Disodium Hydrogen Phosphate
Biotechnology Buffer Component
Buffer Components

Disodium Hydrogen Phosphate

High-purity disodium hydrogen phosphate essential for biotechnology applications. Provides reliable phosphate buffering for cell culture media, biochemical procedures, and molecular biology techniques requiring precise pH control.

  • High-Purity Buffer Component
  • Phosphate Buffering
  • Cell Culture Compatible
  • pH Control
  • Biochemical Research Grade
  • Laboratory Validated
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Technical Specifications

Chemical Name: Disodium hydrogen phosphate
CAS Number: 7558-79-4
Molecular Formula: Na2HPO4
Molecular Weight: 141.96 g/mol (anhydrous)
Physical State: White crystalline powder
Solubility: Water (119 g/100mL at 25°C)
pH (1% solution): 8.5-9.5
Buffer Range: pH 6.0-8.0 (with NaH2PO4)
Storage Temperature: Room temperature (Dry place)
Purity: ≥99% (ACS Grade)
Water Content: ≤0.5% (Karl Fischer)
Packaging Options: 100g, 500g, 1kg containers

Applications

PBS Buffer Systems
Cell Culture Media
Biochemical Research
Molecular Biology
pH Control Systems
Educational Research
Protein Studies
DNA/RNA Work
Plant Biology
Enzyme Assays
Immunoassays
Quality Control

Industry-Specific Grades

DRAVYOM offers specialized disodium hydrogen phosphate grades tailored for specific buffer and cell culture applications, ensuring optimal pH stability and regulatory compliance across diverse biotechnology applications.

Research Grade
Purity: ≥99% (ACS) Heavy Metals: <10 ppm Water Content: ≤0.5% Application: General research
PBS Grade
Purity: ≥99.5% (ACS) Endotoxin: <1 EU/g pH Buffer: Optimized Application: PBS preparation
Cell Culture Grade
Purity: ≥99.9% (USP) Sterility: Tested Cytotoxicity: None Application: Cell culture
Pharmaceutical Grade
Purity: ≥99.9% (USP/EP) GMP: Compliant Microbial: Tested Application: Drug formulation

Quality Standards

DRAVYOM's Disodium Hydrogen Phosphate is manufactured under stringent quality control protocols, meeting international buffer standards including ACS, USP, and research specifications. Our buffer production ensures consistent pH stability and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
ACS Grade Specifications
≥99% ACS Verified Purity
pH Buffer Testing
Buffer Capacity Assessment
Endotoxin Testing
Certificate of Analysis
Moisture-Protected Packaging

Advanced Chemical Properties & Performance

Disodium Hydrogen Phosphate exhibits exceptional buffering salt properties essential for biochemical applications. Its phosphate buffering capacity and pH stabilization ensure reliable performance in demanding buffer system protocols and enzymatic studies.

Buffer Properties
Molecular Weight: 141.96 Da (anhydrous)
Phosphate Form: Dibasic phosphate (HPO₄²⁻)
Buffer Range: pH 6.8-8.0
pKa Values: 7.21 (25°C)
Physical Properties
Appearance: White crystalline powder
Solubility (25°C): 1190 g/L in water
Crystal Structure: Orthorhombic system
Hygroscopicity: Slightly hygroscopic
Biochemical Performance
Buffer Capacity: Excellent near pH 7.2
Ionic Strength: Higher than potassium salt
Enzyme Compatibility: Compatible with most enzymes
Metal Chelation: Minimal metal binding
Purity Specifications
Assay (dry basis): ≥ 99%
Heavy Metals: ≤ 10 ppm
Chloride: ≤ 0.005%
Insoluble Matter: ≤ 0.01%
Stability Properties
Shelf Life: 5 years (room temperature)
pH Stability: Excellent buffer stability
Solution Stability: Indefinite at room temperature
Temperature Stability: Stable up to 100°C

Performance Characteristics

Detailed performance metrics demonstrate Disodium Hydrogen Phosphate superiority in buffer applications with exceptional pH stability, buffering capacity, and reproducibility across diverse biochemical protocols and enzymatic studies.

Buffer Capacity

pH buffering: Excellent near 7.2

Strong phosphate buffering
Solubility Performance

Water solubility: 1190 g/L at 25°C

Excellent aqueous dissolution
Enzyme Compatibility

Enzyme activity: No interference

Compatible with most enzymes
Temperature Stability

Working range: 4-100°C

Stable across wide temperature range
Batch Reproducibility

Variation: ±0.1% between batches

Consistent buffer performance
Stability Performance

5 years under proper storage

Extended buffer integrity

Safety Information

Generally safe inorganic salt for research use. Handle with standard laboratory precautions including safety goggles, gloves, and lab coat. May cause mild irritation to eyes and respiratory tract. Avoid inhalation of dust and direct contact with eyes. Non-toxic at normal usage levels.

Generally Safe
Mild Eye Irritant
Standard Lab Precautions

Storage & Handling

Store in original containers at room temperature in a dry environment. Keep containers tightly closed to prevent moisture absorption. Solutions can be sterilized and stored at room temperature for extended periods. Stable under normal laboratory conditions.

Room temperature storage
Dry environment required
Original containers tightly closed
Stable under normal conditions

Chemical Mechanisms & Reaction Pathways

Disodium hydrogen phosphate serves as a pH buffer through phosphate equilibrium systems, enabling precise biochemical research applications with predictable buffering capacity and quantitative pH control for biological studies.

Buffer Equilibrium

HPO₄²⁻/H₂PO₄⁻ equilibrium system for pH buffering

Essential for biological pH control
Ionic Dissociation

Complete dissociation to provide sodium and phosphate ions

Critical for buffer capacity
Enzyme Stabilization

Phosphate buffer system for protein and enzyme stability

Enables biochemical assays
Osmotic Regulation

Osmotic pressure control in biological systems

Supporting cell culture applications

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global biochemistry laboratory access with complete documentation packages supporting international standards and buffer system method validations.

Research Grade Standards

High purity specifications for biochemistry applications

ISO Standards

International standard compliance for research reagent specifications

Buffer Grade

Buffer grade specifications for biological studies

Global Compliance

International biochemistry research regulation compliance

Method Validation

Supported buffer method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's biochemistry team provides comprehensive method support, troubleshooting assistance, and buffer services to optimize disodium hydrogen phosphate performance in your specific research applications.

Method Development
  • Buffer preparation optimization support
  • pH calibration protocol guidance
  • Biochemical assay methods
  • Custom buffer procedures
Research Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Buffer capacity testing
  • Biochemistry method validation support
Technical Support
  • Buffer troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our disodium hydrogen phosphate production emphasizes environmental responsibility through sustainable synthesis practices, waste minimization, and comprehensive environmental impact management for biochemistry research operations.

Waste Minimization

Optimized phosphate synthesis with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient phosphate synthesis with emission controls

Safe Disposal

Comprehensive guidance for phosphate waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art inorganic manufacturing facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade disodium hydrogen phosphate quality and performance across all production batches.

Production Process

Advanced phosphate synthesis and crystallization in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including ion chromatography and pH analysis

Ionic composition verification and buffer capacity testing
Quality Systems

ISO 9001:2015 quality management with biochemistry laboratory accreditation

Continuous improvement and method validation
Packaging Control

Moisture-resistant containers with sealed packaging

Hygroscopic protection and contamination prevention

Market Applications & Performance Data

Comprehensive biochemistry research data demonstrating disodium hydrogen phosphate effectiveness across diverse applications with quantified performance metrics and buffer system method validations.

Research Laboratories
Purity: >99% phosphate content Buffer Capacity: pH 6.8-8.0 range Stability: 36+ months ambient storage
Academic Institutions
Publication Support: 2500+ cited protocols Reproducibility: >99% inter-lab agreement Innovation: Custom buffer solutions
Biotechnology Companies
Reliability: 99.8% batch consistency Quality Assurance: Validated buffer performance Efficiency: Optimized biochemical protocols

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds research specifications with ultra-high purity and exceptional buffer performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Biochemistry Expertise

Dedicated biochemistry team provides buffer optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed buffer performance data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services