DAPI Fluorescent Nuclear Stain
High-quality DAPI (4',6-diamidino-2-phenylindole) for fluorescence microscopy and nuclear staining. Premium grade fluorescent dye manufactured for DNA visualization and cell counting applications.
- Nuclear DNA Binding
- UV Fluorescence Excitation
- High Specificity for DNA
- Cell Counting Compatible
- Microscopy Grade Purity
- Research Grade Quality
Technical Specifications
Applications
Industry-Specific Grades
DRAVYOM offers specialized DAPI grades tailored for specific research and diagnostic applications, ensuring optimal fluorescence performance and regulatory compliance across diverse biotechnology applications.
Research Grade
Microscopy Grade
Flow Cytometry Grade
Clinical Grade
Quality Standards
DRAVYOM's DAPI is manufactured under stringent quality control protocols, meeting international biotechnology standards including ISO, cGMP, and research specifications. Our analytical-grade production ensures consistent fluorescence performance and regulatory compliance.
Advanced Chemical Properties & Performance
DAPI exhibits exceptional fluorescent dye properties essential for nucleic acid visualization applications. Its DNA-binding specificity and fluorescence enhancement ensure reliable performance in demanding microscopy protocols and cell nuclear staining studies.
Fluorescent Properties
Physical Properties
DNA Binding Performance
Purity Specifications
Stability Properties
Performance Characteristics
Detailed performance metrics demonstrate DAPI superiority in fluorescence microscopy applications with exceptional DNA binding specificity, fluorescence enhancement, and reproducibility across diverse nuclear staining protocols and cell imaging studies.
Fluorescence Enhancement
Enhancement: 20-fold upon DNA binding
Strong fluorescence signalDNA Binding Specificity
Minor groove binding: AT-rich regions
Selective nucleic acid interactionNuclear Staining
Staining concentration: 0.1-1 μg/mL
Excellent nuclear visualizationTemperature Stability
Working range: 4-37°C
Stable across biological temperaturesBatch Reproducibility
Fluorescence variation: ±5%
Consistent staining performanceStability Performance
3 years under proper storage
Extended fluorophore integritySafety Information
Potential mutagen requiring special handling precautions. Handle with standard laboratory safety equipment including safety goggles, gloves, and lab coat. Avoid inhalation of powder and skin contact. DNA-binding agent - handle in well-ventilated areas. Dispose according to hazardous waste regulations.
Storage & Handling
Store in original containers at -20°C in a dark, dry environment. Keep containers tightly closed with desiccant. Protect from light during storage and use. Prepare working solutions fresh and use immediately for optimal fluorescence.
Chemical Mechanisms & Reaction Pathways
DAPI binds to DNA through AT-rich region intercalation, enabling precise nuclear imaging applications with predictable fluorescence enhancement and quantitative nucleic acid detection for cell biology studies.
DNA Intercalation
Selective binding to AT-rich regions of double-stranded DNA
Essential for nuclear stainingFluorescence Enhancement
20-fold fluorescence increase upon DNA binding
Critical for live cell imagingSpectral Properties
Blue fluorescence with excitation at 358nm, emission at 461nm
Enables fluorescence microscopyCell Permeability
Membrane-permeable staining for live and fixed cells
Supporting cell viability studiesRegulatory Compliance & Documentation
Comprehensive regulatory compliance ensures global cell biology laboratory access with complete documentation packages supporting international standards and fluorescence imaging method validations.
Research Grade Standards
High purity specifications for cell biology applications
ISO Standards
International standard compliance for research reagent specifications
Fluorescence Grade
Fluorescence grade specifications for imaging studies
Global Compliance
International cell biology research regulation compliance
Method Validation
Supported fluorescence method validation documentation
SDS Documentation
Multi-language Safety Data Sheets (16 sections, GHS compliant)
Technical Support & Value-Added Services
DRAVYOM's cell biology team provides comprehensive method support, troubleshooting assistance, and fluorescence services to optimize DAPI performance in your specific research applications.
Method Development
- Fluorescence microscopy optimization support
- Cell staining protocol guidance
- Imaging parameter development
- Custom fluorescence procedures
Research Services
- Certificate of Analysis verification
- Custom purity analysis
- Fluorescence quality testing
- Cell biology method validation support
Technical Support
- Fluorescence troubleshooting consultation
- Storage and handling guidance
- Laboratory safety protocols
- Research best practices
Supply Solutions
- Consistent batch scheduling
- Emergency supply arrangements
- Custom packaging options
- Global distribution network
Environmental Impact & Sustainability
Our DAPI production emphasizes environmental responsibility through sustainable fluorophore synthesis practices, waste minimization, and comprehensive environmental impact management for cell biology research operations.
Waste Minimization
Optimized fluorophore synthesis with minimal waste generation
Water Treatment
Advanced wastewater treatment and recycling systems
Green Production
Energy-efficient fluorophore synthesis with emission controls
Safe Disposal
Comprehensive guidance for fluorophore waste management
ISO 14001
Environmental management system certified production
Container Recycling
Sustainable container return and recycling programs
Manufacturing Excellence & Quality Control
DRAVYOM's state-of-the-art fluorophore manufacturing facility employs advanced synthesis technology and continuous monitoring systems to ensure consistent research-grade DAPI quality and performance across all production batches.
Production Process
Advanced fluorophore synthesis and purification in controlled environment
Multi-stage purification for research grade qualityQuality Testing
Comprehensive analytical testing including fluorescence and DNA binding analysis
Spectrofluorometric verification and binding assaysQuality Systems
ISO 9001:2015 quality management with cell biology laboratory accreditation
Continuous improvement and method validationPackaging Control
Light-protected containers with moisture and air exclusion
Fluorophore stability protection and contamination preventionMarket Applications & Performance Data
Comprehensive cell biology research data demonstrating DAPI effectiveness across diverse applications with quantified performance metrics and fluorescence imaging method validations.
Research Laboratories
Academic Institutions
Biotechnology Companies
DRAVYOM Competitive Advantages
Superior Purity
Consistently exceeds research specifications with ultra-high purity and exceptional fluorescence performance
Reliable Supply
Guaranteed availability with strategic inventory management and research-grade production scheduling
Cell Biology Expertise
Dedicated cell biology team provides fluorescence optimization and troubleshooting support
Quality Assurance
Comprehensive certificates with detailed fluorescence performance data and method validation support
Global Standards
International compliance with research specifications for worldwide acceptance
Partnership Approach
Collaborative relationships with research laboratories and custom grade development services