Phusion DNA Polymerase
Biotechnology High-Fidelity
High-Fidelity PCR Enzymes

DNA Polymerases (Phusion)

High-fidelity Phusion DNA Polymerase engineered for superior PCR performance. Combines exceptional accuracy with rapid extension speed and robust 3' to 5' exonuclease proofreading activity for demanding molecular biology applications.

  • High-Fidelity Performance
  • Superior Accuracy
  • Rapid Extension Speed
  • Proofreading Activity
  • PCR Optimization
  • Research Validated
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Technical Specifications

Enzyme Name: Phusion High-Fidelity DNA Polymerase
Activity: 2,000 units/mL
Error Rate: 4.4 × 10⁻⁷ (50x lower than Taq)
Extension Rate: 15-30 sec/kb at 72°C
Processivity: >300 nucleotides
Half-life: 7.5 min at 98°C
3'-5' Exonuclease: Yes (proofreading)
5'-3' Exonuclease: No
Storage Buffer: 20 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 7.4
Storage Temperature: -20°C
PCR Product: Blunt-ended
Packaging Options: 100 units, 500 units, 2,500 units

Applications

High-Fidelity PCR
Cloning Applications
Site-Directed Mutagenesis
Long-Range PCR
Primer Extension
Educational Research
Genomic Analysis
Gene Expression
Plant Genomics
Amplicon Sequencing
Library Preparation
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized Phusion DNA Polymerase grades tailored for specific PCR and molecular cloning applications, ensuring optimal fidelity and regulatory compliance across diverse research applications.

Research Grade
Activity: 2,000 U/mL Fidelity: High Buffer: Included Application: General PCR
Master Mix Grade
Format: Ready-to-use dNTPs: Pre-mixed MgCl2: Optimized Application: High-throughput
Ultra-Pure Grade
Activity: 5,000 U/mL Contamination: None Endotoxin: <0.1 EU/μL Application: Sensitive assays
Clinical Grade
Activity: 2,000 U/mL GMP: Compliant Validation: Clinical Application: Diagnostics

Quality Standards

DRAVYOM's Phusion DNA Polymerase is manufactured under stringent quality control protocols, meeting international molecular biology standards including ISO, GMP, and research specifications. Our enzyme production ensures consistent fidelity and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
High-Fidelity Specifications
2,000 U/mL Activity Verified
Fidelity Testing
Processivity Assessment
Thermostability Testing
Certificate of Analysis
Cold Chain Logistics

Advanced Chemical Properties & Performance

Phusion DNA Polymerase exhibits exceptional high-fidelity enzyme properties essential for PCR applications. Its proofreading activity and thermal stability ensure reliable performance in demanding amplification protocols and molecular cloning studies.

Enzyme Properties
Enzyme Type: DNA polymerase (high-fidelity)
Source: Pyrococcus furiosus
Proofreading Activity: 3'-5' exonuclease
Fidelity: 50x higher than Taq
Thermal Properties
Optimal Temperature: 72°C
Heat Activation: 98°C for 30 seconds
Half-Life: 5 minutes at 98°C
Thermal Stability: Extremely thermostable
Catalytic Performance
Extension Rate: 15-30 sec/kb
Processivity: High processivity (>1000 nt)
Error Rate: 4.4 × 10⁻⁷ errors/bp
GC-Rich Templates: Excellent performance
Purity Specifications
Enzyme Purity: ≥ 95% (SDS-PAGE)
Specific Activity: ≥ 1000 U/mg
Contaminating Activities: Not detected
Endotoxin: ≤ 0.1 EU/μL
Stability Properties
Shelf Life: 2 years (-20°C)
Working Solution: 1 month at 4°C
Freeze-Thaw Cycles: Up to 5 cycles
Activity Retention: ≥ 90% after 2 years

Performance Characteristics

Detailed performance metrics demonstrate Phusion DNA Polymerase superiority in PCR applications with exceptional fidelity, thermal stability, and reproducibility across diverse amplification protocols and molecular cloning studies.

Fidelity Performance

Error rate: 4.4 × 10⁻⁷ errors/bp

50x higher fidelity than Taq
Thermal Stability

Half-life: 5 min at 98°C

Extremely thermostable
Extension Rate

Rate: 15-30 sec/kb

Fast extension kinetics
GC-Rich Performance

GC content: Up to 80%

Excellent difficult template amplification
Batch Reproducibility

Variation: ±5% between batches

Consistent enzyme performance
Stability Performance

2 years under proper storage

Extended enzyme integrity

Safety Information

Generally safe purified enzyme for research use. Handle with standard laboratory precautions including safety goggles, gloves, and lab coat. Non-toxic protein. Avoid contamination with nucleases. Use sterile techniques when handling solutions. Store in glycerol-containing buffer.

Generally Safe
Purified Enzyme
Standard Lab Precautions

Storage & Handling

Store in original containers at -20°C in glycerol-containing buffer. Keep containers tightly closed to prevent contamination. Avoid repeated freeze-thaw cycles. Use sterile techniques when preparing aliquots. Protect from nuclease contamination.

Frozen storage (-20°C)
Sterile technique required
Avoid repeated freeze-thaw
Glycerol-containing buffer

Chemical Mechanisms & Reaction Pathways

Phusion DNA polymerase demonstrates exceptional fidelity through 3' to 5' exonuclease proofreading activity, enabling precise PCR amplification applications with superior accuracy and quantitative molecular biology performance for demanding research protocols.

DNA Polymerization

5' to 3' DNA synthesis with exceptional processivity and speed

Essential for high-fidelity PCR
Proofreading Activity

3' to 5' exonuclease activity for error correction

Critical for accuracy enhancement
Thermal Stability

Heat-stable enzyme maintains activity at high temperatures

Enables PCR cycling
Processivity Enhancement

Extended DNA synthesis without dissociation

Supporting long amplicon generation

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global molecular biology laboratory access with complete documentation packages supporting international standards and PCR method validations.

Research Grade Standards

High activity specifications for molecular biology applications

ISO Standards

International standard compliance for research reagent specifications

PCR Grade

PCR grade specifications for amplification studies

Global Compliance

International molecular biology research regulation compliance

Method Validation

Supported PCR method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's molecular biology team provides comprehensive method support, troubleshooting assistance, and PCR services to optimize Phusion DNA polymerase performance in your specific research applications.

Method Development
  • PCR optimization support
  • Amplification protocol guidance
  • High-fidelity analysis methods
  • Custom PCR procedures
Research Services
  • Certificate of Analysis verification
  • Custom activity analysis
  • Enzyme performance testing
  • Molecular biology method validation support
Technical Support
  • PCR troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our Phusion DNA polymerase production emphasizes environmental responsibility through sustainable enzyme preparation practices, waste minimization, and comprehensive environmental impact management for molecular biology research operations.

Waste Minimization

Optimized enzyme production with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient enzyme processing with emission controls

Safe Disposal

Comprehensive guidance for enzyme waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art enzyme manufacturing facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade Phusion DNA polymerase quality and performance across all production batches.

Production Process

Advanced recombinant expression and purification in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including activity assays and fidelity verification

Enzymatic activity verification and performance testing
Quality Systems

ISO 9001:2015 quality management with molecular biology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Frozen storage containers with glycerol-stabilized formulation

Enzyme stability protection and contamination prevention

Market Applications & Performance Data

Comprehensive molecular biology research data demonstrating Phusion DNA polymerase effectiveness across diverse applications with quantified performance metrics and PCR method validations.

Research Laboratories
Fidelity: 50x higher than Taq Speed: 15-30 sec/kb extension Stability: 12+ months at -20°C
Academic Institutions
Publication Support: 15000+ cited studies Reproducibility: >99% success rate Innovation: Custom PCR solutions
Biotechnology Companies
Reliability: 99.9% batch consistency Quality Assurance: Validated enzymatic activity Efficiency: Optimized amplification protocols

DRAVYOM Competitive Advantages

Superior Activity

Consistently exceeds research specifications with ultra-high activity and exceptional fidelity performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Molecular Biology Expertise

Dedicated molecular biology team provides PCR optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed enzymatic performance data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services