EMB Agar
Biotechnology Selective Media
Culture Media

EMB Agar (Culture Media Component)

High-quality EMB (Eosin Methylene Blue) Agar essential for microbiology applications. Provides selective and differential culture media for bacterial isolation and identification with clear colony morphology distinction and gram-negative bacterial selection.

  • High-Quality Selective Media
  • Differential Properties
  • Bacterial Isolation
  • Colony Morphology
  • Gram-Negative Selection
  • Microbiology Grade
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Technical Specifications

Media Type: Eosin Methylene Blue (EMB) Agar
Selectivity: Gram-negative bacteria
Differential Properties: Lactose fermentation
pH Range: 7.1 ± 0.2 at 25°C
Physical Form: Light amber powder
Solubility: 36 g/L in distilled water
Sterilization: 121°C for 15 minutes
Storage Temperature: 2-25°C (dry place)
Shelf Life: 3 years at room temperature
Final pH: 7.1 ± 0.2 after autoclaving
Plate Appearance: Clear to slightly hazy
Packaging Options: 100g, 500g, 1kg containers

Applications

Bacterial Isolation
Enterobacteria Selection
Lactose Fermentation Test
E. coli Detection
Clinical Microbiology
Educational Research
Quality Control
Food Microbiology
Environmental Testing
Water Analysis
Pharmaceutical Testing
USP Testing

Industry-Specific Grades

DRAVYOM offers specialized EMB Agar grades tailored for specific microbiological applications, ensuring optimal selectivity and regulatory compliance across diverse laboratory testing requirements.

Research Grade
pH: 7.1 ± 0.2 Selectivity: High Sterility: Assured Application: General microbiology
Clinical Grade
pH: 7.1 ± 0.1 Performance: Validated QC: Enhanced Application: Clinical diagnostics
Pharmaceutical Grade
pH: 7.1 ± 0.1 USP: Compliant Endotoxin: <0.25 EU/mL Application: Drug testing
Food Grade
pH: 7.1 ± 0.2 Sensitivity: Enhanced FDA: Approved methods Application: Food safety

Quality Standards

DRAVYOM's EMB Agar is manufactured under stringent quality control protocols, meeting international microbiological standards including USP, EP, and ISO specifications. Our media production ensures consistent performance and regulatory compliance.

ISO 11133:2014 Certified
USP <61> Compliant
pH 7.1 ± 0.2 Verified
Performance Testing
Selectivity Assessment
Sterility Testing
Certificate of Analysis
Temperature-Controlled Storage

Advanced Chemical Properties & Performance

EMB Agar (Eosin Methylene Blue Agar) exhibits exceptional selective culture medium properties essential for gram-negative bacteria differentiation. Its chromogenic indicators and nutrient balance ensure reliable performance in demanding bacterial identification protocols and microbiological studies.

Selective Medium Properties
Medium Type: Selective-differential agar
Selectivity: Gram-negative bacteria
Indicators: Eosin Y, Methylene Blue
pH Range: 7.1-7.5
Physical Properties
Appearance: Dark purple to black agar plates
Gel Strength: 15-18 g/L agar
Solubility: Complete in hot water
Sterilization: 121°C for 15 minutes
Microbiological Performance
E. coli Growth: Green metallic sheen
Enterobacter Growth: Pink to purple colonies
Gram-positive Inhibition: Complete or partial
Incubation: 18-24 hours at 37°C
Quality Specifications
Sterility: Sterile after autoclaving
pH Accuracy: 7.2 ± 0.2
Color Intensity: Consistent dark purple
Moisture Content: ≤ 7%
Stability Properties
Shelf Life: 3 years (dry powder)
Prepared Media: 2-3 weeks refrigerated
Storage Temperature: Room temperature, dry
Light Sensitivity: Protect from direct sunlight

Performance Characteristics

Detailed performance metrics demonstrate EMB Agar superiority in bacterial differentiation applications with exceptional selectivity, colony morphology differentiation, and reproducibility across diverse microbiology protocols and enteric bacteria identification studies.

Selectivity

Gram-negative recovery: >90%

Excellent selective pressure
Differentiation

E. coli identification: >95% accuracy

Clear metallic sheen formation
Inhibition

Gram-positive suppression: >85%

Effective selective inhibition
Temperature Stability

Working range: 35-42°C

Stable at incubation temperatures
Batch Reproducibility

Variation: ±2% between batches

Consistent growth patterns
Stability Performance

3 years under proper storage

Extended medium integrity

Safety Information

Generally safe culture medium for laboratory use. Handle with standard microbiological safety precautions including biosafety cabinet when working with bacterial cultures. Follow aseptic technique. Contains methylene blue and eosin dyes. Use appropriate personal protective equipment.

Generally Safe
Aseptic Technique Required
Biosafety Precautions

Storage & Handling

Store in original containers at room temperature in a dry, dark environment. Keep containers tightly closed to prevent moisture absorption and contamination. Prepared plates should be stored refrigerated and used within 2-3 weeks. Maintain sterile conditions during preparation and handling.

Room temperature storage
Protect from light
Maintain sterility
Prepared plates refrigerated

Chemical Mechanisms & Reaction Pathways

EMB Agar provides selective bacterial isolation through differential inhibition mechanisms, enabling precise microbiology applications with predictable growth characteristics and quantitative bacterial differentiation for research protocols.

Selective Inhibition

Methylene blue and eosin Y inhibit gram-positive bacteria

Essential for gram-negative selection
Differential Staining

Lactose fermentation creates metallic green sheen

Critical for E. coli identification
pH Indicators

Color change indicators for metabolic activity detection

Enables bacterial differentiation
Nutrient Balance

Optimized nutrient composition for bacterial growth

Supporting selective cultivation

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global microbiology laboratory access with complete documentation packages supporting international standards and bacterial isolation method validations.

Research Grade Standards

High quality specifications for microbiology applications

ISO Standards

International standard compliance for research media specifications

Microbiology Grade

Microbiology grade specifications for bacterial studies

Global Compliance

International microbiology research regulation compliance

Method Validation

Supported bacterial isolation method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's microbiology team provides comprehensive method support, troubleshooting assistance, and media services to optimize EMB Agar performance in your specific research applications.

Method Development
  • Media preparation optimization support
  • Bacterial isolation protocol guidance
  • Selective cultivation methods
  • Custom media procedures
Research Services
  • Certificate of Analysis verification
  • Custom sterility testing
  • Growth performance evaluation
  • Microbiology method validation support
Technical Support
  • Media troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our EMB Agar production emphasizes environmental responsibility through sustainable media preparation practices, waste minimization, and comprehensive environmental impact management for microbiology research operations.

Waste Minimization

Optimized media preparation with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient media processing with emission controls

Safe Disposal

Comprehensive guidance for media waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art media manufacturing facility employs advanced sterilization technology and continuous monitoring systems to ensure consistent research-grade EMB Agar quality and performance across all production batches.

Production Process

Advanced media preparation and sterilization in controlled environment

Multi-stage sterilization for research grade quality
Quality Testing

Comprehensive microbiological testing including sterility and growth promotion

Bacterial growth verification and selectivity testing
Quality Systems

ISO 9001:2015 quality management with microbiology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Sterile containers with aseptic packaging conditions

Sterility protection and contamination prevention

Market Applications & Performance Data

Comprehensive microbiology research data demonstrating EMB Agar effectiveness across diverse applications with quantified performance metrics and bacterial isolation method validations.

Research Laboratories
Sterility: Validated aseptic production Selectivity: >95% gram-negative recovery Stability: 12+ months refrigerated
Academic Institutions
Publication Support: 5000+ cited protocols Reproducibility: >98% inter-lab agreement Innovation: Custom media solutions
Biotechnology Companies
Reliability: 99.8% batch consistency Quality Assurance: Validated selective performance Efficiency: Optimized isolation protocols

DRAVYOM Competitive Advantages

Superior Quality

Consistently exceeds research specifications with ultra-high sterility and exceptional selective performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Microbiology Expertise

Dedicated microbiology team provides media optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed growth performance data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services