PCR Master Mix Components - High Performance Molecular Biology Reagents
PCR Grade Premium
Analytical Reagent

PCR Master Mix Components

Premium PCR master mix components engineered for demanding molecular biology applications including PCR amplification, qPCR, genotyping, and cloning. Our high-performance reagents deliver exceptional specificity, consistent performance, and reliable results for critical molecular processes requiring superior amplification efficiency with stringent quality control standards.

  • High-Performance PCR Grade
  • Superior Amplification Efficiency
  • Complete Component Set
  • Optimized Buffer System
  • qPCR Compatible
  • High Fidelity Performance
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Technical Specifications

Product Type: PCR Master Mix Components
Grade: PCR/qPCR Grade
DNA Polymerase: High Fidelity Taq
Buffer System: Optimized 10X PCR Buffer
MgCl₂ Concentration: 25 mM
dNTP Mix: 10 mM each (dATP, dCTP, dGTP, dTTP)
Amplification Range: Up to 10 kb
Error Rate: 2.8 × 10⁻⁶ errors/bp/cycle
3' to 5' Exonuclease: Yes (proofreading activity)
Hotstart Capability: Available
Storage Temperature: -20°C
Shelf Life: 24 months
Kit Size: 100, 500, 1000 reactions
Quality Control: Functional assay tested

Applications

PCR Amplification
qPCR Analysis
Real-Time PCR
Genotyping
Cloning Applications
Mutation Detection
Gene Expression Analysis
Research Applications
Diagnostic PCR
High-Throughput Screening
SNP Analysis
Clinical Diagnostics

Industry-Specific Grades

DRAVYOM offers specialized PCR master mix component grades tailored for specific molecular biology requirements, ensuring optimal performance and regulatory compliance across diverse research and diagnostic applications.

Research Grade
Purity: High fidelity Components: Complete set Performance: Optimized Application: Research PCR
Diagnostic Grade
Validation: IVD compatible Consistency: Batch tested Traceability: Complete Application: Clinical diagnostics
HotStart Grade
Activation: Heat-activated Specificity: Enhanced Background: Reduced Application: Sensitive PCR
High Fidelity Grade
Error Rate: Ultra-low Proofreading: 3' to 5' exonuclease Accuracy: Superior Application: Cloning, sequencing

Quality Standards

DRAVYOM's PCR Master Mix Components are manufactured under stringent quality control protocols, meeting international molecular biology standards including ISO 13485 and GMP guidelines. Our molecular biology-grade production ensures consistent performance and regulatory compliance.

ISO 13485 Certified Manufacturing
PCR Grade Specifications
High Fidelity Performance
Functional Assay Testing
Superior Amplification
Batch-to-Batch Consistency
Certificate of Analysis
Cold Chain Storage

Advanced Chemical Properties & Performance

AR Grade PCR Master Mix Components exhibit exceptional molecular biology properties essential for polymerase chain reaction applications. The ultra-pure composition and optimized formulation ensure reliable performance in demanding PCR procedures and amplification protocols.

PCR Properties
Taq Polymerase: 5 U/μL thermostable
dNTP Mix: 10 mM each (dATP, dCTP, dGTP, dTTP)
MgCl₂ Concentration: 25 mM
Buffer pH: 8.3 ± 0.1 at 25°C
Thermal Properties
Activity Temperature: 72°C optimal
Half-life (95°C): 40 minutes
Storage Temperature: -20°C
Freeze-Thaw Cycles: ≥50 cycles stable
Performance Metrics
Amplification Efficiency: 90-110%
Sensitivity: 1-10 copies detection
Specificity: High fidelity amplification
Product Length: Up to 5 kb
Purity Specifications
DNase Activity: Not detected
RNase Activity: Not detected
Endotoxin Level: ≤0.1 EU/μL
Contaminating DNA: Not detected
Stability Properties
Shelf Life (-20°C): 2 years
Working Solution (4°C): 1 week stable
Room Temperature: 24 hours max
Transport Stability: Dry ice required

Performance Characteristics

Detailed performance metrics demonstrate AR Grade PCR Master Mix Components superiority in molecular biology applications with exceptional amplification efficiency, high sensitivity, and reproducibility across diverse PCR procedures and amplification protocols.

Amplification

Efficiency: 90-110% optimal

Superior PCR performance
Sensitivity

Detection: 1-10 copies

Ultra-high sensitivity
Purity

Contaminants: Not detected

Molecular biology grade
Thermal Stability

Half-life: 40 min at 95°C

Excellent heat resistance
Reproducibility

CV: ≤5% between runs

Outstanding consistency
Shelf Stability

2 years at -20°C

Extended molecular biology integrity

Safety Information

Molecular biology reagents with minimal safety concerns. Handle with standard laboratory precautions including gloves and safety goggles. Avoid contamination and maintain sterile technique. Follow biosafety guidelines for molecular biology work.

Low Hazard
Molecular Biology
Sterile Handling

Storage & Handling

Store at -20°C in original containers. Avoid repeated freeze-thaw cycles. Thaw on ice and mix gently before use. Maintain sterile technique and use sterile tips and tubes for handling.

-20°C freezer storage
Sterile technique required
Original sterile containers
Contamination prevention

Chemical Mechanisms & Reaction Pathways

PCR Master Mix components exhibit optimized enzymatic activity and buffer stability through carefully balanced molecular mechanisms, enabling reliable DNA amplification and precise analytical applications.

DNA Polymerase Activity

5' to 3' polymerase activity with 3' to 5' exonuclease proofreading

High-fidelity DNA synthesis
Thermal Stability

Thermostable enzyme activity at 95°C denaturation cycles

Reliable PCR performance
Buffer Optimization

pH buffering and ionic strength control for optimal enzyme activity

Consistent amplification conditions
dNTP Incorporation

Balanced nucleotide availability for efficient DNA synthesis

Uniform amplification kinetics

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global molecular biology laboratory access with complete documentation packages supporting international standards and molecular biology method validations.

Molecular Biology Grade

Certified for molecular biology applications with DNase/RNase-free guarantee

ISO 13485 Standard

Medical device quality management for diagnostic applications

Research Use Only

Validated for research applications with comprehensive performance data

CE Marking Available

European conformity marking for diagnostic applications

FDA Guidelines

Manufactured following FDA guidelines for molecular diagnostics

SDS Documentation

Multi-language Safety Data Sheets with molecular biology handling protocols

Technical Support & Value-Added Services

DRAVYOM's molecular biology team provides comprehensive PCR optimization support, troubleshooting assistance, and analytical services to optimize AR Grade PCR Master Mix performance in your specific applications.

PCR Optimization
  • Thermal cycling protocol development
  • Primer design and optimization
  • Template concentration guidance
  • Multiplex PCR development
Quality Services
  • Certificate of Analysis verification
  • Custom activity testing
  • Contamination testing
  • Stability validation
Technical Support
  • PCR troubleshooting consultation
  • Method transfer support
  • Protocol development assistance
  • Laboratory setup guidance
Custom Services
  • Custom master mix formulations
  • Bulk supply arrangements
  • Private labeling options
  • Application-specific development

Environmental Impact & Sustainability

Our AR Grade PCR Master Mix production emphasizes environmental responsibility through sustainable biotechnology practices, waste reduction, and comprehensive environmental impact management for molecular biology operations.

Clean Production

Biotechnology-based production with minimal chemical waste

Waste Minimization

Optimized formulations reducing packaging and waste generation

Energy Efficiency

Energy-efficient cold storage and production processes

Safe Disposal

Biodegradable components with safe disposal protocols

Green Certification

Environmentally conscious production with sustainability metrics

Cold Chain Management

Optimized cold storage reducing energy consumption

Manufacturing Excellence & Quality Control

DRAVYOM's specialized molecular biology manufacturing facility employs advanced biotechnology with continuous monitoring systems to ensure consistent AR Grade PCR Master Mix quality and performance.

Production Process

Sterile biotechnology production with automated formulation systems

Precise component ratios and activity control
Quality Testing

25-point testing protocol including enzyme activity and contamination

Real-time PCR validation and sterility testing
Quality Systems

ISO 13485 quality management with molecular biology laboratory accreditation

Continuous improvement and method validation
Cold Chain Control

Temperature-controlled packaging with freeze-thaw stability testing

Maintained enzyme activity and performance

Market Applications & Performance Data

Comprehensive molecular biology laboratory data demonstrating AR Grade PCR Master Mix effectiveness across diverse applications with quantified performance metrics and method validations.

PCR Applications
Amplification Efficiency: >95% per cycle Sensitivity: 1-10 copies template DNA Specificity: >99.9% target specificity
Research Institutions
Publication Support: 1200+ cited protocols Reproducibility: >99.8% inter-lab consistency Innovation: Advanced PCR applications
Diagnostic Labs
Reliability: 99.9% batch consistency Validation: 100% method validation success Efficiency: 50% faster setup time

DRAVYOM Competitive Advantages

Superior PCR Performance

Consistently exceeds specifications with exceptional amplification efficiency and specificity

Cold Chain Excellence

Guaranteed cold storage with temperature monitoring and stability validation

Molecular Biology Expertise

Dedicated PCR team provides method development and optimization support

Quality Assurance

Comprehensive certificates with PCR performance data and method validation support

Global Standards

International compliance with molecular biology standards for worldwide acceptance

Custom Solutions

Collaborative development of custom PCR formulations and application-specific solutions