Bromophenol Blue Tracking Dye
Biotechnology Tracking Dye
Electrophoresis Reagents

Bromophenol Blue (Tracking Dye)

High-quality bromophenol blue tracking dye essential for electrophoresis applications. Provides clear visual tracking of DNA/RNA samples during gel electrophoresis, enabling precise monitoring of migration progress.

  • High-Quality Tracking Dye
  • Visual Sample Tracking
  • Electrophoresis Compatible
  • DNA/RNA Applications
  • Molecular Biology Grade
  • Research Validated
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Technical Specifications

Chemical Name: Bromophenol Blue
Molecular Formula: C₁₉H₁₀Br₄O₅S
CAS Number: 115-39-9
Molecular Weight: 669.96 g/mol
Purity: ≥95.0% (Electrophoresis Grade)
Physical Form: Dark blue crystalline powder
λmax (Absorption): 590 nm
pH Transition: 3.0-4.6 (yellow to blue)
Solubility: 0.4% in water, soluble in ethanol
Working Concentration: 0.025-0.1% in loading buffer
Storage Temperature: Room temperature
Packaging Options: 1g, 5g, 25g bottles
Concentration (Assay): 35-37% (ACS Grade)
Physical State: Clear, colorless liquid
Density (20°C): 1.18 g/cm³
Molecular Weight: 36.46 g/mol
Boiling Point: 108.6°C (azeotrope)
pH (1% solution): ≤ 1.0
Residue on Evaporation: ≤ 0.0005%
Free Chlorine (Cl): ≤ 0.0001%
Iron (Fe): ≤ 0.00001%
Heavy Metals (as Pb): ≤ 0.000001%
Sulfate (SO₄): ≤ 0.0005%
Nitrate (NO₃): ≤ 0.0001%
Arsenic (As): ≤ 0.000001%
UN Number: UN 1789 (Hydrochloric acid)
Packaging Options: 500ml, 1L, 2.5L amber glass bottles

Applications

Analytical Titrations
Sample Preparation & Digestion
Quantitative Analysis
Elemental Analysis (ICP-MS/AAS)
Research & Development
University Laboratories
Quality Control Testing
Pharmaceutical Analysis
Environmental Testing
Food Analysis
Mineral Analysis
Reference Standards

Industry-Specific Grades

DRAVYOM offers specialized hydrochloric acid grades tailored for specific analytical requirements, ensuring optimal performance and regulatory compliance across diverse laboratory applications.

AR Grade (Analytical Reagent)
Assay: 35-37% Residue: ≤ 0.0005% Heavy Metals: ≤ 0.000001% Application: Analytical procedures, titrations
ACS Grade (Certified)
Assay: 36.5-38.0% Iron: ≤ 0.00001% Sulfate: ≤ 0.0005% Application: ACS certified analytical work
Trace Metal Grade
Assay: 34-37% Metal Impurities: ≤ 0.1 ppm each Total Metals: ≤ 1 ppm Application: ICP-MS, AAS analysis
Ultra-Pure Grade
Assay: 36-38% Particle Count: ≤ 50 per mL Organics: ≤ 1 ppm Application: Semiconductor, electronics

Quality Standards

DRAVYOM's AR Grade Hydrochloric Acid is manufactured under stringent quality control protocols, meeting international analytical standards including ACS, ISO, and pharmacopoeia specifications. Our analytical-grade production ensures consistent performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
ACS Grade Specifications
35-37% Accurate Concentration
Advanced Analytical Testing
Ultra-Low Metal Impurities
Batch-to-Batch Consistency
Traceable Certificate of Analysis
Temperature-Controlled Logistics

Advanced Chemical Properties & Performance

Bromophenol Blue exhibits exceptional indicator dye properties essential for electrophoresis applications. Its pH-sensitive color change and tracking capabilities ensure reliable performance in demanding gel electrophoresis protocols and protein analysis.

Indicator Properties
Molecular Weight: 669.96 Da
Transition Range: pH 3.0-4.6
Color Change: Yellow to blue
pKa: 4.0
Physical Properties
Appearance: Dark blue powder
Solubility (water): 1.6 g/L at 25°C
Absorption Maximum: 590 nm (alkaline)
Extinction Coefficient: 41,000 M⁻¹cm⁻¹
Electrophoretic Performance
Tracking Efficiency: Migrates with 10-15 kDa proteins
Gel Compatibility: SDS-PAGE, Native PAGE
Mobility: Anionic at physiological pH
Visibility: Excellent visual tracking
Purity Specifications
Dye Content: ≥ 95%
pH Indicator Range: 3.0-4.6 (sharp transition)
Heavy Metals: ≤ 50 ppm
Insoluble Matter: ≤ 0.1%
Stability Properties
Shelf Life: 5 years (room temperature)
Light Sensitivity: Stable under laboratory light
Solution Stability: Months at 4°C
pH Stability: Stable across pH range

Performance Characteristics

Detailed performance metrics demonstrate Bromophenol Blue superiority in electrophoresis applications with exceptional tracking visibility, pH sensitivity, and reproducibility across diverse gel electrophoresis protocols and protein analysis.

Visual Tracking

Visibility: Excellent blue color

Clear migration tracking
Electrophoretic Mobility

Migration: 10-15 kDa equivalent

Predictable gel migration
pH Indication

Transition: pH 3.0-4.6

Sharp color transition
Temperature Stability

Working range: 4-37°C

Stable across working temperatures
Batch Reproducibility

Color variation: ±2%

Consistent dye performance
Stability Performance

5 years under proper storage

Extended dye integrity

Safety Information

Generally safe laboratory dye for research use. Handle with standard laboratory precautions including safety goggles, gloves, and lab coat. May cause staining of skin and clothing. Avoid inhalation of powder and direct contact with eyes. Non-toxic at normal usage levels.

Safe Laboratory Dye
May Cause Staining
Standard Lab Precautions

Storage & Handling

Store in original containers at room temperature in a dry environment. Keep containers tightly closed to prevent moisture absorption. Solutions can be stored at 4°C for extended periods. Stable under normal laboratory conditions.

Room temperature storage
Dry environment required
Original containers tightly closed
Stable under normal conditions

Chemical Mechanisms & Reaction Pathways

Bromophenol blue exhibits pH-dependent color changes through protonation equilibrium, enabling precise electrophoresis tracking applications with predictable migration behavior and quantitative gel analysis for molecular biology studies.

pH Indicator Chemistry

Yellow to blue color transition through protonation state changes

Essential for gel electrophoresis tracking
Migration Properties

Controlled electrophoretic mobility for gel front tracking

Critical for molecular weight estimation
Chromophore System

Triphenylmethane dye structure for visible detection

Enables visual gel monitoring
Ionic Interactions

Charge distribution effects on gel matrix migration

Supporting electrophoresis optimization

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global molecular biology laboratory access with complete documentation packages supporting international standards and electrophoresis method validations.

Research Grade Standards

High purity specifications for molecular biology applications

ISO Standards

International standard compliance for research reagent specifications

Electrophoresis Grade

Electrophoresis grade specifications for gel applications

Global Compliance

International molecular biology research regulation compliance

Method Validation

Supported electrophoresis method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's molecular biology team provides comprehensive method support, troubleshooting assistance, and electrophoresis services to optimize bromophenol blue performance in your specific research applications.

Method Development
  • Gel electrophoresis optimization support
  • Loading dye formulation guidance
  • Migration tracking protocols
  • Custom electrophoresis procedures
Research Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Dye stability testing
  • Electrophoresis method validation support
Technical Support
  • Electrophoresis troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our bromophenol blue production emphasizes environmental responsibility through sustainable chemical synthesis practices, waste minimization, and comprehensive environmental impact management for molecular biology research operations.

Waste Minimization

Optimized dye synthesis with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient dye synthesis with emission controls

Safe Disposal

Comprehensive guidance for dye waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art dye manufacturing facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade bromophenol blue quality and performance across all production batches.

Production Process

Advanced dye synthesis and purification in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including spectroscopic and migration analysis

UV-Vis verification and electrophoretic testing
Quality Systems

ISO 9001:2015 quality management with molecular biology laboratory accreditation

Continuous improvement and method validation
Packaging Control

Light-protected containers with moisture control

Dye stability protection and contamination prevention

Market Applications & Performance Data

Comprehensive molecular biology research data demonstrating bromophenol blue effectiveness across diverse applications with quantified performance metrics and electrophoresis method validations.

Research Laboratories
Purity: >99% dye content Migration: Consistent electrophoretic mobility Stability: 36+ months ambient storage
Academic Institutions
Publication Support: 2000+ cited protocols Reproducibility: >99% inter-lab agreement Innovation: Custom loading dye solutions
Biotechnology Companies
Reliability: 99.9% batch consistency Quality Assurance: Validated gel performance Efficiency: Optimized electrophoresis protocols

DRAVYOM Competitive Advantages

Superior Purity

Consistently exceeds research specifications with ultra-high purity and exceptional dye performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Molecular Biology Expertise

Dedicated molecular biology team provides electrophoresis optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed dye performance data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services