Ar Grams Stain Solutions
Analytical Microbiology
Analytical Reagent

Gram's Stain Solutions (Crystal Violet, Iodine, Safranin)

Analytical Reagent grade Gram's Stain Solutions manufactured for bacterial identification, microbiology, and differential staining. Our AR grade complete staining kit provides reliable bacterial differentiation properties for gram-positive and gram-negative identification in clinical diagnostics and research applications.

  • Analytical Grade Purity
  • Complete Staining Kit
  • Bacterial Identification
  • Differential Staining
  • Clinical Microbiology
  • Research Applications
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Technical Specifications

Stain Kit Components: 4-solution complete set
Primary Stain: Crystal Violet (0.5% w/v)
Mordant: Gram's Iodine solution
Decolorizer: Ethyl alcohol (95%)
Counter Stain: Safranin (0.25% w/v)
Crystal Violet CAS: 548-62-9
Safranin CAS: 477-73-6
Staining Time: 4-6 minutes total
Storage Temperature: 15-30°C
Shelf Life: 24 months from manufacture
Gram-Positive Result: Blue to purple
Gram-Negative Result: Pink to red
Sensitivity: 10³ CFU/ml minimum
Packaging: 4 × 100ml bottle set

Applications

Bacterial Classification
Clinical Microbiology
Infection Diagnosis
Antibiotic Selection
Rapid Identification
Medical Diagnostics
Educational Microbiology
Quality Control
Research Applications
Analytical Testing
Cell Wall Analysis
Environmental Microbiology

Industry-Specific Grades

DRAVYOM offers specialized Gram's stain solution grades tailored for specific diagnostic and research requirements, ensuring optimal performance and regulatory compliance across diverse laboratory applications.

AR Grade (Analytical Reagent)
Crystal Violet: 0.5% w/v Safranin: 0.25% w/v Clarity: Excellent Application: General bacterial staining
Clinical Grade
USP/EP Compliant Performance: QC validated Differentiation: Sharp Application: Clinical diagnostics
Research Grade
High Purity Components Batch Consistency: Guaranteed Color Contrast: Superior Application: Advanced research
Rapid Stain Grade
Staining Time: 2-3 minutes High Throughput: Suitable Emergency Use: Validated Application: Rapid diagnostics

Quality Standards

DRAVYOM's AR Grade Gram's Stain Solutions are manufactured under stringent quality control protocols, meeting international diagnostic standards including USP, EP, and clinical laboratory specifications. Our analytical-grade production ensures consistent performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Clinical Laboratory Standards
Precise Dye Concentrations
Differentiation Validation Testing
Sharp Gram Differentiation
Batch-to-Batch Consistency
Traceable Certificate of Analysis
Temperature-Controlled Logistics

Advanced Chemical Properties & Performance

AR Grade Gram's Stain Solutions exhibit exceptional differential staining properties essential for bacterial classification and identification. The ultra-pure primary stain, mordant, decolorizer, and counterstain composition ensures reliable performance in microbiological diagnosis requiring precise gram-positive and gram-negative bacterial differentiation.

Staining Components
Primary Stain: Crystal violet solution
Mordant: Iodine solution
Decolorizer: Ethanol or acetone-ethanol
Counterstain: Safranin solution
Physical Properties
Crystal Violet: Deep purple solution
Iodine Solution: Brown solution
Decolorizer: Clear, volatile solution
Safranin: Red solution
Staining Performance
Gram-Positive: Retains purple color
Gram-Negative: Pink/red counterstain
Staining Time: 4-6 minutes total
Differentiation: Clear morphological distinction
Quality Specifications
Crystal Violet Purity: ≥90% active dye
Iodine Content: 1% w/v
Ethanol Purity: ≥95%
Safranin Purity: ≥85% active dye
Stability Properties
Shelf Life: 2 years (unopened)
Working Life: 6 months after opening
Light Sensitivity: Store in dark
Evaporation Rate: Decolorizer moderately volatile

Performance Characteristics

Detailed performance metrics demonstrate AR Grade Gram's Stain Solutions superiority in bacterial classification with exceptional differential staining, reliable morphological identification, and reproducible results across diverse clinical microbiology applications.

Classification Accuracy

Differentiation: >98% gram classification accuracy

Superior bacterial identification
Color Contrast

Visual distinction: Clear purple vs pink

Excellent morphological contrast
Staining Speed

Procedure time: 4-6 minutes total

Rapid diagnostic results
Decolorization Control

Differential action: Precise gram distinction

Controlled selective removal
Batch Consistency

Variation: ±2% between batches

Outstanding lot-to-lot reproducibility
Resolution Quality

Morphology: Clear cellular detail

High-resolution bacterial imaging

Safety Information

Contains ethanol and iodine - flammable and potentially irritating components. May cause eye and skin irritation. Use appropriate protective equipment including safety goggles and gloves. Ensure adequate ventilation and keep away from ignition sources. Handle iodine solution with care to avoid staining.

Flammable
Irritant
Eye Protection Required

Storage & Handling

Store all solutions in original containers in a cool, dry, dark place away from heat sources. Keep decolorizer tightly sealed to prevent evaporation. Store iodine solution in amber bottles to prevent degradation. Use appropriate ventilation when handling volatile components.

Cool storage (15-25°C)
Protect from light
Amber bottles for iodine
Away from ignition sources

Chemical Mechanisms & Reaction Pathways

Gram's stain solutions function through differential cell wall binding mechanisms, enabling bacterial classification through peptidoglycan interaction pathways and selective dye retention for microbiological identification.

Primary Stain Binding

Crystal violet penetrates both Gram-positive and Gram-negative cell walls

Initial purple coloration of all bacterial cells
Iodine Complexation

Iodine forms complexes with crystal violet, fixing the dye in cell walls

Enhanced dye retention through complex formation
Decolorization Selectivity

Alcohol removes dye from thin peptidoglycan layers but not thick ones

Critical step for Gram-positive/negative differentiation
Counterstain Mechanism

Safranin provides contrasting color to decolorized cells

Pink counterstain for Gram-negative bacteria

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global clinical microbiology laboratory access with complete documentation packages supporting international bacterial identification standards and diagnostic validations.

CLSI Standards

Clinical and Laboratory Standards Institute microbiology protocols

ASM Guidelines

American Society for Microbiology staining procedures

FDA Compliance

Food and Drug Administration diagnostic staining requirements

REACH Registration

European Union chemical regulation compliance

WHO Standards

World Health Organization microbiology guidelines support

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's microbiology team provides comprehensive Gram staining support, bacterial identification optimization, and diagnostic method development services for clinical and research microbiology applications.

Staining Protocols
  • Gram staining procedure optimization
  • Timing and temperature control
  • Decolorization step enhancement
  • Quality control standardization
Diagnostic Services
  • Stain kit performance verification
  • Bacterial identification validation
  • Microscopy technique support
  • Morphological assessment training
Technical Support
  • Clinical microbiology consultation
  • Troubleshooting guidance
  • Staff training programs
  • Quality assurance protocols
Supply Solutions
  • Complete staining kit assemblies
  • Custom reagent concentrations
  • Stability-tested formulations
  • Global microbiology supply network

Environmental Impact & Sustainability

Our Gram's stain production emphasizes environmental responsibility through sustainable dye chemistry practices, minimal waste generation, and comprehensive environmental impact management for diagnostic applications.

Clean Production

Environmentally conscious dye preparation and solution formulation

Solvent Recovery

Alcohol recovery programs and organic solvent recycling systems

Efficient Manufacturing

Optimized staining solution preparation with minimal environmental impact

Safe Disposal

Comprehensive stain waste disposal and neutralization guidance

ISO 14001

Environmental management system certified production

Container Recycling

Glass container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's specialized diagnostic staining facility employs advanced multi-component formulation technology with continuous monitoring systems to ensure consistent Gram's stain quality and diagnostic performance across all production batches.

Multi-Component Formulation

Precise preparation of crystal violet, iodine, alcohol, and safranin solutions

Accurate concentration control and component compatibility
Quality Testing

19-point testing including dye content, pH, and staining performance

Bacterial testing and microscopic verification
Quality Systems

ISO 9001:2015 quality management with microbiological validation

Continuous improvement and batch consistency monitoring
Packaging Control

Individual component bottles with proper labeling and closure systems

Cross-contamination prevention and stability preservation

Market Applications & Performance Data

Comprehensive clinical microbiology data demonstrating Gram's stain effectiveness across diverse bacterial identification applications with quantified performance metrics and diagnostic validations.

Clinical Applications
Identification Accuracy: >99% correct Gram classification Staining Consistency: Uniform color differentiation Stability: 24+ months shelf life per component
Research Institutions
Publications: 500+ cited microbiology procedures Reproducibility: >99.5% inter-lab agreement Innovation: Advanced bacterial identification protocols
Clinical Labs
Reliability: 99.9% batch consistency Audit Success: 100% regulatory compliance Efficiency: 25% faster bacterial identification

DRAVYOM Competitive Advantages

Superior Staining Performance

Consistently clear Gram-positive/negative differentiation with exceptional bacterial morphology visualization for accurate identification

Reliable Supply

Guaranteed availability with strategic inventory management and complete staining kit production scheduling

Microbiology Expertise

Dedicated bacterial identification team provides staining method development and diagnostic troubleshooting support

Quality Assurance

Traceable certificates with comprehensive staining performance data and microbiological validation support

Global Standards

International compliance with CLSI, ASM, and WHO specifications for worldwide clinical acceptance

Partnership Approach

Collaborative relationships with clinical microbiology laboratories and custom bacterial identification method development services