DNA Ladder Molecular Weight Marker
Biotechnology Size Marker
Molecular Biology Standards

DNA Ladder (Molecular Weight Marker)

High-quality DNA ladder molecular weight marker essential for electrophoresis applications. Provides accurate size standards for precise DNA fragment size determination in gel analysis and molecular biology procedures.

  • High-Quality Size Standard
  • Accurate Size Determination
  • Electrophoresis Compatible
  • Multiple Size Ranges
  • Molecular Biology Grade
  • Research Validated

Technical Specifications

Product Type: DNA Molecular Weight Ladder
Fragment Range: 100 bp - 10,000 bp
Fragment Sizes: 100, 200, 300, 400, 500, 750, 1000, 1500, 2000, 3000, 5000, 10000 bp
Concentration: 0.1 μg/μL
Loading Buffer: 6X included
Volume per Lane: 5-10 μL recommended
Storage Buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0
Storage Temperature: -20°C
Stability: 2 years at -20°C
Gel Types: Agarose, Polyacrylamide
Ready-to-Use: Yes (with loading dye)
Packaging Options: 50 lanes, 100 lanes, 250 lanes

Applications

DNA Size Determination
Gel Electrophoresis
PCR Product Analysis
Restriction Analysis
Molecular Cloning
Educational Research
Quality Control
Fragment Quantification
Plant Genomics
Southern Blotting
Forensic Analysis
Reference Standard

Industry-Specific Grades

DRAVYOM offers specialized DNA ladder grades tailored for specific molecular biology and analytical applications, ensuring accurate size determination and regulatory compliance across diverse research applications.

Research Grade
Range: 100 bp - 10 kb Accuracy: ±5% Bands: 12 fragments Application: General research
High-Resolution Grade
Range: 50 bp - 1 kb Accuracy: ±2% Bands: 20 fragments Application: Precise sizing
Wide-Range Grade
Range: 100 bp - 50 kb Accuracy: ±3% Bands: 15 fragments Application: Large fragments
Forensic Grade
Range: 75 bp - 20 kb Accuracy: ±1% Validation: Forensic Application: Legal analysis

Quality Standards

DRAVYOM's DNA Ladder is manufactured under stringent quality control protocols, meeting international molecular biology standards including research specifications and validation requirements. Our ladder production ensures consistent sizing accuracy and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Molecular Biology Grade
Sizing Accuracy Verified
Gel Migration Testing
Fragment Integrity Assessment
Stability Testing
Certificate of Analysis
Cold Chain Logistics

Advanced Chemical Properties & Performance

DNA Ladder exhibits exceptional molecular weight marker properties essential for electrophoresis applications. Its precisely sized DNA fragments and size reference standards ensure reliable performance in demanding gel electrophoresis protocols and molecular weight determination studies.

Molecular Marker Properties
Fragment Range: 100-10,000 bp
Fragment Count: 12-15 discrete bands
Reference Standards: Logarithmic size distribution
Band Intensity: Uniform ethidium bromide staining
Physical Properties
DNA Source: Lambda phage DNA digest
Restriction Enzyme: HindIII or EcoRI
Buffer System: TE buffer (pH 8.0)
Loading Concentration: 0.5-1.0 μg/lane
Electrophoresis Performance
Gel Compatibility: Agarose, polyacrylamide
Running Buffer: TAE, TBE compatible
Migration Pattern: Linear with log molecular weight
Resolution: Sharp, well-defined bands
Purity Specifications
DNA Purity: ≥ 95% (A260/A280 ≥ 1.8)
Fragment Accuracy: ±5% of stated size
Nuclease Activity: Not detected
Bacterial Contamination: Not detected
Stability Properties
Shelf Life: 2 years (-20°C)
Working Solution: 6 months at 4°C
Freeze-Thaw Cycles: Up to 10 cycles
DNA Integrity: Maintained under proper storage

Performance Characteristics

Detailed performance metrics demonstrate DNA Ladder superiority in molecular weight determination with exceptional band resolution, sizing accuracy, and reproducibility across diverse gel electrophoresis protocols and fragment analysis studies.

Sizing Accuracy

Fragment size: ±5% of stated value

Precise molecular weight reference
Band Resolution

Resolution: Sharp, well-defined bands

Excellent electrophoretic mobility
Staining Intensity

Visibility: Uniform ethidium bromide staining

Consistent band intensity
Temperature Stability

Working range: 4-65°C

Stable across electrophoresis conditions
Batch Reproducibility

Variation: ±2% between batches

Consistent marker performance
Stability Performance

2 years under proper storage

Extended DNA integrity

Safety Information

Generally safe purified DNA for research use. Handle with standard laboratory precautions including safety goggles, gloves, and lab coat. Non-toxic and biodegradable. Avoid contamination with nucleases. Use sterile techniques when handling solutions.

Generally Safe
Biodegradable
Standard Lab Precautions

Storage & Handling

Store in original containers at -20°C in a dry environment. Keep containers tightly closed to prevent contamination. Avoid repeated freeze-thaw cycles. Use sterile techniques when preparing aliquots. Protect from nuclease contamination.

Frozen storage (-20°C)
Sterile technique required
Avoid repeated freeze-thaw
Prevent nuclease contamination

Chemical Mechanisms & Reaction Pathways

DNA ladder provides size standards through precisely defined fragment lengths, enabling accurate molecular weight determination applications with predictable migration patterns and quantitative gel electrophoresis for molecular biology studies.

Electrophoretic Migration

Size-dependent DNA fragment migration in agarose gels

Essential for molecular weight determination
Fragment Sizing

Logarithmic relationship between fragment size and migration distance

Critical for accurate size determination
DNA Visualization

Ethidium bromide or SYBR staining for fluorescent detection

Enables gel documentation
Buffer Compatibility

Stable performance across various electrophoresis buffer systems

Supporting diverse gel protocols

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global molecular biology laboratory access with complete documentation packages supporting international standards and DNA analysis method validations.

Research Grade Standards

High purity specifications for molecular biology applications

ISO Standards

International standard compliance for research reagent specifications

Molecular Weight Standard Grade

Molecular weight standard specifications for DNA studies

Global Compliance

International molecular biology research regulation compliance

Method Validation

Supported gel electrophoresis method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's molecular biology team provides comprehensive method support, troubleshooting assistance, and electrophoresis services to optimize DNA ladder performance in your specific research applications.

Method Development
  • Gel electrophoresis optimization support
  • DNA sizing protocol guidance
  • Molecular weight methods
  • Custom electrophoresis procedures
Research Services
  • Certificate of Analysis verification
  • Custom fragment sizing
  • Migration pattern testing
  • Molecular biology method validation support
Technical Support
  • Gel troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our DNA ladder production emphasizes environmental responsibility through sustainable DNA preparation practices, waste minimization, and comprehensive environmental impact management for molecular biology research operations.

Waste Minimization

Optimized DNA preparation with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient DNA processing with emission controls

Safe Disposal

Comprehensive guidance for DNA waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art molecular biology manufacturing facility employs advanced DNA preparation technology and continuous monitoring systems to ensure consistent research-grade DNA ladder quality and performance across all production batches.

Production Process

Advanced DNA fragment preparation and verification in controlled environment

Multi-stage verification for research grade quality
Quality Testing

Comprehensive analytical testing including gel electrophoresis and fragment analysis

Migration pattern verification and size confirmation
Quality Systems

ISO 9001:2015 quality management with molecular biology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Frozen storage containers with nuclease-free conditions

DNA integrity protection and contamination prevention

Market Applications & Performance Data

Comprehensive molecular biology research data demonstrating DNA ladder effectiveness across diverse applications with quantified performance metrics and gel electrophoresis method validations.

Research Laboratories
Purity: >95% DNA content Accuracy: ±5% size determination Stability: 24+ months at -20°C
Academic Institutions
Publication Support: 8000+ cited studies Reproducibility: >99% inter-lab agreement Innovation: Custom size standard solutions
Biotechnology Companies
Reliability: 99.7% batch consistency Quality Assurance: Validated migration patterns Efficiency: Optimized gel protocols

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds research specifications with ultra-high purity and exceptional sizing performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Molecular Biology Expertise

Dedicated molecular biology team provides gel optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed sizing performance data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services