Coomassie Brilliant Blue Protein Stain
Biotechnology Protein Stain
Protein Analysis

Coomassie Brilliant Blue (Protein Stain)

High-quality Coomassie Brilliant Blue protein stain essential for electrophoresis applications. Provides excellent protein visualization in SDS-PAGE gels with high sensitivity and clear band definition for protein analysis.

  • High-Quality Protein Stain
  • Excellent Protein Visualization
  • SDS-PAGE Compatible
  • High Sensitivity
  • Clear Band Definition
  • Research Validated
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Technical Specifications

Chemical Name: Coomassie Brilliant Blue R-250
Molecular Formula: C₄₅H₄₄N₃NaO₇S₂
CAS Number: 6104-58-1
Molecular Weight: 825.97 g/mol
Purity: ≥90.0% (Electrophoresis Grade)
Physical Form: Blue metallic powder
λmax (Absorption): 555 nm
Solubility: Methanol, ethanol, water (limited)
Working Concentration: 0.1-0.25% in staining solution
Detection Limit: 0.1-1.0 μg protein
Storage Temperature: Room temperature
Packaging Options: 1g, 5g, 25g bottles
Physical State: Clear, colorless liquid
Density (20°C): 1.18 g/cm³
Molecular Weight: 36.46 g/mol
Boiling Point: 108.6°C (azeotrope)
pH (1% solution): ≤ 1.0
Residue on Evaporation: ≤ 0.0005%
Free Chlorine (Cl): ≤ 0.0001%
Iron (Fe): ≤ 0.00001%
Heavy Metals (as Pb): ≤ 0.000001%
Sulfate (SO₄): ≤ 0.0005%
Nitrate (NO₃): ≤ 0.0001%
Arsenic (As): ≤ 0.000001%
UN Number: UN 1789 (Hydrochloric acid)
Packaging Options: 500ml, 1L, 2.5L amber glass bottles

Applications

Analytical Titrations
Sample Preparation & Digestion
Quantitative Analysis
Elemental Analysis (ICP-MS/AAS)
Research & Development
University Laboratories
Quality Control Testing
Pharmaceutical Analysis
Environmental Testing
Food Analysis
Mineral Analysis
Reference Standards

Industry-Specific Grades

DRAVYOM offers specialized hydrochloric acid grades tailored for specific analytical requirements, ensuring optimal performance and regulatory compliance across diverse laboratory applications.

AR Grade (Analytical Reagent)
Assay: 35-37% Residue: ≤ 0.0005% Heavy Metals: ≤ 0.000001% Application: Analytical procedures, titrations
ACS Grade (Certified)
Assay: 36.5-38.0% Iron: ≤ 0.00001% Sulfate: ≤ 0.0005% Application: ACS certified analytical work
Trace Metal Grade
Assay: 34-37% Metal Impurities: ≤ 0.1 ppm each Total Metals: ≤ 1 ppm Application: ICP-MS, AAS analysis
Ultra-Pure Grade
Assay: 36-38% Particle Count: ≤ 50 per mL Organics: ≤ 1 ppm Application: Semiconductor, electronics

Quality Standards

DRAVYOM's AR Grade Hydrochloric Acid is manufactured under stringent quality control protocols, meeting international analytical standards including ACS, ISO, and pharmacopoeia specifications. Our analytical-grade production ensures consistent performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
ACS Grade Specifications
35-37% Accurate Concentration
Advanced Analytical Testing
Ultra-Low Metal Impurities
Batch-to-Batch Consistency
Traceable Certificate of Analysis
Temperature-Controlled Logistics

Advanced Chemical Properties & Performance

Coomassie Brilliant Blue exhibits exceptional protein-binding dye properties essential for protein analysis applications. Its specific protein interaction and staining capabilities ensure reliable performance in demanding electrophoresis protocols and protein quantification studies.

Dye Properties
Molecular Weight: 854.02 Da (G-250)
Dye Class: Triphenylmethane
Protein Binding: Ionic and hydrophobic interactions
Color Forms: Red (free) to Blue (bound)
Physical Properties
Appearance: Blue-violet powder
Solubility: Aqueous and alcoholic solutions
Absorption Maximum: 595 nm (protein-bound)
Extinction Coefficient: 43,000 M⁻¹cm⁻¹
Protein Analysis Performance
Detection Limit: 0.1-1 μg protein
Linear Range: 1-20 μg/mL
Binding Specificity: Basic and aromatic amino acids
Background Staining: Minimal interference
Purity Specifications
Dye Content: ≥ 90%
Protein Binding: Quantitative response
Heavy Metals: ≤ 50 ppm
Water Content: ≤ 5%
Stability Properties
Shelf Life: 5 years (room temperature)
Solution Stability: Weeks at 4°C
pH Stability: Stable pH 1-13
Light Sensitivity: Moderate photostability

Performance Characteristics

Detailed performance metrics demonstrate Coomassie Brilliant Blue superiority in protein analysis applications with exceptional binding specificity, detection sensitivity, and reproducibility across diverse protein quantification protocols and gel staining.

Detection Sensitivity

Protein detection: 0.1-1 μg

High sensitivity protein detection
Protein Binding

Binding sites: Arginine, lysine residues

Specific amino acid interaction
Linear Response

Quantification range: 1-20 μg/mL

Excellent linearity
Temperature Stability

Working range: 4-25°C

Stable across working temperatures
Batch Reproducibility

Staining variation: ±5%

Consistent dye performance
Stability Performance

5 years under proper storage

Extended dye integrity

Safety Information

Generally safe laboratory dye for research use. Handle with standard laboratory precautions including safety goggles, gloves, and lab coat. May cause staining of skin and clothing. Avoid inhalation of powder and direct contact with eyes. Non-toxic at normal usage levels.

Safe Laboratory Dye
May Cause Staining
Standard Lab Precautions

Storage & Handling

Store in original containers at room temperature in a dry environment. Keep containers tightly closed to prevent moisture absorption. Solutions can be stored at 4°C for extended periods. Protect from prolonged light exposure during storage.

Room temperature storage
Dry environment required
Protect from prolonged light
Stable under normal conditions

Chemical Mechanisms & Reaction Pathways

Coomassie Brilliant Blue binds to proteins through hydrophobic and electrostatic interactions, enabling precise protein quantification applications with predictable binding stoichiometry and quantitative colorimetric detection for biochemical analysis studies.

Protein Binding

Non-covalent binding to basic amino acid residues and hydrophobic regions

Essential for Bradford protein assays
Spectral Shift

λmax shift from 465nm to 595nm upon protein binding

Critical for quantitative protein detection
Gel Staining

Protein visualization through dye intercalation in gel matrices

Enables SDS-PAGE protein analysis
pH Sensitivity

pH-dependent binding affinity and color stability

Supporting assay optimization research

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global protein research laboratory access with complete documentation packages supporting international standards and protein analysis method validations.

Research Grade Standards

High purity specifications for protein research applications

ISO Standards

International standard compliance for research reagent specifications

Protein Analysis Grade

Protein analysis grade specifications for biochemical studies

Global Compliance

International protein research regulation compliance

Method Validation

Supported protein method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's protein research team provides comprehensive method support, troubleshooting assistance, and protein analysis services to optimize Coomassie Brilliant Blue performance in your specific research applications.

Method Development
  • Bradford assay optimization support
  • Gel staining protocol guidance
  • Protein quantification methods
  • Custom protein procedures
Research Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Dye binding testing
  • Protein analysis method validation support
Technical Support
  • Protein assay troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our Coomassie Brilliant Blue production emphasizes environmental responsibility through sustainable dye synthesis practices, waste minimization, and comprehensive environmental impact management for protein research operations.

Waste Minimization

Optimized dye synthesis with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient dye synthesis with emission controls

Safe Disposal

Comprehensive guidance for dye waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art dye manufacturing facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade Coomassie Brilliant Blue quality and performance across all production batches.

Production Process

Advanced dye synthesis and purification in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including spectroscopic and binding analysis

UV-Vis verification and protein binding testing
Quality Systems

ISO 9001:2015 quality management with protein research laboratory accreditation

Continuous improvement and method validation
Packaging Control

Light-protected containers with moisture control

Dye stability protection and contamination prevention

Market Applications & Performance Data

Comprehensive protein research data demonstrating Coomassie Brilliant Blue effectiveness across diverse applications with quantified performance metrics and protein analysis method validations.

Research Laboratories
Purity: >98% dye content Sensitivity: 10-100 μg protein detection Stability: 36+ months ambient storage
Academic Institutions
Publication Support: 5000+ cited protocols Reproducibility: >99% inter-lab agreement Innovation: Custom protein reagent solutions
Biotechnology Companies
Reliability: 99.9% batch consistency Quality Assurance: Validated protein binding Efficiency: Optimized Bradford assay protocols

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds research specifications with ultra-high purity and exceptional protein binding performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Protein Research Expertise

Dedicated protein research team provides assay optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed dye performance data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services