Beta-Galactosidase Enzyme
Biotechnology Premium
Reporter Enzyme

Beta-Galactosidase Enzyme

High-quality Beta-Galactosidase enzyme for molecular biology research, gene expression studies, and colorimetric assays. Premium grade LacZ reporter enzyme manufactured for research applications and expression analysis.

  • LacZ Reporter System
  • Gene Expression Analysis
  • Colorimetric Detection
  • High Enzymatic Activity
  • Research Grade Purity
  • Molecular Biology Standard
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Technical Specifications

Enzyme Name: β-Galactosidase (E. coli)
EC Number: 3.2.1.23
CAS Number: 9031-11-2
Molecular Weight: 464.3 kDa (tetramer)
Activity: ≥500 units/mg protein
Physical Form: Lyophilized powder
pH Optimum: 7.0-7.5
Temperature Optimum: 37°C
Storage Temperature: -20°C
Buffer Compatibility: Tris-HCl, PBS, sodium phosphate
Substrate: X-gal, ONPG, lactose
Packaging Options: 100 units, 500 units, 1000 units
Boiling Point: 108.6°C (azeotrope)
pH (1% solution): ≤ 1.0
Residue on Evaporation: ≤ 0.0005%
Free Chlorine (Cl): ≤ 0.0001%
Iron (Fe): ≤ 0.00001%
Heavy Metals (as Pb): ≤ 0.000001%
Sulfate (SO₄): ≤ 0.0005%
Nitrate (NO₃): ≤ 0.0001%
Arsenic (As): ≤ 0.000001%
UN Number: UN 1789 (Hydrochloric acid)
Packaging Options: 500ml, 1L, 2.5L amber glass bottles

Applications

Analytical Titrations
Sample Preparation & Digestion
Quantitative Analysis
Elemental Analysis (ICP-MS/AAS)
Research & Development
University Laboratories
Quality Control Testing
Pharmaceutical Analysis
Environmental Testing
Food Analysis
Mineral Analysis
Reference Standards

Industry-Specific Grades

DRAVYOM offers specialized hydrochloric acid grades tailored for specific analytical requirements, ensuring optimal performance and regulatory compliance across diverse laboratory applications.

AR Grade (Analytical Reagent)
Assay: 35-37% Residue: ≤ 0.0005% Heavy Metals: ≤ 0.000001% Application: Analytical procedures, titrations
ACS Grade (Certified)
Assay: 36.5-38.0% Iron: ≤ 0.00001% Sulfate: ≤ 0.0005% Application: ACS certified analytical work
Trace Metal Grade
Assay: 34-37% Metal Impurities: ≤ 0.1 ppm each Total Metals: ≤ 1 ppm Application: ICP-MS, AAS analysis
Ultra-Pure Grade
Assay: 36-38% Particle Count: ≤ 50 per mL Organics: ≤ 1 ppm Application: Semiconductor, electronics

Quality Standards

DRAVYOM's AR Grade Hydrochloric Acid is manufactured under stringent quality control protocols, meeting international analytical standards including ACS, ISO, and pharmacopoeia specifications. Our analytical-grade production ensures consistent performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
ACS Grade Specifications
35-37% Accurate Concentration
Advanced Analytical Testing
Ultra-Low Metal Impurities
Batch-to-Batch Consistency
Traceable Certificate of Analysis
Temperature-Controlled Logistics

Advanced Chemical Properties & Performance

β-Galactosidase exhibits exceptional enzyme properties essential for molecular biology applications. Its specific substrate recognition and catalytic efficiency ensure reliable performance in demanding cloning protocols and gene expression studies.

Enzyme Properties
Molecular Weight: 464 kDa (tetrameric)
Subunit Structure: 116 kDa monomers
Substrate Specificity: β-galactosides (lactose, X-gal)
Km (lactose): 4.0 mM
Physical Properties
Optimal pH: 7.5
Optimal Temperature: 37°C
Isoelectric Point: 4.6
Thermal Stability: Stable up to 50°C
Biochemical Performance
Turnover Number: 350 s⁻¹
Catalytic Efficiency: 8.8 × 10⁴ M⁻¹s⁻¹
Metal Requirement: Mg²⁺, Na⁺
Inhibitors: Glucose, galactose
Purity Specifications
Enzyme Activity: ≥ 300 U/mg
Protein Content: ≥ 90%
Contaminating Enzymes: < 0.1%
Endotoxin: < 10 EU/mg
Stability Properties
Storage Stability: 2 years at -20°C
Working Solution: 1 week at 4°C
Freeze-thaw Cycles: Stable up to 5 cycles
pH Stability: pH 6.0-8.0

Performance Characteristics

Detailed performance metrics demonstrate β-Galactosidase superiority in molecular biology applications with exceptional catalytic efficiency, substrate specificity, and reproducibility across diverse cloning protocols and gene expression studies.

Catalytic Efficiency

kcat/Km: 8.8 × 10⁴ M⁻¹s⁻¹

Highly efficient substrate conversion
Substrate Recognition

X-gal cleavage: Blue color formation

Specific chromogenic reaction
Cloning Efficiency

Blue/white screening: >95% accuracy

Reliable recombinant detection
Temperature Stability

Working range: 25-45°C

Stable across working temperatures
Batch Reproducibility

Activity variation: ±10%

Consistent enzyme performance
Stability Performance

2 years under proper storage

Extended enzyme integrity

Safety Information

Generally safe recombinant enzyme for research use. Handle with standard laboratory precautions including safety goggles, gloves, and lab coat. Protein allergen potential - avoid inhalation and skin contact. Non-toxic and biodegradable. Follow proper biotechnology safety protocols.

Safe Recombinant Enzyme
Protein Allergen
Standard Lab Precautions

Storage & Handling

Store in original containers at -20°C in a dry environment. Keep containers tightly closed with glycerol storage buffer. Avoid repeated freeze-thaw cycles. Prepare working solutions in appropriate buffer at 4°C for short-term use.

Frozen storage (-20°C)
Glycerol storage buffer
Avoid repeated freeze-thaw
Keep working solutions at 4°C

Chemical Mechanisms & Reaction Pathways

β-Galactosidase catalyzes galactose liberation from lactose through hydrolytic cleavage, enabling precise molecular biology applications with predictable enzymatic activity and quantitative substrate conversion for gene expression studies.

Hydrolytic Cleavage

Lactose → galactose + glucose through β-galactosidase activity

Essential for lac operon studies
Substrate Specificity

ONPG and X-gal substrate hydrolysis for colorimetric detection

Critical for gene expression analysis
Enzyme Kinetics

Michaelis-Menten kinetics for quantitative enzyme analysis

Enables activity measurement studies
Metal Ion Cofactors

Mg²⁺ and Na⁺ requirement for optimal enzyme activity

Supporting enzyme structure research

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global molecular biology laboratory access with complete documentation packages supporting international standards and enzyme research method validations.

Research Grade Standards

High activity specifications for molecular biology applications

ISO Standards

International standard compliance for research reagent specifications

Molecular Biology Grade

Molecular biology grade specifications for gene expression studies

Global Compliance

International molecular biology research regulation compliance

Method Validation

Supported enzyme method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's enzyme research team provides comprehensive method support, troubleshooting assistance, and enzyme services to optimize β-galactosidase performance in your specific research applications.

Method Development
  • Enzyme assay optimization support
  • Activity measurement guidance
  • Substrate preparation protocols
  • Custom assay procedures
Research Services
  • Certificate of Analysis verification
  • Custom activity analysis
  • Enzyme stability testing
  • Molecular biology method validation support
Technical Support
  • Enzyme troubleshooting consultation
  • Storage and handling guidance
  • Laboratory safety protocols
  • Research best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom packaging options
  • Global distribution network

Environmental Impact & Sustainability

Our β-galactosidase production emphasizes environmental responsibility through sustainable biotechnology practices, waste minimization, and comprehensive environmental impact management for enzyme research operations.

Waste Minimization

Optimized biotechnology production with minimal waste generation

Water Treatment

Advanced wastewater treatment and recycling systems

Green Production

Energy-efficient enzyme production with emission controls

Safe Disposal

Comprehensive guidance for enzyme waste management

ISO 14001

Environmental management system certified production

Container Recycling

Sustainable container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art enzyme manufacturing facility employs advanced purification technology and continuous monitoring systems to ensure consistent research-grade β-galactosidase quality and performance across all production batches.

Production Process

Advanced recombinant expression and purification in controlled environment

Multi-stage purification for research grade quality
Quality Testing

Comprehensive analytical testing including enzyme activity and purity analysis

Activity verification and protein analysis
Quality Systems

ISO 9001:2015 quality management with enzyme research laboratory accreditation

Continuous improvement and method validation
Packaging Control

Temperature-controlled containers with glycerol stabilizers

Enzyme activity preservation and contamination prevention

Market Applications & Performance Data

Comprehensive molecular biology research data demonstrating β-galactosidase effectiveness across diverse applications with quantified performance metrics and enzyme method validations.

Research Laboratories
Activity: >1000 U/mg protein Specificity: High substrate selectivity Stability: 12+ months at -20°C
Academic Institutions
Publication Support: 600+ cited studies Reproducibility: >99% inter-lab agreement Innovation: Custom enzyme solutions
Biotechnology Companies
Reliability: 99.7% batch consistency Quality Assurance: Validated enzyme activity Efficiency: Optimized assay protocols

DRAVYOM Competitive Advantages

Superior Activity

Consistently exceeds research specifications with ultra-high activity and exceptional enzyme performance

Reliable Supply

Guaranteed availability with strategic inventory management and research-grade production scheduling

Enzyme Expertise

Dedicated enzyme research team provides optimization and troubleshooting support

Quality Assurance

Comprehensive certificates with detailed enzyme activity data and method validation support

Global Standards

International compliance with research specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research laboratories and custom grade development services