AR Grade TBE Buffer
Analytical Buffer Grade
Analytical Reagent

AR Grade TBE Buffer

Ultra-pure Analytical Reagent grade TBE Buffer specifically formulated for precision electrophoresis applications. Our AR grade buffer delivers exceptional pH stability and consistent ionic strength for critical DNA separation and analytical molecular biology applications requiring the highest buffer quality.

  • pH 8.3 ± 0.05 Precision
  • 10X Analytical Concentrate
  • Exceptional Ionic Strength
  • Analytical Grade Quality
  • Analytical Reagent Standard
  • Ultra-Pure Format
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Technical Specifications

Buffer Name: Tris-Borate-EDTA (TBE)
Concentration: 10X concentrate
Tris Base: 108 g/L (890 mM)
Boric Acid: 55 g/L (890 mM)
EDTA: 9.3 g/L (25 mM)
pH (1X diluted): 8.3 ± 0.1
Ionic Strength: 89 mM (1X)
Conductivity: 8.5 mS/cm (1X)
Physical State: Clear, colorless solution
Sterility: 0.2 μm filtered
Endotoxin Level: ≤ 1 EU/mL
DNase/RNase: Not detected
Storage Temperature: Room temperature
Package Sizes: 500mL, 1L bottles
Shelf Life: 2 years at room temperature
Grade: Analytical Reagent (A.R.)
Working Dilution: 1:10 with ultrapure water
Buffer Capacity: High buffering at pH 8.3

Applications

DNA Electrophoresis
Agarose Gel Running
Polyacrylamide Gels
Nucleic Acid Analysis
Molecular Weight Determination
Academic Research
Biotechnology Applications
Clinical Diagnostics
Plant Genomics
Viral Detection
Quality Control Testing
Molecular Cloning

Industry-Specific Grades

DRAVYOM offers specialized TBE buffer formulations tailored for specific electrophoresis applications, ensuring optimal DNA migration and resolution with controlled ionic strength and pH stability for precise molecular analysis.

Standard TBE
Concentration: 10X pH: 8.3 ± 0.1 Ionic Strength: 89 mM Application: Routine electrophoresis
Ultra-Pure TBE
Concentration: 10X DNase/RNase Free: Guaranteed Endotoxin: ≤ 0.1 EU/mL Application: Critical applications
Ready-to-Use TBE
Concentration: 1X pH: Pre-adjusted Convenience: Ready-to-use Application: Quick analysis
Clinical Grade
Concentration: 10X Sterility: Validated Traceability: Full documentation Application: Diagnostic labs

Quality Standards

DRAVYOM's TBE buffer is manufactured under stringent quality control protocols, meeting international molecular biology standards including ISO specifications and laboratory requirements for reliable electrophoresis applications.

ISO 9001:2015 Certified Manufacturing
Molecular Biology Grade
pH 8.3 ± 0.1 Precision
Electrophoresis Performance Testing
DNase/RNase Free
0.2 μm Sterile Filtered
Buffer Performance Certificate
Stable Room Temperature Storage

Advanced Chemical Properties & Performance

AR Grade TBE Buffer exhibits exceptional chemical properties essential for electrophoresis applications. Its ultra-pure composition and precise pH buffering ensure reliable performance in demanding gel electrophoresis procedures and research applications.

Buffer Properties
Buffer pH (1X): 8.3 ± 0.1
Ionic Strength: 89 mM
Conductivity (1X): 8.5 mS/cm
Buffer Capacity: 45 mmol/L·pH
Physical Properties
Osmolality (1X): 178 mOsm/kg
Density (20°C): 1.005 g/cm³
Viscosity: 1.02 mPa·s
Temperature Coefficient: -0.02 pH/°C
Electrophoresis Performance
Resolution: High-resolution separation
Migration Rate: Optimal DNA mobility
Band Sharpness: Excellent definition
Background Noise: Minimal interference
Purity Specifications
Total Impurities: ≤ 0.001%
Heavy Metals: ≤ 0.1 ppm
DNase/RNase Activity: Non-detectable
Endotoxin Level: ≤ 0.1 EU/ml
Stability Properties
Shelf Life: 2 years at room temp
Working Solution Stability: 6 months at 4°C
pH Stability: ±0.05 pH over time
Autoclave Compatibility: Stable at 121°C

Performance Characteristics

Detailed performance metrics demonstrate AR Grade TBE Buffer superiority in electrophoresis applications with exceptional resolution, stability, and reproducibility across diverse gel electrophoresis procedures.

DNA Separation

Resolution: Superior band separation

Exceptional electrophoretic clarity
pH Stability

Buffer range: pH 8.3 ± 0.1 maintained

Superior buffering capacity
Conductivity

Conductance: 8.5 mS/cm optimal

Consistent electrical properties
Run Time

Efficiency: Reduced electrophoresis time

Fast, efficient separation
Batch Reproducibility

Variation: ±2% between batches

Exceptional lot-to-lot consistency
Nuclease-Free

Activity: No DNase/RNase detected

Guaranteed nucleic acid integrity

Safety Information

Contains boric acid - handle with standard laboratory precautions. Use appropriate protective equipment including safety goggles, lab coats, and gloves. Ensure proper ventilation during use. Avoid skin and eye contact. Follow standard laboratory safety protocols.

Mild Irritant
Eye Protection
Avoid Skin Contact

Storage & Handling

Store at room temperature in original containers. Keep away from strong acids and bases. Use sterile techniques when preparing working solutions. Autoclave if sterilization is required. Prepare fresh working solutions for optimal performance.

Room temperature storage
Sterile preparation techniques
Original containers only
Autoclave compatible

Chemical Mechanisms & Reaction Pathways

TBE Buffer exhibits complex buffering mechanisms through Tris-boric acid equilibria, enabling precise electrophoresis conditions with predictable ionic strength and pH stability for nucleic acid separation applications.

Buffer Equilibria

Tris-H⁺ ⇌ Tris + H⁺ and H₃BO₃ ⇌ H₂BO₃⁻ + H⁺ buffering system

Dual buffering for pH stability
Ion Mobility

Controlled ionic environment for consistent DNA migration

Predictable electrophoretic behavior
EDTA Chelation

Metal ion sequestration to prevent nuclease activity

DNA/RNA protection mechanism
Conductivity Control

Optimized ionic strength for uniform electric field

Enhanced separation resolution

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global molecular biology laboratory access with complete documentation packages supporting international standards and electrophoresis method validations.

ACS Specifications

American Chemical Society molecular biology buffer standards

ISO 6353 Standard

International standard for electrophoresis buffer specifications

Molecular Biology Grade

Ultra-pure specifications for nucleic acid applications

REACH Registration

European Union chemical regulation compliance

Method Validation

Supported electrophoresis method validation documentation

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's molecular biology team provides comprehensive electrophoresis support, troubleshooting assistance, and optimization services to maximize TBE Buffer performance in your specific separation applications.

Method Development
  • Electrophoresis optimization protocols
  • Gel preparation guidance
  • Voltage/time optimization
  • Custom separation procedures
Analytical Services
  • Buffer pH verification
  • Ionic strength testing
  • Nuclease contamination testing
  • Performance validation
Technical Support
  • Electrophoresis troubleshooting
  • Protocol optimization guidance
  • Safety training and protocols
  • Molecular biology best practices
Supply Solutions
  • Consistent batch scheduling
  • Emergency supply arrangements
  • Custom concentration preparation
  • Global distribution network

Environmental Impact & Sustainability

Our TBE Buffer production emphasizes environmental responsibility through sustainable manufacturing practices, buffer recovery systems, and comprehensive environmental impact management for molecular biology operations.

Buffer Recovery

Advanced buffer solution recovery and purification systems

Water Conservation

Closed-loop water treatment and recycling systems

Clean Production

Energy-efficient buffer preparation with emission controls

Safe Disposal

Comprehensive guidance for electrophoresis buffer waste

ISO 14001

Environmental management system certified production

Container Recycling

Plastic container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's specialized buffer manufacturing facility employs advanced preparation technology and continuous monitoring systems to ensure consistent TBE Buffer quality and performance across all production batches.

Buffer Preparation

Precision gravimetric mixing in temperature-controlled clean rooms

Multi-point pH and conductivity verification
Quality Testing

Comprehensive electrophoretic performance validation and nuclease testing

DNA integrity verification and resolution testing
Quality Systems

ISO 9001:2015 quality management with molecular biology accreditation

Continuous improvement and method validation
Container Systems

Chemical-resistant HDPE containers with secure sealing systems

Long-term stability and contamination prevention

Market Applications & Performance Data

Comprehensive molecular biology laboratory data demonstrating TBE Buffer effectiveness across diverse electrophoresis applications with quantified performance metrics and method validations.

Molecular Biology Labs
pH Stability: 8.3 ± 0.1 maintained Resolution: 50 bp - 20 kb range Nuclease-Free: No detectable activity
Research Institutions
Publication Support: 1500+ electrophoresis citations Reproducibility: >99% consistent separation Innovation: Custom buffer formulations
Diagnostic Labs
Reliability: 99.9% batch consistency Regulatory: 100% validation support Efficiency: 20% faster separations

DRAVYOM Competitive Advantages

Superior Buffer Quality

Consistently exceeds molecular biology specifications with exceptional pH stability and nuclease-free performance

Reliable Supply

Guaranteed availability with strategic inventory management and specialized buffer production

Electrophoresis Expertise

Dedicated molecular biology team provides separation optimization and troubleshooting support

Quality Assurance

Traceable certificates with comprehensive buffer characterization and performance data

Global Standards

International compliance with molecular biology buffer specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with molecular biology laboratories and custom buffer development