EDC Peptide Coupling Reagent
Biotechnology Premium
Carbodiimide Coupling Agent

EDC Peptide Coupling Reagent

High-quality EDC (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide) for peptide synthesis and protein conjugation. Premium grade carbodiimide coupling agent manufactured for biotechnology applications and bioconjugation.

  • Carbodiimide Mechanism
  • Protein Conjugation Compatible
  • Amide Bond Formation
  • Bioconjugation Applications
  • High Coupling Efficiency
  • Research Grade Quality
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Technical Specifications

Chemical Name: 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide
Molecular Formula: C₈H₁₇N₃ · HCl
Molecular Weight: 191.70 g/mol
CAS Number: 25952-53-8
Physical Form: White to off-white crystalline powder
Purity (HPLC): ≥98%
Solubility: Water (100 mg/mL), DMF, DMSO
pH (5% solution): 4.5-6.5
Storage Temperature: 2-8°C (Store in dry place)
Shelf Life: 3 years at 2-8°C
Reaction Efficiency: High (amide bond formation)
Packaging Options: 1g, 5g, 25g, 100g

Applications

Protein Conjugation
DNA-Protein Crosslinking
Peptide Synthesis
Bioconjugation
Antibody Labeling
Educational Research
Immunochemistry
Enzyme Immobilization
Drug Development
Fluorescent Labeling
Surface Modification
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized EDC grades tailored for specific bioconjugation and coupling applications, ensuring optimal reaction efficiency and regulatory compliance across diverse biotechnology applications.

Research Grade
Purity: ≥98% (HPLC) Coupling: High efficiency Moisture: <0.5% Application: General research
Bioconjugation Grade
Purity: ≥99% (HPLC) Activity: Enhanced Stabilizers: Optimized Application: Protein coupling
Ultra-Pure Grade
Purity: ≥99.5% (HPLC) Endotoxin: <0.1 EU/mg Heavy metals: <10 ppm Application: Sensitive assays
Pharmaceutical Grade
Purity: ≥99% (USP) GMP: Compliant Validation: Pharmaceutical Application: Drug development

Quality Standards

DRAVYOM's EDC is manufactured under stringent quality control protocols, meeting international bioconjugation standards including USP, EP, and research specifications. Our coupling reagent production ensures consistent activity and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Bioconjugation Grade Specifications
≥98% Purity Verified (HPLC)
Coupling Efficiency Testing
Activity Assessment
Stability Testing
Certificate of Analysis
Temperature-Controlled Storage

Advanced Chemical Properties & Performance

EDC (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide) exhibits exceptional coupling reagent properties essential for bioconjugation applications. Its carbodiimide reactivity and water-soluble nature ensure reliable performance in demanding protein conjugation protocols and crosslinking studies.

Coupling Reagent Properties
Molecular Weight: 191.7 Da (HCl salt)
Reagent Type: Zero-length crosslinker
Reactive Groups: Carboxyl to amine coupling
Reaction Type: Amide bond formation
Physical Properties
Appearance: White to off-white crystalline powder
Solubility: Water soluble (>500 mg/mL)
pH Stability: Active pH 4.5-6.5
Hydrolysis Rate: Half-life ~1 hour (pH 7, 25°C)
Coupling Performance
Coupling Efficiency: High carboxyl activation
Reaction Time: 15 minutes - 2 hours
Temperature Range: 4-25°C (optimal)
Stoichiometry: 2-10 fold molar excess
Purity Specifications
Reagent Purity: ≥ 98% (HPLC)
Water Content: ≤ 2%
Heavy Metals: ≤ 10 ppm
Related Substances: ≤ 1% each
Stability Properties
Shelf Life: 3 years (-20°C, dry)
Solution Stability: Hours at 4°C
pH Sensitivity: Hydrolyzes rapidly at pH >7
Storage Requirement: Desiccated, frozen conditions

Performance Characteristics

Detailed performance metrics demonstrate EDC superiority in bioconjugation applications with exceptional coupling efficiency, water solubility, and reproducibility across diverse protein crosslinking protocols and bioconjugation studies.

Coupling Efficiency

Amide formation: >80% yield

High carboxyl-amine coupling
Water Solubility

Solubility: >500 mg/mL

Excellent aqueous compatibility
Reaction Kinetics

Activation time: 15 minutes

Rapid carboxyl activation
Temperature Stability

Working range: 4-25°C

Stable at biological temperatures
Batch Reproducibility

Variation: ±3% between batches

Consistent coupling performance
Storage Stability

3 years under proper storage

Extended reagent integrity

Safety Information

Potential irritant requiring careful handling. Handle with standard laboratory safety equipment including safety goggles, gloves, and lab coat. Avoid inhalation of powder and skin contact. May cause irritation to eyes, skin, and respiratory tract. Use in well-ventilated areas.

Potential Irritant
Avoid Skin Contact
Use with Ventilation

Storage & Handling

Store in original containers at -20°C in a dry environment with desiccant. Keep containers tightly closed to prevent moisture absorption. Prepare solutions fresh and use immediately. Protect from light and moisture during storage and handling.

Frozen storage (-20°C)
Desiccant required
Use solutions immediately
Protect from moisture

Chemical Mechanisms & Reaction Pathways

EDC (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide) facilitates efficient bioconjugation through carboxyl-to-amine coupling reactions, enabling precise protein modification applications with predictable coupling efficiency and quantitative bioconjugation for research protocols.

Amide Bond Formation

Zero-length crosslinking between carboxyl and amino groups

Essential for bioconjugation
O-Acylisourea Intermediate

Reactive intermediate formation with carboxyl groups

Critical for coupling efficiency
Protein Modification

Selective conjugation of proteins and biomolecules

Enables bioconjugation applications
Water Solubility

Enhanced aqueous solubility for biological applications

Supporting physiological conditions

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global bioconjugation laboratory access with complete documentation packages supporting international standards and coupling method validations.

Research Grade Standards

High purity specifications for bioconjugation applications

ISO Standards

International standard compliance for research reagent specifications

Bioconjugation Grade

Bioconjugation grade specifications for protein studies

Global Compliance

International bioconjugation research regulation compliance

Method Validation

Supported coupling method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's bioconjugation team provides comprehensive method support, troubleshooting assistance, and coupling services to optimize EDC performance in your specific research applications.

Method Development
  • Bioconjugation optimization support
  • Coupling protocol guidance
  • Protein modification methods
  • Custom conjugation procedures
Research Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Coupling efficiency testing
  • Bioconjugation method validation support
Technical Support
  • Coupling troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our EDC production emphasizes environmental responsibility through sustainable synthesis practices, waste minimization, and comprehensive environmental impact management for bioconjugation research operations.

Waste Minimization

Optimized carbodiimide synthesis with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient organic synthesis with emission controls

Safe Disposal

Comprehensive guidance for organic waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art organic synthesis facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade EDC quality and performance across all production batches.

Production Process

Advanced organic synthesis and purification in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including NMR and HPLC analysis

Structural verification and purity analysis
Quality Systems

ISO 9001:2015 quality management with organic chemistry laboratory accreditation

Continuous improvement and method validation
Packaging Control

Moisture-resistant containers with desiccant protection

Hygroscopic stability protection and contamination prevention

Market Applications & Performance Data

Comprehensive bioconjugation research data demonstrating EDC effectiveness across diverse applications with quantified performance metrics and coupling method validations.

Research Laboratories
Purity: >98% carbodiimide content Efficiency: >85% coupling yield Stability: 24+ months at -20°C
Academic Institutions
Publication Support: 12000+ cited studies Reproducibility: >97% success rate Innovation: Custom conjugation solutions
Biotechnology Companies
Reliability: 99.7% batch consistency Quality Assurance: Validated coupling performance Efficiency: Optimized bioconjugation protocols

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds research specifications with ultra-high purity and exceptional coupling performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Bioconjugation Expertise

Dedicated bioconjugation team provides coupling optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed coupling performance data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services