Ar Methylene Blue
Analytical Vital Stain
Analytical Reagent

Methylene Blue

Analytical Reagent grade Methylene Blue manufactured for staining, microscopy, and analytical chemistry. Our AR grade vital stain provides reliable staining properties for biological specimens, cell viability studies, and histological examinations in research and clinical applications.

  • Analytical Grade Purity
  • Vital Staining
  • Cell Viability Studies
  • Microscopy Applications
  • Histological Staining
  • Research Laboratory Use
Request Quote Technical Support

Technical Specifications

Chemical Formula: C₁₆H₁₈ClN₃S
CAS Number: 61-73-4
EINECS Number: 200-515-2
Assay (Dye Content): ≥82.0% (AR Grade)
Physical State: Dark green crystalline powder
Bulk Density: 0.50-0.70 g/cm³
Molecular Weight: 319.85 g/mol
Melting Point: 100-110°C (with decomposition)
λ max (Absorption): 664 nm (in water)
Solubility (water, 20°C): 43.6 g/L
Water Content: ≤ 12.0%
Residue on Ignition: ≤ 0.2%
Zinc Content: ≤ 50 ppm
Heavy Metals (as Pb): ≤ 0.002%
Methylene Violet: ≤ 4.0%
pH (1% solution): 3.0-4.5
Light Stability: Good (store in dark)
Packaging Options: 25g, 100g, 250g amber glass bottles

Applications

Vital Staining
Nucleic Acid Staining
Cell Viability Testing
Bacterial Identification
Redox Indicator
Pharmaceutical Testing
Environmental Analysis
Educational Laboratories
Histopathology
Quality Control
Methemoglobin Treatment
Reference Standard

Industry-Specific Grades

DRAVYOM offers specialized methylene blue grades tailored for specific analytical requirements, ensuring optimal performance and regulatory compliance across diverse laboratory applications.

AR Grade (Analytical Reagent)
Dye Content: ≥82.0% Residue: ≤ 0.2% Heavy Metals: ≤ 0.002% Application: General analytical procedures
Biological Stain
Dye Content: ≥85.0% CI Number: 52015 Methylene Violet: ≤ 3.0% Application: Microscopy and staining
USP Grade
Dye Content: ≥84.0% USP Monograph: Compliant Pharmaceutical Grade: Yes Application: Medical/pharmaceutical use
Extra Pure Grade
Dye Content: ≥90.0% Low Metal Content: Yes High Purity: Certified Application: Research applications

Quality Standards

DRAVYOM's AR Grade Methylene Blue is manufactured under stringent quality control protocols, meeting international analytical standards including ACS, ISO, and pharmacopoeia specifications. Our analytical-grade production ensures consistent staining performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
USP Grade Specifications
≥82.0% Dye Content
Advanced Analytical Testing
Superior Staining Properties
Batch-to-Batch Consistency
Traceable Certificate of Analysis
Light-Protected Storage

Advanced Chemical Properties & Performance

AR Grade Methylene Blue exhibits exceptional dye properties essential for biological staining applications. Its high purity thiazine structure and precise chromophore composition ensure reliable performance in vital staining and redox indicator procedures.

Staining Properties
Chemical Class: Thiazine cationic dye
Absorption Maximum: 664 nm (aqueous solution)
Molar Absorptivity: 81,000 L·mol⁻¹·cm⁻¹
Staining Affinity: Nucleic acids, bacteria
Redox Properties
Redox Potential: +0.532 V (vs SHE)
Reduced Form: Colorless leuco base
Reversibility: Complete oxidation-reduction
pH Sensitivity: Stable pH 4-10 range
Analytical Performance
Detection Limit: 0.1 μg/mL spectrophotometric
Linearity Range: 0.1-20 mg/L (Beer's law)
Staining Time: 30 seconds to 5 minutes
Contrast Quality: High nuclear-cytoplasm distinction

Performance Characteristics

Detailed performance metrics demonstrate Methylene Blue superiority in biological applications with exceptional staining intensity, redox indicator reliability, and reproducibility across diverse microscopy and analytical procedures.

Staining Intensity

Absorption: 81,000 L·mol⁻¹·cm⁻¹ coefficient

Exceptional color intensity
Microscopy Quality

Resolution: Subcellular detail visible

Superior imaging contrast
Redox Reliability

Potential: +0.532 V stable reference

Consistent electrochemical response
Rapid Response

Time: 30 seconds staining capability

Fast laboratory workflow

Safety Information

Low toxicity dye but may cause eye and skin irritation. Avoid ingestion and inhalation of powder. Use appropriate protective equipment including gloves, safety goggles, and lab coats. Ensure adequate ventilation and follow standard laboratory safety protocols.

Eye Irritant
Skin Contact
Avoid Inhalation

Storage & Handling

Store in original containers in a cool, dry, dark place away from strong oxidizing agents and light sources. Keep containers tightly closed to prevent moisture absorption and contamination. Use appropriate measuring equipment to prevent cross-contamination.

Cool storage (15-25°C)
Protect from light exposure
Moisture-proof containers
Away from oxidizing agents

Chemical Mechanisms & Reaction Pathways

Methylene blue functions through redox mechanisms and cellular staining properties, enabling vital staining, oxidation-reduction reactions, and antimicrobial activity with predictable chemical pathways.

Nucleic Acid Binding

Intercalation between DNA base pairs producing deep blue nuclear staining

Chromatin visualization and cell identification
Redox Activity

Reversible oxidation-reduction between methylene blue and leuco form

Electron transport chain studies
Antimicrobial Action

Photodynamic antimicrobial activity through singlet oxygen generation

Light-activated disinfection
Vital Staining

Live cell staining without immediate cytotoxic effects

Cell viability assessment

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global laboratory access with complete documentation packages supporting international analytical standards and clinical method validations.

USP Standards

United States Pharmacopeia methylene blue specifications

Clinical Standards

Clinical laboratory diagnostic staining compliance

ACS Grade Available

American Chemical Society analytical reagent standards

REACH Registration

European Union chemical regulation compliance

FDA Method Support

Validated for FDA pharmaceutical testing methods

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's analytical chemistry team provides comprehensive staining method support, troubleshooting assistance, and analytical services to optimize methylene blue performance in your specific applications.

Staining Method Development
  • Staining protocol optimization
  • Concentration adjustment guidance
  • pH and timing optimization
  • Quality control procedures
Analytical Services
  • Dye purity verification
  • Staining quality assessment
  • Stability testing protocols
  • Method validation support
Technical Support
  • Staining troubleshooting consultation
  • Safe handling and preparation training
  • Laboratory protocol development
  • Quality assurance guidance
Supply Solutions
  • Consistent dye quality batching
  • Emergency supply arrangements
  • Custom concentration solutions
  • Global distribution network

Environmental Impact & Sustainability

Our methylene blue production emphasizes environmental responsibility through sustainable manufacturing practices, waste reduction systems, and comprehensive environmental impact management for laboratory operations.

Dye Recovery

Advanced dye recovery and purification systems

Waste Minimization

Optimized synthesis reducing waste generation

Clean Production

Energy-efficient manufacturing with emission controls

Safe Disposal

Comprehensive neutralization and disposal guidance

ISO 14001

Environmental management system certified production

Container Recycling

Glass container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art dye manufacturing facility employs advanced synthesis technology with continuous monitoring systems to ensure consistent methylene blue quality and performance across all production batches.

Synthesis Process

Controlled oxidation synthesis with precise purification protocols

Consistent dye purity and staining performance
Quality Testing

Multi-point validation including purity, staining, and spectral analysis

UV-Vis spectrophotometry and microscopic verification
Quality Systems

ISO 9001:2015 quality management with analytical laboratory compliance

Continuous improvement and method validation
Packaging Control

Amber glass containers with light protection and stability preservation

Chemical stability and contamination prevention

Market Applications & Performance Data

Comprehensive analytical laboratory data demonstrating methylene blue effectiveness across diverse applications with quantified performance metrics and staining method validations.

Staining Applications
Intensity: Uniform blue coloration Selectivity: Nuclear/bacterial staining Stability: 18-month shelf life
Research Institutions
Publication Support: 500+ cited protocols Reproducibility: >99% consistent results Innovation: Advanced staining techniques
Clinical Laboratories
Reliability: 99.8% batch consistency Efficiency: Rapid staining protocols Safety: Low toxicity formulation

DRAVYOM Competitive Advantages

Superior Dye Quality

Consistently high-purity methylene blue with optimal staining intensity and color stability

Reliable Supply

Guaranteed availability with strategic inventory management and consistent production scheduling

Analytical Expertise

Dedicated analytical chemistry team provides staining method development and technical support

Quality Assurance

Traceable certificates with comprehensive staining validation and analytical method support

Global Standards

International compliance with USP, clinical, and analytical specifications for worldwide acceptance

Partnership Approach

Collaborative relationships with research institutions and custom staining solution development services