EDTA Chelating Agent
Biotechnology Chelating
Biotechnology Reagents

EDTA (Chelating Agent)

High-purity EDTA (Ethylenediaminetetraacetic acid) chelating agent essential for biotechnology applications. Provides reliable metal ion chelation for molecular biology procedures, cell culture applications, and biochemical research requiring metal-free conditions.

  • High-Purity Chelating Agent
  • Reliable Metal Ion Chelation
  • Molecular Biology Grade
  • Cell Culture Compatible
  • Biochemical Research Grade
  • Laboratory Validated
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Technical Specifications

Chemical Name: Ethylenediaminetetraacetic acid
Molecular Formula: C₁₀H₁₆N₂O₈
Molecular Weight: 292.24 g/mol
CAS Number: 60-00-4
Physical Form: White crystalline powder
Purity (Complexometric): ≥99.0%
Solubility: 0.2 g/L (water at 20°C)
pH (1% solution): 4.0-6.0
Storage Temperature: Room temperature (Dry place)
Shelf Life: 5 years at room temperature
Chelation Capacity: High (multivalent metals)
Packaging Options: 100g, 500g, 1kg, 5kg

Applications

Metal Ion Chelation
DNA/RNA Extraction
Buffer Preparation
Enzyme Protection
PCR Applications
Educational Research
Cell Culture
Protein Purification
Plant Tissue Culture
Analytical Chemistry
Electroplating
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized EDTA grades tailored for specific chelation and molecular biology applications, ensuring optimal metal binding capacity and regulatory compliance across diverse research applications.

Research Grade
Purity: ≥99.0% Chelation: High capacity Heavy metals: <50 ppm Application: General research
Molecular Biology Grade
Purity: ≥99.5% DNase/RNase: None detected Endotoxin: <0.1 EU/mg Application: DNA/RNA work
Ultra-Pure Grade
Purity: ≥99.9% Heavy metals: <10 ppm Trace metals: Minimal Application: Sensitive assays
Analytical Grade
Purity: ≥99.0% (ACS) Traceability: Certified Standards: ACS compliant Application: Analytical chemistry

Quality Standards

DRAVYOM's EDTA is manufactured under stringent quality control protocols, meeting international chelation standards including ACS, USP, and research specifications. Our chelating agent production ensures consistent binding capacity and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
ACS Reagent Grade Specifications
≥99.0% Purity Verified
Chelation Capacity Testing
Metal Content Analysis
Stability Testing
Certificate of Analysis
Moisture-Controlled Storage

Advanced Chemical Properties & Performance

EDTA (Ethylenediaminetetraacetic acid) exhibits exceptional chelating agent properties essential for biochemical applications. Its metal-binding capacity and pH buffering characteristics ensure reliable performance in demanding enzymatic protocols and protein purification studies.

Chelating Properties
Molecular Weight: 372.24 Da (disodium salt)
Chelating Groups: Hexadentate ligand
Metal Binding: Ca²⁺, Mg²⁺, Mn²⁺, Fe³⁺
Stoichiometry: 1:1 metal-ligand complex
Physical Properties
Appearance: White crystalline powder
Solubility (25°C): 1080 g/L in water
pH (1% solution): 4.3-4.7
Dissociation Constants: pKa 2.0, 2.7, 6.2, 10.3
Biochemical Performance
Metal Sequestration: Strong chelation ability
Enzyme Inhibition: Metal-dependent enzymes
DNA Protection: Prevents nuclease activity
Protein Stabilization: Prevents metal-catalyzed oxidation
Purity Specifications
Assay (dry basis): ≥ 99%
Heavy Metals: ≤ 5 ppm
Chloride: ≤ 0.005%
Insoluble Matter: ≤ 0.01%
Stability Properties
Shelf Life: 5 years (room temperature)
Solution Stability: Indefinite when sterile
pH Stability: Stable across wide pH range
Temperature Stability: Stable up to 100°C

Performance Characteristics

Detailed performance metrics demonstrate EDTA superiority in metal chelation applications with exceptional binding capacity, enzyme inhibition, and reproducibility across diverse biochemical protocols and protein purification studies.

Metal Binding

Chelation: Strong hexadentate binding

Excellent metal sequestration
Solubility Performance

Water solubility: 1080 g/L at 25°C

Excellent aqueous dissolution
Enzyme Inhibition

Nuclease inhibition: Complete at 1-10 mM

Effective enzyme protection
Temperature Stability

Working range: 4-100°C

Stable across wide temperature range
Batch Reproducibility

Variation: ±1% between batches

Consistent chelation performance
Stability Performance

5 years under proper storage

Extended chelator integrity

Safety Information

Generally safe chelating agent for research use. Handle with standard laboratory precautions including safety goggles, gloves, and lab coat. May cause mild irritation to eyes and respiratory tract. Avoid inhalation of dust and direct contact with eyes. Non-toxic at normal usage levels.

Generally Safe
Mild Eye Irritant
Standard Lab Precautions

Storage & Handling

Store in original containers at room temperature in a dry environment. Keep containers tightly closed to prevent moisture absorption. Solutions can be sterilized and stored at room temperature for extended periods. Stable under normal laboratory conditions.

Room temperature storage
Dry environment required
Original containers tightly closed
Stable under normal conditions

Chemical Mechanisms & Reaction Pathways

EDTA (Ethylenediaminetetraacetic acid) provides exceptional metal ion chelation through hexadentate coordination, enabling precise molecular biology applications with predictable metal sequestration and quantitative ion removal for research protocols.

Metal Chelation

Hexadentate coordination with divalent metal ions

Essential for ion removal
Complex Formation

Stable 1:1 metal-EDTA complex formation

Critical for chelation efficiency
Enzyme Protection

Prevents metal-catalyzed enzyme degradation

Enables protein stability
pH Dependence

pH-dependent chelation strength and selectivity

Supporting optimized conditions

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global molecular biology laboratory access with complete documentation packages supporting international standards and chelation method validations.

Research Grade Standards

High purity specifications for molecular biology applications

ISO Standards

International standard compliance for research reagent specifications

Chelation Grade

Chelation grade specifications for biochemical studies

Global Compliance

International molecular biology research regulation compliance

Method Validation

Supported chelation method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's biochemistry team provides comprehensive method support, troubleshooting assistance, and chelation services to optimize EDTA performance in your specific research applications.

Method Development
  • Chelation optimization support
  • Metal removal protocol guidance
  • Biochemical stabilization methods
  • Custom chelation procedures
Research Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Chelation efficiency testing
  • Biochemistry method validation support
Technical Support
  • Chelation troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our EDTA production emphasizes environmental responsibility through sustainable synthesis practices, waste minimization, and comprehensive environmental impact management for biochemistry research operations.

Waste Minimization

Optimized chelator synthesis with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient organic synthesis with emission controls

Safe Disposal

Comprehensive guidance for chelator waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art organic synthesis facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade EDTA quality and performance across all production batches.

Production Process

Advanced organic synthesis and purification in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including complexometric titration

Chelation capacity verification and purity analysis
Quality Systems

ISO 9001:2015 quality management with biochemistry laboratory accreditation

Continuous improvement and method validation
Packaging Control

Moisture-resistant containers with sealed packaging

Hygroscopic protection and contamination prevention

Market Applications & Performance Data

Comprehensive biochemistry research data demonstrating EDTA effectiveness across diverse applications with quantified performance metrics and chelation method validations.

Research Laboratories
Purity: >99% chelator content Efficiency: Complete metal sequestration Stability: 36+ months ambient storage
Academic Institutions
Publication Support: 20000+ cited studies Reproducibility: >99% inter-lab agreement Innovation: Custom chelation solutions
Biotechnology Companies
Reliability: 99.9% batch consistency Quality Assurance: Validated chelation performance Efficiency: Optimized biochemical protocols

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds research specifications with ultra-high purity and exceptional chelation performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Biochemistry Expertise

Dedicated biochemistry team provides chelation optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed chelation performance data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services