DAPI Stain - A.R. Grade DNA Fluorescent Dye
A.R. Grade Premium
Analytical Reagent

A.R. Grade DAPI Stain

Premium analytical grade DAPI stain engineered for demanding molecular biology applications including DNA visualization, nuclei staining, and fluorescence microscopy. Our fluorescent dye delivers exceptional purity, consistent performance, and reliable results for critical analytical processes requiring superior staining quality with stringent quality control standards.

  • High Purity A.R. Grade Quality
  • Superior DNA Binding Specificity
  • Bright Blue Fluorescence
  • Consistent Batch-to-Batch Quality
  • Fluorescence Microscopy Compatible
  • Cell Biology Applications
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Technical Specifications

Chemical Name: 4',6-Diamidino-2-phenylindole
Chemical Formula: C₁₆H₁₅N₅
CAS Number: 28718-90-3
Molecular Weight: 277.33 g/mol
Purity (HPLC): ≥98.0%
Physical State: Yellow crystalline powder
Excitation Maximum: 358 nm
Emission Maximum: 461 nm (blue)
Working Concentration: 0.1-1.0 μg/mL
Solubility: Water, methanol, DMSO
Storage Temperature: -20°C (protect from light)
Stability: 2 years at -20°C
Fluorescence Quantum Yield: 0.43 (bound to DNA)
Packaging Options: 1mg, 5mg, 10mg vials

Applications

DNA Staining
Fluorescence Microscopy
Cell Nucleus Visualization
Flow Cytometry
Chromosome Analysis
Live Cell Imaging
Apoptosis Detection
Research Applications
Immunofluorescence
Cell Biology Research
Confocal Microscopy
Medical Diagnostics

Industry-Specific Grades

DRAVYOM offers specialized DAPI stain grades tailored for specific analytical and research requirements, ensuring optimal performance and regulatory compliance across diverse laboratory applications.

Research Grade
Purity: ≥98.0% Fluorescence: High quantum yield Background: Low Application: General microscopy
Certified Grade
Purity: ≥99.0% Certificate: CoA provided Traceability: Batch records Application: Validated methods
Ultra-Pure Grade
Purity: ≥99.5% Impurities: Minimized Fluorescence: Maximum intensity Application: Critical imaging
GMP Grade
Purity: ≥99.0% Manufacturing: GMP facility Documentation: Complete Application: Clinical research

Quality Standards

DRAVYOM's DAPI Stain is manufactured under stringent quality control protocols, meeting international analytical standards including research-grade specifications and GMP requirements. Our fluorescent dye production ensures consistent performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
Research Grade Specifications
≥98.0% HPLC Purity
Advanced Fluorescence Testing
Superior Imaging Quality
Batch-to-Batch Consistency
Certificate of Analysis
Cold Storage Logistics

Advanced Chemical Properties & Performance

AR Grade DAPI Stain exhibits exceptional fluorescent properties essential for analytical applications. Its ultra-pure composition and precise spectral characteristics ensure reliable performance in demanding microscopy procedures and analytical determinations.

Fluorescent Properties
Excitation Maximum: 358 nm
Emission Maximum: 461 nm
Quantum Yield: 0.27 (bound to DNA)
Binding Specificity: AT-rich DNA regions
Physical Properties
Appearance: Yellow crystalline powder
Molecular Weight: 350.25 g/mol
Solubility: 14 mg/mL in water
Melting Point: >300°C (decomposes)
Analytical Performance
Staining Efficiency: 100% nuclei visualization
Signal-to-Noise: 50:1 ratio
Photobleaching: Minimal under standard conditions
Background Signal: ≤2% of specific binding
Purity Specifications
Chemical Purity: ≥98% by HPLC
Fluorescent Impurities: ≤0.5%
Water Content: ≤5%
Heavy Metals: ≤10 ppm
Stability Properties
Shelf Life: 2 years (unopened)
Light Stability: Protect from light
Solution Stability: 6 months at 4°C
Freeze-Thaw: Stable for 5 cycles

Performance Characteristics

Detailed performance metrics demonstrate AR Grade DAPI Stain superiority in analytical applications with exceptional fluorescent intensity, high specificity, and reproducibility across diverse microscopy procedures and testing protocols.

Fluorescent Performance

Intensity: 50-fold increase upon DNA binding

Superior fluorescent enhancement
Staining Precision

Consistency: ±5% fluorescence variation

Exceptional staining reliability
Binding Specificity

Selectivity: >95% nuclear localization

High DNA specificity
Temperature Range

Working range: 4-37°C optimal

Physiological temperature stability
Batch Reproducibility

Variation: ±3% between batches

Outstanding lot-to-lot consistency
Shelf Stability

2 years under proper storage conditions

Extended analytical grade integrity

Safety Information

Potentially mutagenic compound. Handle with appropriate protective equipment including gloves, safety goggles, and use in well-ventilated areas. Avoid skin contact and inhalation. Use proper laboratory safety procedures and dispose according to hazardous waste protocols.

Potential Mutagen
Eye Protection Required
Glove Protection Required

Storage & Handling

Store in original containers at -20°C in a dry, dark environment protected from light. Keep containers tightly closed and use appropriate cold storage equipment. Maintain temperature monitoring and protect from freeze-thaw cycles.

Frozen storage (-20°C)
Dark, dry environment
Light-proof containers
Avoid freeze-thaw cycles

Chemical Mechanisms & Reaction Pathways

DAPI exhibits controlled fluorescent binding mechanisms through DNA intercalation, enabling precise analytical applications for nuclear staining and fluorescence microscopy procedures with quantifiable fluorescent responses.

DNA Intercalation

Selective binding to AT-rich DNA regions with enhanced fluorescence

Specific nuclear localization and staining
Fluorescence Enhancement

100-fold fluorescence increase upon DNA binding (358/461 nm)

High-contrast nuclear visualization
Membrane Permeability

Cell membrane penetration enabling live and fixed cell staining

Versatile staining methodology
Photostability

Resistance to photobleaching for extended imaging sessions

Reliable long-term fluorescence maintenance

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global research laboratory access with complete documentation packages supporting international standards and fluorescence microscopy method validations.

Research Grade Standards

High-purity fluorescent dye specifications for research applications

ISO 6353 Standard

International standard for reagent specifications and testing

Laboratory Standards

Analytical grade specifications for laboratory research

REACH Registration

European Union chemical regulation compliance

Research Method Support

Validated for microscopy and cell biology methods

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's fluorescence microscopy team provides comprehensive DAPI staining support, troubleshooting assistance, and analytical services to optimize AR Grade DAPI performance in your specific research applications.

Staining Method Development
  • DAPI staining optimization protocols
  • Concentration and incubation guidance
  • Fixation and permeabilization optimization
  • Microscopy imaging parameter setup
Analytical Services
  • Certificate of Analysis verification
  • Custom purity analysis
  • Fluorescence efficiency testing
  • Stability validation
Technical Support
  • Fluorescence troubleshooting consultation
  • Safe handling and storage training
  • Method transfer support
  • Laboratory safety protocols
Supply Solutions
  • Consistent high-purity fluorescent batching
  • Cold chain distribution arrangements
  • Custom packaging solutions
  • Global frozen storage network

Environmental Impact & Sustainability

Our AR Grade DAPI production emphasizes environmental responsibility through sustainable manufacturing practices, waste minimization, and comprehensive environmental impact management for research laboratory operations.

Clean Production

Controlled synthesis environment with minimal organic waste generation

Solvent Recovery

Efficient solvent recycling and purification programs

Sustainable Process

Energy-efficient synthesis with emission controls

Safe Disposal

Comprehensive fluorescent compound treatment and disposal guidance

ISO 14001

Environmental management system certified production

Cold Storage Efficiency

Energy-efficient frozen storage management systems

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art fluorescent compound manufacturing facility employs advanced synthesis technology with continuous monitoring systems to ensure consistent AR Grade DAPI quality and performance across all production batches.

Production Process

Multi-step organic synthesis with ultra-pure starting materials

Precise molecular structure control and purification
Quality Testing

12-point fluorescence testing protocol including purity, spectral properties

HPLC verification and fluorescence spectroscopy
Quality Systems

ISO 9001:2015 quality management with research laboratory accreditation

Continuous improvement and fluorescence method validation
Packaging Control

Light-proof containers with nitrogen atmosphere and frozen storage compatibility

Fluorescence stability and contamination prevention

Market Applications & Performance Data

Comprehensive research laboratory data demonstrating AR Grade DAPI effectiveness across diverse applications with quantified performance metrics and fluorescence microscopy validations.

Fluorescence Applications
Precision: 100-fold fluorescence enhancement Selectivity: AT-rich DNA specificity Stability: 12-month frozen storage maintenance
Research Institutions
Publication Support: 1000+ cited methods Reproducibility: >99.5% inter-lab agreement Innovation: Advanced fluorescence protocols
Core Facilities
Reliability: 99.8% batch consistency Performance: Consistent imaging results Efficiency: 40% faster staining procedures

DRAVYOM Competitive Advantages

Superior Fluorescence Quality

Consistently exceeds specifications with exceptional brightness and photostability for research applications

Cold Chain Reliability

Guaranteed frozen storage with strategic inventory management and temperature-controlled distribution

Fluorescence Expertise

Dedicated fluorescence chemistry team provides staining method development and troubleshooting support

Quality Assurance

Traceable certificates with comprehensive fluorescence data and method validation support

Research Standards

High-purity specifications meeting international research laboratory requirements worldwide

Partnership Approach

Collaborative relationships with research laboratories and custom fluorescent compound development services