Beta-Lactamase Enzyme
Biotechnology Premium
Research Enzyme

Beta-Lactamase Enzyme

High-quality Beta-Lactamase enzyme for antibiotic resistance research, molecular biology studies, and biochemical assays. Premium grade enzyme manufactured for research applications and antibiotic studies.

  • Antibiotic Resistance Research
  • High Enzymatic Activity
  • Biochemical Assay Compatible
  • Molecular Biology Applications
  • Research Grade Purity
  • Stable Storage Conditions
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Technical Specifications

Enzyme Name: β-Lactamase (TEM-1)
EC Number: 3.5.2.6
CAS Number: 9001-74-5
Molecular Weight: 28.9 kDa
Activity: ≥2000 units/mg protein
Physical Form: Lyophilized powder
pH Optimum: 7.0-8.0
Temperature Optimum: 25-37°C
Storage Temperature: -20°C
Stability: ≥12 months at -20°C
Substrate Specificity: Penicillins, cephalosporins
Packaging Options: 100 units, 500 units, 1000 units
Boiling Point: 108.6°C (azeotrope)
pH (1% solution): ≤ 1.0
Residue on Evaporation: ≤ 0.0005%
Free Chlorine (Cl): ≤ 0.0001%
Iron (Fe): ≤ 0.00001%
Heavy Metals (as Pb): ≤ 0.000001%
Sulfate (SO₄): ≤ 0.0005%
Nitrate (NO₃): ≤ 0.0001%
Arsenic (As): ≤ 0.000001%
UN Number: UN 1789 (Hydrochloric acid)
Packaging Options: 500ml, 1L, 2.5L amber glass bottles

Applications

Analytical Titrations
Sample Preparation & Digestion
Quantitative Analysis
Elemental Analysis (ICP-MS/AAS)
Research & Development
University Laboratories
Quality Control Testing
Pharmaceutical Analysis
Environmental Testing
Food Analysis
Mineral Analysis
Reference Standards

Industry-Specific Grades

DRAVYOM offers specialized hydrochloric acid grades tailored for specific analytical requirements, ensuring optimal performance and regulatory compliance across diverse laboratory applications.

AR Grade (Analytical Reagent)
Assay: 35-37% Residue: ≤ 0.0005% Heavy Metals: ≤ 0.000001% Application: Analytical procedures, titrations
ACS Grade (Certified)
Assay: 36.5-38.0% Iron: ≤ 0.00001% Sulfate: ≤ 0.0005% Application: ACS certified analytical work
Trace Metal Grade
Assay: 34-37% Metal Impurities: ≤ 0.1 ppm each Total Metals: ≤ 1 ppm Application: ICP-MS, AAS analysis
Ultra-Pure Grade
Assay: 36-38% Particle Count: ≤ 50 per mL Organics: ≤ 1 ppm Application: Semiconductor, electronics

Quality Standards

DRAVYOM's AR Grade Hydrochloric Acid is manufactured under stringent quality control protocols, meeting international analytical standards including ACS, ISO, and pharmacopoeia specifications. Our analytical-grade production ensures consistent performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
ACS Grade Specifications
35-37% Accurate Concentration
Advanced Analytical Testing
Ultra-Low Metal Impurities
Batch-to-Batch Consistency
Traceable Certificate of Analysis
Temperature-Controlled Logistics

Advanced Chemical Properties & Performance

β-Lactamase exhibits exceptional enzyme properties essential for antibiotic resistance studies. Its specific substrate recognition and catalytic efficiency ensure reliable performance in demanding microbiology protocols and resistance mechanism research.

Enzyme Properties
Molecular Weight: 29 kDa (TEM-1)
Enzyme Class: EC 3.5.2.6
Substrate Specificity: β-lactam antibiotics
Catalytic Mechanism: Serine-based hydrolysis
Physical Properties
Optimal pH: 7.0-8.0
Optimal Temperature: 37°C
Isoelectric Point: 5.4
Thermal Stability: Stable up to 60°C
Biochemical Performance
Km (penicillin G): 20 μM
Turnover Number: 2,000 s⁻¹
Catalytic Efficiency: 1.0 × 10⁸ M⁻¹s⁻¹
Inhibitors: Clavulanic acid, EDTA
Purity Specifications
Enzyme Activity: ≥ 2,000 U/mg
Protein Content: ≥ 90%
Contaminating Enzymes: < 0.1%
Endotoxin: < 10 EU/mg
Stability Properties
Storage Stability: 2 years at -20°C
Working Solution: 1 week at 4°C
pH Stability: pH 6.0-9.0
Salt Stability: Stable up to 1M NaCl

Performance Characteristics

Detailed performance metrics demonstrate β-Lactamase superiority in antibiotic resistance studies with exceptional catalytic efficiency, substrate specificity, and reproducibility across diverse microbiology protocols and resistance research.

Catalytic Efficiency

kcat/Km: 1.0 × 10⁸ M⁻¹s⁻¹

Extremely efficient antibiotic hydrolysis
Resistance Activity

Penicillin resistance: >1000-fold

High-level antibiotic resistance
Substrate Range

β-lactam spectrum: Penicillins, some cephalosporins

Broad substrate recognition
Temperature Stability

Working range: 25-60°C

Stable across working temperatures
Batch Reproducibility

Activity variation: ±5%

Consistent enzyme performance
Stability Performance

2 years under proper storage

Extended enzyme integrity

Safety Information

Generally safe recombinant enzyme for research use. Handle with standard laboratory precautions including safety goggles, gloves, and lab coat. Protein allergen potential - avoid inhalation and skin contact. Enzyme inactivates antibiotics - handle with care in clinical settings.

Safe Recombinant Enzyme
Protein Allergen
Standard Lab Precautions

Storage & Handling

Store in original containers at -20°C in a dry environment. Keep containers tightly closed with glycerol storage buffer. Avoid repeated freeze-thaw cycles. Prepare working solutions in appropriate buffer at 4°C for short-term use.

Frozen storage (-20°C)
Glycerol storage buffer
Avoid repeated freeze-thaw
Keep working solutions at 4°C

Chemical Mechanisms & Reaction Pathways

β-Lactamase catalyzes β-lactam ring hydrolysis through nucleophilic attack, enabling precise antibiotic resistance research applications with predictable enzymatic activity and quantitative substrate conversion for drug discovery studies.

β-Lactam Hydrolysis

β-Lactam ring cleavage through serine nucleophilic attack mechanism

Essential for antibiotic resistance studies
Substrate Specificity

Penicillin and cephalosporin substrate recognition and binding

Critical for drug resistance analysis
Enzyme Kinetics

Rapid turnover kinetics for antibiotic inactivation

Enables resistance mechanism studies
Active Site Chemistry

Serine residue nucleophilic catalysis with metal cofactors

Supporting enzyme structure research

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global biochemical research laboratory access with complete documentation packages supporting international standards and enzyme research method validations.

Research Grade Standards

High activity specifications for biochemical research applications

ISO Standards

International standard compliance for research reagent specifications

Enzyme Research Grade

Enzyme research grade specifications for antibiotic studies

Global Compliance

International biochemical research regulation compliance

Method Validation

Supported enzyme method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's enzyme research team provides comprehensive method support, troubleshooting assistance, and enzyme services to optimize β-lactamase performance in your specific research applications.

Method Development
  • Enzyme assay optimization support
  • Activity measurement guidance
  • Substrate preparation protocols
  • Custom resistance assay procedures
Research Services
  • Certificate of Analysis verification
  • Custom activity analysis
  • Enzyme stability testing
  • Antibiotic research method validation support
Technical Support
  • Enzyme troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our β-lactamase production emphasizes environmental responsibility through sustainable biotechnology practices, waste minimization, and comprehensive environmental impact management for enzyme research operations.

Waste Minimization

Optimized biotechnology production with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient enzyme production with emission controls

Safe Disposal

Comprehensive guidance for enzyme waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art enzyme manufacturing facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade β-lactamase quality and performance across all production batches.

Production Process

Advanced recombinant expression and purification in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including enzyme activity and specificity analysis

Activity verification and substrate specificity testing
Quality Systems

ISO 9001:2015 quality management with enzyme research laboratory accreditation

Continuous improvement and method validation
Packaging Control

Temperature-controlled containers with stabilizing buffers

Enzyme activity preservation and contamination prevention

Market Applications & Performance Data

Comprehensive enzyme research data demonstrating β-lactamase effectiveness across diverse applications with quantified performance metrics and antibiotic resistance method validations.

Research Laboratories
Activity: >500 U/mg protein Specificity: Broad β-lactam substrate range Stability: 18+ months at -20°C
Academic Institutions
Publication Support: 400+ cited studies Reproducibility: >98% inter-lab agreement Innovation: Custom enzyme solutions
Pharmaceutical Companies
Reliability: 99.5% batch consistency Quality Assurance: Validated enzyme activity Efficiency: Optimized resistance assays

DRAVYOM Competitive Advantages

Superior Activity

Consistently exceeds research specifications with ultra-high activity and exceptional enzyme performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Enzyme Expertise

Dedicated enzyme research team provides optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed enzyme activity data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services