Calcium Chloride
Biotechnology Essential Salt
Biotechnology Reagents

Calcium Chloride

High-purity calcium chloride essential for biotechnology applications. Provides reliable calcium ions for cell culture media, transformation procedures, competent cell preparation, and biochemical research requiring precise ionic conditions.

  • High-Purity Essential Salt
  • Cell Culture Compatible
  • Transformation Procedures
  • Competent Cell Preparation
  • Molecular Biology Grade
  • Research Validated
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Technical Specifications

Chemical Name: Calcium Chloride Dihydrate
Molecular Formula: CaCl₂·2H₂O
CAS Number: 10035-04-8
Molecular Weight: 147.01 g/mol
Purity: ≥99.0% (Cell Culture Grade)
Physical Form: White crystalline powder
Solubility (25°C): 74.5 g/100mL water
pH (5% solution): 7.5-11.0
Heavy Metals: ≤ 5 ppm
Sulfate (SO₄): ≤ 0.005%
Storage Temperature: Room temperature
Packaging Options: 100g, 500g, 1kg bottles
Physical State: Clear, colorless liquid
Density (20°C): 1.18 g/cm³
Molecular Weight: 36.46 g/mol
Boiling Point: 108.6°C (azeotrope)
pH (1% solution): ≤ 1.0
Residue on Evaporation: ≤ 0.0005%
Free Chlorine (Cl): ≤ 0.0001%
Iron (Fe): ≤ 0.00001%
Heavy Metals (as Pb): ≤ 0.000001%
Sulfate (SO₄): ≤ 0.0005%
Nitrate (NO₃): ≤ 0.0001%
Arsenic (As): ≤ 0.000001%
UN Number: UN 1789 (Hydrochloric acid)
Packaging Options: 500ml, 1L, 2.5L amber glass bottles

Applications

Analytical Titrations
Sample Preparation & Digestion
Quantitative Analysis
Elemental Analysis (ICP-MS/AAS)
Research & Development
University Laboratories
Quality Control Testing
Pharmaceutical Analysis
Environmental Testing
Food Analysis
Mineral Analysis
Reference Standards

Industry-Specific Grades

DRAVYOM offers specialized hydrochloric acid grades tailored for specific analytical requirements, ensuring optimal performance and regulatory compliance across diverse laboratory applications.

AR Grade (Analytical Reagent)
Assay: 35-37% Residue: ≤ 0.0005% Heavy Metals: ≤ 0.000001% Application: Analytical procedures, titrations
ACS Grade (Certified)
Assay: 36.5-38.0% Iron: ≤ 0.00001% Sulfate: ≤ 0.0005% Application: ACS certified analytical work
Trace Metal Grade
Assay: 34-37% Metal Impurities: ≤ 0.1 ppm each Total Metals: ≤ 1 ppm Application: ICP-MS, AAS analysis
Ultra-Pure Grade
Assay: 36-38% Particle Count: ≤ 50 per mL Organics: ≤ 1 ppm Application: Semiconductor, electronics

Quality Standards

DRAVYOM's AR Grade Hydrochloric Acid is manufactured under stringent quality control protocols, meeting international analytical standards including ACS, ISO, and pharmacopoeia specifications. Our analytical-grade production ensures consistent performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
ACS Grade Specifications
35-37% Accurate Concentration
Advanced Analytical Testing
Ultra-Low Metal Impurities
Batch-to-Batch Consistency
Traceable Certificate of Analysis
Temperature-Controlled Logistics

Advanced Chemical Properties & Performance

Calcium Chloride exhibits exceptional divalent salt properties essential for molecular biology applications. Its high solubility and calcium ion availability ensure reliable performance in demanding transformation protocols and enzyme studies.

Ionic Properties
Dissociation: Complete ionic dissociation
Calcium Ion Source: Readily available Ca²⁺
Ionic Strength: High electrolyte activity
Hydration: Extensive water coordination
Physical Properties
Molecular Weight: 111.0 Da (anhydrous)
Solubility (25°C): 745 g/L in water
Crystal Structure: Cubic (dihydrate)
Hygroscopicity: Highly hygroscopic
Biochemical Performance
Transformation: Competent cell preparation
Enzyme Cofactor: Calcium-dependent enzymes
Cell Permeability: Membrane permeabilization
DNA Binding: Facilitates DNA uptake
Purity Specifications
Assay (dry basis): ≥ 99%
Heavy Metals: ≤ 10 ppm
Magnesium: ≤ 0.05%
Insoluble Matter: ≤ 0.01%
Stability Properties
Shelf Life: 5 years (dry storage)
Deliquescence: Absorbs moisture from air
Solution Stability: Indefinite at room temperature
pH Stability: Neutral aqueous solutions

Performance Characteristics

Detailed performance metrics demonstrate Calcium Chloride superiority in molecular biology applications with exceptional transformation efficiency, solubility, and reproducibility across diverse cloning protocols and enzyme studies.

Solubility Performance

Water solubility: 745 g/L at 25°C

Excellent aqueous dissolution
Transformation Efficiency

Competent cells: 10⁶-10⁸ CFU/μg DNA

High transformation rates
Enzyme Activation

Ca²⁺ cofactor: nM to mM range

Essential metal cofactor
Temperature Stability

Working range: 4-42°C

Stable across biological temperatures
Batch Reproducibility

Variation: ±1% between batches

Consistent salt performance
Stability Performance

5 years under proper storage

Extended salt integrity

Safety Information

Generally safe inorganic salt for research use. Handle with standard laboratory precautions including safety goggles, gloves, and lab coat. May cause mild irritation to eyes and skin. Hygroscopic - handle in dry conditions. Non-toxic at normal usage levels.

Generally Safe
Hygroscopic
Standard Lab Precautions

Storage & Handling

Store in original containers at room temperature in a dry environment. Keep containers tightly closed with desiccant to prevent moisture absorption. Solutions can be sterilized and stored at 4°C for extended periods. Use dry handling techniques.

Room temperature storage
Dry environment essential
Desiccant required
Protect from humidity

Chemical Mechanisms & Reaction Pathways

Calcium chloride provides essential Ca²⁺ ions for cellular signaling through calcium-dependent pathways, enabling precise molecular biology applications with predictable ionic strength and quantitative calcium availability for enzymatic and cellular studies.

Calcium Ion Release

Complete dissociation to provide bioavailable Ca²⁺ ions

Essential for calcium-dependent processes
Protein Interactions

Calcium binding to protein domains for structural stability

Critical for enzyme activation studies
Cell Membrane Effects

Calcium-mediated membrane permeabilization and transformation

Enables competent cell preparation
Nucleic Acid Interactions

DNA-calcium complex formation for transformation efficiency

Supporting molecular cloning research

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global molecular biology laboratory access with complete documentation packages supporting international standards and calcium-dependent method validations.

Research Grade Standards

High purity specifications for molecular biology applications

ISO Standards

International standard compliance for research reagent specifications

Molecular Biology Grade

Molecular biology grade specifications for calcium studies

Global Compliance

International molecular biology research regulation compliance

Method Validation

Supported calcium method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's molecular biology team provides comprehensive method support, troubleshooting assistance, and calcium-dependent services to optimize calcium chloride performance in your specific research applications.

Method Development
  • Competent cell protocol optimization support
  • Calcium concentration guidance
  • Transformation efficiency protocols
  • Custom calcium procedures
Research Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Calcium content testing
  • Molecular biology method validation support
Technical Support
  • Calcium troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our calcium chloride production emphasizes environmental responsibility through sustainable chemical synthesis practices, waste minimization, and comprehensive environmental impact management for molecular biology research operations.

Waste Minimization

Optimized salt production with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient synthesis with emission controls

Safe Disposal

Comprehensive guidance for salt waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art salt manufacturing facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade calcium chloride quality and performance across all production batches.

Production Process

Advanced crystallization and purification in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including ICP-MS and impurity analysis

Calcium content verification and trace metal analysis
Quality Systems

ISO 9001:2015 quality management with molecular biology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Moisture-protected containers with desiccant packaging

Hygroscopic protection and contamination prevention

Market Applications & Performance Data

Comprehensive molecular biology research data demonstrating calcium chloride effectiveness across diverse applications with quantified performance metrics and calcium-dependent method validations.

Research Laboratories
Purity: >99% calcium chloride content Activity: Confirmed transformation efficiency Stability: 36+ months ambient storage
Academic Institutions
Publication Support: 1500+ cited protocols Reproducibility: >99% inter-lab agreement Innovation: Custom calcium solutions
Biotechnology Companies
Reliability: 99.8% batch consistency Quality Assurance: 100% specification compliance Efficiency: Optimized competent cell protocols

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds research specifications with ultra-high purity and exceptional calcium performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Molecular Biology Expertise

Dedicated molecular biology team provides calcium optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed calcium content data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services