Ar Bile Salt Broth
Analytical Selective
Analytical Reagent

Culture Media: Bile Salt Broth

Analytical Reagent grade Bile Salt Broth manufactured for gram-negative bacterial enrichment, enterobacteria culture, and clinical microbiology. Our AR grade selective medium provides reliable enrichment properties for pathogenic bacteria detection, clinical diagnostics, and research applications.

  • Analytical Grade Purity
  • Selective Enrichment
  • Gram-Negative Culture
  • Enterobacteria Isolation
  • Clinical Microbiology
  • Research Laboratory Use
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Technical Specifications

Medium Type: Selective differential broth
Primary Components: Peptone, bile salts, glucose
Bile Salt Concentration: 0.5% w/v
pH (25°C): 7.2 ± 0.2
Physical State: Dehydrated powder
Color: Light yellow to beige
Solubility: Readily soluble in distilled water
Sterilization: Autoclave at 121°C for 15 minutes
Storage Temperature: 15-30°C, dry place
Shelf Life: 36 months from manufacture
Selectivity: Gram-negative bacteria
Growth Inhibition: Gram-positive bacteria
Turbidity Standard: McFarland 0.5
Packaging: 100g, 250g, 500g bottles

Applications

Gram-Negative Isolation
Enterobacteria Enrichment
Clinical Microbiology
Medical Diagnostics
Pharmaceutical Testing
Food Safety Testing
Educational Microbiology
Quality Control
Research Applications
Water Testing
Bacterial Identification
Infection Control

Industry-Specific Grades

DRAVYOM offers specialized bile salt broth grades tailored for specific microbiological requirements, ensuring optimal performance and regulatory compliance across diverse laboratory applications.

AR Grade (Analytical Reagent)
Bile Salt: 0.5% w/v pH: 7.2±0.2 Sterility: Guaranteed Application: General microbiological procedures
Clinical Grade
USP/EP Compliant Endotoxin: <0.25 EU/g Sterility: Validated Application: Clinical diagnostics
Research Grade
High Selectivity Performance: Validated Traceability: Complete Application: Advanced research
Educational Grade
Student-Safe Formulation Easy Preparation Cost-Effective Application: Teaching laboratories

Quality Standards

DRAVYOM's AR Grade Bile Salt Broth is manufactured under stringent quality control protocols, meeting international microbiological standards including USP, EP, and clinical laboratory specifications. Our analytical-grade production ensures consistent performance and regulatory compliance.

ISO 9001:2015 Certified Manufacturing
USP/EP Grade Specifications
Precise pH Control
Microbiological Performance Testing
High Selectivity for Gram-Negatives
Batch-to-Batch Consistency
Traceable Certificate of Analysis
Temperature-Controlled Logistics

Advanced Chemical Properties & Performance

AR Grade Bile Salt Broth exhibits exceptional selective enrichment properties for coliform bacteria. Its ultra-pure composition ensures reliable performance in microbiological analysis, providing specific growth conditions for gram-negative bacteria detection.

Microbiological Properties
Selectivity: Gram-negative bacteria enrichment
Target Organisms: Coliforms, E. coli
Inhibition: Gram-positive bacteria suppression
Growth Enhancement: 2-4 fold increase in recovery
Physical Properties
Appearance: Light yellow to amber liquid
pH Range: 7.0-7.4 at 25°C
Osmolality: 280-320 mOsm/kg
Sterilization: Autoclave compatible
Performance Characteristics
Recovery Rate: ≥95% for target organisms
Incubation Time: 18-24 hours at 35°C
Detection Limit: 1-10 CFU/mL
Reproducibility: ±10% coefficient of variation
Quality Specifications
Peptone Content: Specified concentration
Bile Salts: 1.5% selective concentration
Lactose: Fermentation substrate
Sterility: Tested for contamination
Stability Properties
Shelf Life: 2 years (powder), 1 year (prepared)
Storage Temperature: 2-8°C after preparation
Light Sensitivity: Protect from direct light
pH Stability: Stable within pH 6.8-7.6

Performance Characteristics

Detailed performance metrics demonstrate AR Grade Bile Salt Broth superiority in microbiological applications with exceptional selectivity for coliforms, reliable bacterial recovery, and reproducible results across diverse water and food testing procedures.

Bacterial Recovery

Recovery rate: ≥95% for target organisms

Superior coliform enrichment
Selectivity

Gram-positive inhibition: >99%

Excellent bacterial selectivity
Growth Rate

Detection time: 18-24 hours incubation

Rapid microbiological results
Temperature Range

Optimal growth: 35±2°C

Controlled incubation conditions
Batch Consistency

Variation: ±5% between batches

Outstanding lot-to-lot reproducibility
Detection Limit

Sensitivity: 1-10 CFU/mL

High sensitivity detection

Safety Information

Microbiological medium requiring sterile handling techniques. May contain organic nutrients that can support microbial growth if contaminated. Use aseptic techniques and appropriate protective equipment including gloves, lab coats, and safety goggles. Dispose of used media according to biohazard protocols.

Biohazard Potential
Aseptic Techniques
Biohazard Disposal

Storage & Handling

Store dehydrated medium in original containers in a cool, dry place away from light. Prepared medium should be refrigerated at 2-8°C and used within recommended timeframe. Use sterile techniques during preparation and handling to prevent contamination.

Refrigerated storage (2-8°C)
Sterile handling required
Airtight containers only
Protect from light

Chemical Mechanisms & Reaction Pathways

Bile Salt Broth exhibits selective inhibition mechanisms through bile salt interactions, making it suitable for analytical applications requiring controlled bacterial differentiation and enteric pathogen isolation procedures.

Bile Salt Inhibition

Selective inhibition of gram-positive bacteria through membrane disruption

Enhanced gram-negative selectivity
Osmotic Pressure

Controlled osmotic environment favoring enteric bacteria

Selective growth conditions
pH Buffering

Peptone buffering maintaining optimal growth pH

Stable analytical conditions
Nutrient Availability

Balanced nutrient composition supporting target organism growth

Optimized media formulation
Selective Pressure

Differential growth promoting enteric pathogen enrichment

Enhanced analytical selectivity

Regulatory Compliance & Documentation

Comprehensive regulatory compliance ensures global analytical laboratory access with complete documentation packages supporting international standards and analytical method validations.

ACS Specifications

American Chemical Society analytical reagent standards compliance

ISO 6353 Standard

International standard for reagent specifications and testing

USP Grade Available

United States Pharmacopeia specifications for pharmaceutical analysis

REACH Registration

European Union chemical regulation compliance

EPA Method Support

Validated for EPA environmental analytical methods

SDS Documentation

Multi-language Safety Data Sheets (16 sections, GHS compliant)

Technical Support & Value-Added Services

DRAVYOM's analytical chemistry team provides comprehensive media method support, troubleshooting assistance, and analytical services to optimize AR Grade Bile Salt Broth performance in your specific analytical applications.

Media Method Development
  • Selective enrichment optimization protocols
  • Incubation condition development
  • pH control guidance
  • Growth enhancement techniques
Analytical Services
  • Certificate of Analysis verification
  • Custom sterility testing
  • Growth promotion validation
  • Selectivity assessment
Technical Support
  • Microbiology consultation
  • Safe handling and storage training
  • Method transfer support
  • Laboratory safety protocols
Performance Services
  • Method validation support
  • System suitability testing
  • Stability studies
  • Quality assurance consultation

Environmental Impact & Sustainability

Our AR Grade Bile Salt Broth production emphasizes environmental responsibility through sustainable manufacturing practices, controlled waste management, and comprehensive environmental impact management for analytical laboratory operations.

Media Waste Management

Proper disposal and sterilization of spent culture media

Biosafety Protocols

Comprehensive biosafety and decontamination procedures

Clean Production

Energy-efficient preparation with sterile manufacturing

Water Conservation

Optimized water usage in media preparation processes

ISO 14001

Environmental management system certified production

Container Recycling

Glass container return and recycling programs

Manufacturing Excellence & Quality Control

DRAVYOM's state-of-the-art analytical reagent manufacturing facility employs advanced media preparation technology with continuous monitoring systems to ensure consistent AR Grade Bile Salt Broth quality and performance across all production batches.

Production Process

Controlled preparation with high-purity peptone and bile salts

Precise composition control and sterile processing
Quality Testing

12-point microbiological testing protocol including sterility and growth promotion

USP sterility verification and performance testing
Quality Systems

ISO 9001:2015 quality management with microbiological laboratory accreditation

Continuous improvement and method validation
Packaging Control

Sterile packaging with controlled atmosphere protection

Sterility preservation and contamination prevention

Market Applications & Performance Data

Comprehensive analytical laboratory data demonstrating AR Grade Bile Salt Broth effectiveness across diverse applications with quantified performance metrics and analytical method validations.

Selective Enrichment
Selectivity: >90% gram-negative enrichment Growth promotion: E. coli, Salmonella, Shigella Inhibition: >95% gram-positive suppression
Growth Performance
Recovery rates: >80% for target organisms Generation time: Optimal for enteric bacteria pH stability: 7.0-7.4 maintained during growth
Sterility Assurance
Sterility: 100% sterile after autoclaving Shelf stability: 24+ months at 4°C Contamination control: <0.01% failure rate
Validation Data
Method validation: USP microbiological standards Stability: 2+ years proven shelf life Reproducibility: <0.1% RSD batch-to-batch

DRAVYOM Competitive Advantages

Ultra-Pure Media Quality

Sterile preparation, pH ±0.1, guaranteed microbiological performance

Superior Selectivity

Enhanced gram-negative enrichment with minimal false positives

Multi-Standard Compliance

ACS, USP, and ISO specifications with comprehensive documentation

Cold Chain Logistics

Consistent availability with temperature-controlled supply chain

Microbiological Expertise

Analytical chemistry support team with microbiology specialists

Environmental Responsibility

Controlled waste management with biosafety protocols