertion, enabling precise molecular biology applications with predictable fluorescence enhancement and quantitative nucleic acid detection for research protocols.

DNA Intercalation

Planar aromatic ring insertion between DNA base pairs

Essential for visualization
Fluorescence Enhancement

Significant fluorescence increase upon DNA binding

Critical for detection
Base Pair Unwinding

DNA double helix unwinding and lengthening

Enables gel visualization
Electrostatic Interaction

Cationic dye interaction with DNA phosphate backbone

Supporting binding stability

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global molecular biology laboratory access with complete documentation packages supporting international standards and DNA staining method validations.

Research Grade Standards

High purity specifications for molecular biology applications

ISO Standards

International standard compliance for research reagent specifications

Molecular Biology Grade

Molecular biology grade specifications for DNA studies

Global Compliance

International molecular biology research regulation compliance

Method Validation

Supported DNA staining method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's molecular biology team provides comprehensive method support, troubleshooting assistance, and staining services to optimize ethidium bromide performance in your specific research applications.

Method Development
  • Staining optimization support
  • Gel electrophoresis protocol guidance
  • DNA visualization methods
  • Custom staining procedures
Research Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Fluorescence testing
  • Molecular biology method validation support
Technical Support
  • Staining troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our ethidium bromide production emphasizes environmental responsibility through sustainable synthesis practices, waste minimization, and comprehensive environmental impact management for molecular biology research operations.

Waste Minimization

Optimized dye synthesis with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient organic synthesis with emission controls

Safe Disposal

Comprehensive guidance for hazardous dye waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art organic dye facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade ethidium bromide quality and performance across all production batches.

Production Process

Advanced dye synthesis and purification in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including fluorescence and HPLC

Fluorescent intensity verification and purity analysis
Quality Systems

ISO 9001:2015 quality management with molecular biology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Light-protected containers with moisture-controlled packaging

Photostability protection and contamination prevention

Market Applications & Performance Data

Comprehensive molecular biology research data demonstrating ethidium bromide effectiveness across diverse applications with quantified performance metrics and DNA staining method validations.

Research Laboratories
Purity: >95% dye content Performance: Strong fluorescence enhancement Stability: 36+ months light-protected
Academic Institutions
Publication Support: 30000+ cited protocols Reproducibility: >96% inter-lab agreement Innovation: Safer alternative guidance
Biotechnology Companies
Reliability: 99.3% batch consistency Quality Assurance: Validated fluorescent performance Efficiency: Optimized staining protocols

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds research specifications with ultra-high purity and exceptional fluorescent performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Molecular Biology Expertise

Dedicated molecular biology team provides staining optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed fluorescent performance data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services