T4 DNA Polymerase
Biotechnology Enzyme
Molecular Biology Enzymes

DNA Polymerases (T4 DNA Polymerase)

High-activity T4 DNA Polymerase engineered for molecular biology applications. Features excellent 3' to 5' exonuclease activity for DNA manipulation, cloning procedures, and research applications requiring precise DNA synthesis and editing capabilities.

  • High-Activity Enzyme
  • 3' to 5' Exonuclease Activity
  • DNA Manipulation
  • Cloning Applications
  • Research Grade Quality
  • Molecular Biology Validated
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Technical Specifications

Enzyme Name: T4 DNA Polymerase
Activity: 3,000 units/mL
5'-3' Polymerase: Yes
3'-5' Exonuclease: Yes (100x higher than Pol I)
5'-3' Exonuclease: No
Processivity: High (>1000 nucleotides)
Optimal Temperature: 37°C
Heat Inactivation: 75°C for 10 minutes
Buffer System: NEBuffer 2.1 or T4 DNA Polymerase buffer
Storage Buffer: 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 7.4
Storage Temperature: -20°C
Packaging Options: 150 units, 750 units, 3,000 units

Applications

3' Overhang Removal
Blunt-End Generation
DNA Sequencing
Site-Directed Mutagenesis
Molecular Cloning
Educational Research
DNA Fingerprinting
Probe Labeling
Plant Genomics
Second-Strand Synthesis
DNA Repair Studies
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized T4 DNA Polymerase grades tailored for specific molecular biology and cloning applications, ensuring optimal exonuclease activity and regulatory compliance across diverse research applications.

Research Grade
Activity: 3,000 U/mL Exonuclease: High Buffer: Included Application: General cloning
High-Activity Grade
Activity: 5,000 U/mL Processivity: Enhanced Stability: Extended Application: Demanding protocols
Ultra-Pure Grade
Activity: 3,000 U/mL Contamination: None Endotoxin: <0.1 EU/μL Application: Sensitive assays
Clinical Grade
Activity: 3,000 U/mL GMP: Compliant Validation: Clinical Application: Diagnostics

Quality Standards

DRAVYOM's T4 DNA Polymerase is manufactured under stringent quality control protocols, meeting international molecular biology standards including ISO, GMP, and research specifications. Our enzyme production ensures consistent activity and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Molecular Biology Grade
3,000 U/mL Activity Verified
Exonuclease Activity Testing
Processivity Assessment
Stability Testing
Certificate of Analysis
Cold Chain Logistics

Advanced Chemical Properties & Performance

T4 DNA Polymerase exhibits exceptional processivity and proofreading properties essential for DNA manipulation applications. Its 3'-5' exonuclease activity and high fidelity ensure reliable performance in demanding molecular cloning protocols and DNA synthesis studies.

Enzyme Properties
Enzyme Type: DNA polymerase (high-fidelity)
Source: Bacteriophage T4
Proofreading Activity: 3'-5' exonuclease
Processivity: Very high (>1000 nt)
Thermal Properties
Optimal Temperature: 37°C
Working Range: 25-42°C
Heat Inactivation: 65°C for 20 minutes
Thermal Stability: Mesophilic enzyme
Catalytic Performance
Extension Rate: 750 nt/min at 37°C
Fidelity: 100x higher than Taq
Error Rate: 1.7 × 10⁻⁶ errors/bp
Strand Displacement: Strong strand displacement
Purity Specifications
Enzyme Purity: ≥ 95% (SDS-PAGE)
Specific Activity: ≥ 5000 U/mg
Contaminating Activities: Not detected
Endotoxin: ≤ 0.1 EU/μL
Stability Properties
Shelf Life: 2 years (-20°C)
Working Solution: 1 month at 4°C
Freeze-Thaw Cycles: Up to 5 cycles
Activity Retention: ≥ 90% after 2 years

Performance Characteristics

Detailed performance metrics demonstrate T4 DNA Polymerase superiority in DNA manipulation applications with exceptional processivity, proofreading activity, and reproducibility across diverse molecular cloning protocols and DNA synthesis studies.

Fidelity Performance

Error rate: 1.7 × 10⁻⁶ errors/bp

100x higher fidelity than Taq
Processivity

Processivity: >1000 nucleotides

Very high processivity
Extension Rate

Rate: 750 nt/min at 37°C

Rapid extension kinetics
Strand Displacement

Displacement: Strong activity

Excellent strand displacement
Batch Reproducibility

Variation: ±5% between batches

Consistent enzyme performance
Stability Performance

2 years under proper storage

Extended enzyme integrity

Safety Information

Generally safe purified enzyme for research use. Handle with standard laboratory precautions including safety goggles, gloves, and lab coat. Non-toxic protein. Avoid contamination with nucleases. Use sterile techniques when handling solutions. Store in glycerol-containing buffer.

Generally Safe
Purified Enzyme
Standard Lab Precautions

Storage & Handling

Store in original containers at -20°C in glycerol-containing buffer. Keep containers tightly closed to prevent contamination. Avoid repeated freeze-thaw cycles. Use sterile techniques when preparing aliquots. Protect from nuclease contamination.

Frozen storage (-20°C)
Sterile technique required
Avoid repeated freeze-thaw
Glycerol-containing buffer

Chemical Mechanisms & Reaction Pathways

T4 DNA polymerase demonstrates exceptional versatility through dual polymerase and exonuclease activities, enabling precise molecular cloning applications with superior DNA manipulation capabilities and quantitative molecular biology performance for diverse research protocols.

DNA Polymerization

5' to 3' DNA synthesis with moderate processivity

Essential for molecular cloning
Exonuclease Activity

3' to 5' and 5' to 3' exonuclease activities for DNA processing

Critical for end processing
Fill-in Reactions

DNA end-filling and blunt-end generation capabilities

Enables cloning applications
Strand Displacement

Limited strand displacement synthesis activity

Supporting DNA manipulation

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global molecular biology laboratory access with complete documentation packages supporting international standards and cloning method validations.

Research Grade Standards

High activity specifications for molecular biology applications

ISO Standards

International standard compliance for research reagent specifications

Cloning Grade

Cloning grade specifications for molecular biology studies

Global Compliance

International molecular biology research regulation compliance

Method Validation

Supported cloning method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's molecular biology team provides comprehensive method support, troubleshooting assistance, and cloning services to optimize T4 DNA polymerase performance in your specific research applications.

Method Development
  • Cloning optimization support
  • DNA manipulation protocol guidance
  • End-processing analysis methods
  • Custom cloning procedures
Research Services
  • Certificate of Analysis verification
  • Custom activity analysis
  • Enzyme performance testing
  • Molecular biology method validation support
Technical Support
  • Cloning troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our T4 DNA polymerase production emphasizes environmental responsibility through sustainable enzyme preparation practices, waste minimization, and comprehensive environmental impact management for molecular biology research operations.

Waste Minimization

Optimized enzyme production with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient enzyme processing with emission controls

Safe Disposal

Comprehensive guidance for enzyme waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art enzyme manufacturing facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade T4 DNA polymerase quality and performance across all production batches.

Production Process

Advanced recombinant expression and purification in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including activity assays and exonuclease verification

Enzymatic activity verification and dual function testing
Quality Systems

ISO 9001:2015 quality management with molecular biology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Frozen storage containers with glycerol-stabilized formulation

Enzyme stability protection and contamination prevention

Market Applications & Performance Data

Comprehensive molecular biology research data demonstrating T4 DNA polymerase effectiveness across diverse applications with quantified performance metrics and cloning method validations.

Research Laboratories
Activity: >3000 units/mg protein Exonuclease: Dual 3'-5' and 5'-3' Stability: 12+ months at -20°C
Academic Institutions
Publication Support: 8000+ cited studies Reproducibility: >98% success rate Innovation: Custom cloning solutions
Biotechnology Companies
Reliability: 99.8% batch consistency Quality Assurance: Validated enzymatic activity Efficiency: Optimized cloning protocols

DRAVYOM Competitive Advantages

Superior Activity

Consistently exceeds research specifications with ultra-high activity and exceptional dual-function performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Molecular Biology Expertise

Dedicated molecular biology team provides cloning optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed enzymatic performance data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services