Crystal Violet Stain
Biotechnology Primary Stain
Bacterial Stains

Crystal Violet (Stain)

High-quality Crystal Violet essential for microbiology applications. Serves as the primary stain in Gram staining procedures for bacterial identification and classification, providing clear differential staining results.

  • High-Quality Primary Stain
  • Gram Staining Protocol
  • Bacterial Identification
  • Clear Differential Results
  • Microbiology Grade
  • Research Validated
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Technical Specifications

Chemical Name: Crystal Violet (Methyl Violet 10B)
Molecular Formula: C₂₅H₃₀ClN₃
CAS Number: 548-62-9
Molecular Weight: 407.98 g/mol
Purity: ≥90.0% (Microbiological Grade)
Physical Form: Dark green crystalline powder
λmax (Absorption): 590 nm
Solubility: Water, ethanol, methanol
Working Concentration: 0.5-1.0% aqueous solution
pH (1% solution): 6.0-7.0
Storage Temperature: Room temperature
Packaging Options: 5g, 25g, 100g bottles
Physical State: Clear, colorless liquid
Density (20°C): 1.18 g/cm³
Molecular Weight: 36.46 g/mol
Boiling Point: 108.6°C (azeotrope)
pH (1% solution): ≤ 1.0
Residue on Evaporation: ≤ 0.0005%
Free Chlorine (Cl): ≤ 0.0001%
Iron (Fe): ≤ 0.00001%
Heavy Metals (as Pb): ≤ 0.000001%
Sulfate (SO₄): ≤ 0.0005%
Nitrate (NO₃): ≤ 0.0001%
Arsenic (As): ≤ 0.000001%
UN Number: UN 1789 (Hydrochloric acid)
Packaging Options: 500ml, 1L, 2.5L amber glass bottles

Applications

Analytical Titrations
Sample Preparation & Digestion
Quantitative Analysis
Elemental Analysis (ICP-MS/AAS)
Research & Development
University Laboratories
Quality Control Testing
Pharmaceutical Analysis
Environmental Testing
Food Analysis
Mineral Analysis
Reference Standards

Industry-Specific Grades

DRAVYOM offers specialized hydrochloric acid grades tailored for specific analytical requirements, ensuring optimal performance and regulatory compliance across diverse laboratory applications.

AR Grade (Analytical Reagent)
Assay: 35-37% Residue: ≤ 0.0005% Heavy Metals: ≤ 0.000001% Application: Analytical procedures, titrations
ACS Grade (Certified)
Assay: 36.5-38.0% Iron: ≤ 0.00001% Sulfate: ≤ 0.0005% Application: ACS certified analytical work
Trace Metal Grade
Assay: 34-37% Metal Impurities: ≤ 0.1 ppm each Total Metals: ≤ 1 ppm Application: ICP-MS, AAS analysis
Ultra-Pure Grade
Assay: 36-38% Particle Count: ≤ 50 per mL Organics: ≤ 1 ppm Application: Semiconductor, electronics

Quality Standards

DRAVYOM's AR Grade Hydrochloric Acid is manufactured under stringent quality control protocols, meeting international analytical standards including ACS, ISO, and pharmacopoeia specifications. Our analytical-grade production ensures consistent performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
ACS Grade Specifications
35-37% Accurate Concentration
Advanced Analytical Testing
Ultra-Low Metal Impurities
Batch-to-Batch Consistency
Traceable Certificate of Analysis
Temperature-Controlled Logistics

Advanced Chemical Properties & Performance

Crystal Violet exhibits exceptional cationic dye properties essential for microbiology applications. Its gram-positive bacteria selectivity and staining capabilities ensure reliable performance in demanding bacteriological protocols and cell wall differentiation studies.

Dye Properties
Molecular Weight: 407.98 Da
Dye Class: Triphenylmethane
Charge: Cationic dye
Selectivity: Gram-positive bacteria
Physical Properties
Appearance: Dark violet crystals
Solubility: Water, ethanol soluble
Absorption Maximum: 590 nm
Extinction Coefficient: 87,000 M⁻¹cm⁻¹
Staining Performance
Gram Staining: Primary stain
Cell Wall Binding: Peptidoglycan interaction
Retention: Gram-positive retained
Contrast: High visibility staining
Purity Specifications
Dye Content: ≥ 90%
Staining Efficiency: Consistent gram differentiation
Heavy Metals: ≤ 50 ppm
Water Content: ≤ 3%
Stability Properties
Shelf Life: 5 years (room temperature)
Solution Stability: Months at room temperature
Light Sensitivity: Moderate photostability
pH Stability: Stable pH 6-8

Performance Characteristics

Detailed performance metrics demonstrate Crystal Violet superiority in microbiology applications with exceptional gram staining differentiation, bacterial selectivity, and reproducibility across diverse bacteriological protocols and cell identification studies.

Gram Differentiation

Selectivity: >95% gram-positive retention

Excellent bacterial differentiation
Cell Wall Binding

Peptidoglycan interaction: High affinity

Specific cell wall targeting
Visual Contrast

Staining intensity: High violet color

Excellent microscopic visibility
Temperature Stability

Working range: 15-37°C

Stable across working temperatures
Batch Reproducibility

Staining variation: ±3%

Consistent dye performance
Stability Performance

5 years under proper storage

Extended dye integrity

Safety Information

Potential carcinogen requiring special handling precautions. Handle with standard laboratory safety equipment including safety goggles, gloves, and lab coat. Avoid inhalation of powder and skin contact. Use in well-ventilated areas. Dispose according to hazardous waste regulations.

Potential Carcinogen
Avoid Skin Contact
Use with Ventilation

Storage & Handling

Store in original containers at room temperature in a dry environment. Keep containers tightly closed to prevent moisture absorption. Solutions can be stored at room temperature for extended periods. Handle with appropriate personal protective equipment.

Room temperature storage
Dry environment required
PPE required for handling
Hazardous waste disposal

Chemical Mechanisms & Reaction Pathways

Crystal violet binds to negatively charged cell components through electrostatic interactions, enabling precise bacterial identification applications with predictable staining patterns and quantitative cell wall analysis for microbiology studies.

Cell Wall Binding

Electrostatic binding to peptidoglycan and teichoic acids

Essential for Gram staining
Iodine Complex

Crystal violet-iodine complex formation and retention

Critical for bacterial differentiation
Chromophore System

Triphenylmethane dye structure for visible detection

Enables microscopic visualization
Decolorization Resistance

Differential alcohol decolorization in Gram-positive bacteria

Supporting bacterial classification

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global microbiology laboratory access with complete documentation packages supporting international standards and bacterial staining method validations.

Research Grade Standards

High purity specifications for microbiology applications

ISO Standards

International standard compliance for research reagent specifications

Microbiology Grade

Microbiology grade specifications for bacterial studies

Global Compliance

International microbiology research regulation compliance

Method Validation

Supported staining method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's microbiology team provides comprehensive method support, troubleshooting assistance, and staining services to optimize crystal violet performance in your specific research applications.

Method Development
  • Gram staining optimization support
  • Bacterial identification guidance
  • Staining protocol development
  • Custom microbiology procedures
Research Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Staining quality testing
  • Microbiology method validation support
Technical Support
  • Staining troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our crystal violet production emphasizes environmental responsibility through sustainable dye synthesis practices, waste minimization, and comprehensive environmental impact management for microbiology research operations.

Waste Minimization

Optimized dye synthesis with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient dye synthesis with emission controls

Safe Disposal

Comprehensive guidance for dye waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art dye manufacturing facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade crystal violet quality and performance across all production batches.

Production Process

Advanced dye synthesis and purification in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including spectroscopic and staining analysis

UV-Vis verification and staining performance testing
Quality Systems

ISO 9001:2015 quality management with microbiology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Dark containers with moisture protection

Dye stability protection and contamination prevention

Market Applications & Performance Data

Comprehensive microbiology research data demonstrating crystal violet effectiveness across diverse applications with quantified performance metrics and bacterial staining method validations.

Research Laboratories
Purity: >99% dye content Performance: Clear Gram differentiation Stability: 36+ months ambient storage
Academic Institutions
Publication Support: 3000+ cited protocols Reproducibility: >99% inter-lab agreement Innovation: Custom staining solutions
Clinical Laboratories
Reliability: 99.9% batch consistency Quality Assurance: Validated staining performance Efficiency: Optimized identification protocols

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds research specifications with ultra-high purity and exceptional staining performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Microbiology Expertise

Dedicated microbiology team provides staining optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed staining performance data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services